RAFTK, a novel member of the focal adhesion kinase family, is phosphorylated and associates with signaling molecules upon activation of mature T lymphocytes

Ramesh K. Ganju, William C. Hatch, Hava Avraham, Mel A. Ona, Brian Druker, Shalom Avraham, Jerome E. Groopman

Research output: Contribution to journalArticle

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Abstract

The related adhesion focal tyrosine kinase (RAFTK), a recently discovered member of the focal adhesion kinase family, has previously been reported to participate in signal transduction in neuronal cells, megakaryocytes, and B lymphocytes. We have found that RAFTK is constitutively expressed in human T cells and is rapidly phosphorylated upon the activation of the T cell receptor (TCR). This activation also results in an increase in the autophosphorylation and kinase activity of RAFTK. After its stimulation, there was an increase in the association of the src cytoplasmic tyrosine kinase Fyn and the adapter protein Grb2. This association was mediated through the SH2 domains of Fyn and Grb2. RAFTK also co-immunoprecipitates with the SH2 domain of Lck and with the cytoskeletal protein paxillin through its COOH-terminal proline-rich domain. The tyrosine phosphorylation of RAFTK after T cell receptor-mediated stimulation was reduced by the pretreatment of cells with cytochalasin D, suggesting the role of the cytoskeleton in this process. These observations indicate that RAFTK participates in T cell receptor signaling and may act to link signals from the cell surface to the cytoskeleton and thereby affect the host immune response.

Original languageEnglish (US)
Pages (from-to)1055-1063
Number of pages9
JournalJournal of Experimental Medicine
Volume185
Issue number6
DOIs
StatePublished - Mar 17 1997

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Focal Adhesion Kinase 2
Focal Adhesion Protein-Tyrosine Kinases
T-Lymphocytes
T-Cell Antigen Receptor
src Homology Domains
Cytoskeleton
Paxillin
Cytochalasin D
Cytoskeletal Proteins
Megakaryocytes
src-Family Kinases
Proline
Tyrosine
Signal Transduction
B-Lymphocytes
Phosphotransferases
Phosphorylation

ASJC Scopus subject areas

  • Immunology

Cite this

RAFTK, a novel member of the focal adhesion kinase family, is phosphorylated and associates with signaling molecules upon activation of mature T lymphocytes. / Ganju, Ramesh K.; Hatch, William C.; Avraham, Hava; Ona, Mel A.; Druker, Brian; Avraham, Shalom; Groopman, Jerome E.

In: Journal of Experimental Medicine, Vol. 185, No. 6, 17.03.1997, p. 1055-1063.

Research output: Contribution to journalArticle

Ganju, Ramesh K. ; Hatch, William C. ; Avraham, Hava ; Ona, Mel A. ; Druker, Brian ; Avraham, Shalom ; Groopman, Jerome E. / RAFTK, a novel member of the focal adhesion kinase family, is phosphorylated and associates with signaling molecules upon activation of mature T lymphocytes. In: Journal of Experimental Medicine. 1997 ; Vol. 185, No. 6. pp. 1055-1063.
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