Radioimmunoassay and metabolism of the catechol estrogen 2-hydroxyestradiol

Shinzo Kono, George R. Merriam, David D. Brandon, Donald (Lynn) Loriaux, Mortimer B. Lipsett

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Plasma levels of 2-hydroxyestradiol (2-OHE2)were measured using a new RIA procedure. Values were below the detection limit of the assay (<10 pg/ml), except in the third trimester of pregnancy, when they rose to approximately 15 pg/ml. The infusion of 130 μg/h purified 2-OHE2 elevated its plasma concentration to 155 pg/ml, consistent with a plasma MCR (MCRp) of approximately 20, 000 liters/day. The infusion of [3H] 2-OHE2 to equilibrium and chromatographic separation of the extracted plasma metabolites yielded an MCRp of about 13, 000 liters/day; the major plasma metabolite comigrated with 2-methoxyestradiol, and [3H]/-methoxyestrone was also formed. The MCRp of 2-OHE2 is approximately half that of 2-hydroxyestrone (2-0HE), but much higher than those of other steroids. As is true for 2-OHE1, the clearance of 2-OHE2 must occur primarily in the blood compartment. Together, the measured MCRP values and estrogen receptor affinities of 2-OHE2 and 2-OHE 1 predict a relative potency for effects upon gonadotropin secretion which i close to that observed in vivo.

Original languageEnglish (US)
Pages (from-to)173-176
Number of pages4
JournalJournal of Clinical Endocrinology and Metabolism
Volume54
Issue number1
DOIs
StatePublished - 1982
Externally publishedYes

Fingerprint

Catechol Estrogens
Metabolism
Radioimmunoassay
Plasmas
Metabolites
Estrogen Receptor beta
Third Pregnancy Trimester
Gonadotropins
Estrogen Receptors
Limit of Detection
Assays
Blood
Steroids
2-hydroxyestradiol

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Biochemistry
  • Endocrinology
  • Clinical Biochemistry
  • Biochemistry, medical

Cite this

Radioimmunoassay and metabolism of the catechol estrogen 2-hydroxyestradiol. / Kono, Shinzo; Merriam, George R.; Brandon, David D.; Loriaux, Donald (Lynn); Lipsett, Mortimer B.

In: Journal of Clinical Endocrinology and Metabolism, Vol. 54, No. 1, 1982, p. 173-176.

Research output: Contribution to journalArticle

Kono, Shinzo ; Merriam, George R. ; Brandon, David D. ; Loriaux, Donald (Lynn) ; Lipsett, Mortimer B. / Radioimmunoassay and metabolism of the catechol estrogen 2-hydroxyestradiol. In: Journal of Clinical Endocrinology and Metabolism. 1982 ; Vol. 54, No. 1. pp. 173-176.
@article{5ded2c42aaf7490081ee52e7b2c2bc53,
title = "Radioimmunoassay and metabolism of the catechol estrogen 2-hydroxyestradiol",
abstract = "Plasma levels of 2-hydroxyestradiol (2-OHE2)were measured using a new RIA procedure. Values were below the detection limit of the assay (<10 pg/ml), except in the third trimester of pregnancy, when they rose to approximately 15 pg/ml. The infusion of 130 μg/h purified 2-OHE2 elevated its plasma concentration to 155 pg/ml, consistent with a plasma MCR (MCRp) of approximately 20, 000 liters/day. The infusion of [3H] 2-OHE2 to equilibrium and chromatographic separation of the extracted plasma metabolites yielded an MCRp of about 13, 000 liters/day; the major plasma metabolite comigrated with 2-methoxyestradiol, and [3H]/-methoxyestrone was also formed. The MCRp of 2-OHE2 is approximately half that of 2-hydroxyestrone (2-0HE), but much higher than those of other steroids. As is true for 2-OHE1, the clearance of 2-OHE2 must occur primarily in the blood compartment. Together, the measured MCRP values and estrogen receptor affinities of 2-OHE2 and 2-OHE 1 predict a relative potency for effects upon gonadotropin secretion which i close to that observed in vivo.",
author = "Shinzo Kono and Merriam, {George R.} and Brandon, {David D.} and Loriaux, {Donald (Lynn)} and Lipsett, {Mortimer B.}",
year = "1982",
doi = "10.1210/jcem-54-1-150",
language = "English (US)",
volume = "54",
pages = "173--176",
journal = "Journal of Clinical Endocrinology and Metabolism",
issn = "0021-972X",
publisher = "The Endocrine Society",
number = "1",

}

TY - JOUR

T1 - Radioimmunoassay and metabolism of the catechol estrogen 2-hydroxyestradiol

AU - Kono, Shinzo

AU - Merriam, George R.

AU - Brandon, David D.

AU - Loriaux, Donald (Lynn)

AU - Lipsett, Mortimer B.

PY - 1982

Y1 - 1982

N2 - Plasma levels of 2-hydroxyestradiol (2-OHE2)were measured using a new RIA procedure. Values were below the detection limit of the assay (<10 pg/ml), except in the third trimester of pregnancy, when they rose to approximately 15 pg/ml. The infusion of 130 μg/h purified 2-OHE2 elevated its plasma concentration to 155 pg/ml, consistent with a plasma MCR (MCRp) of approximately 20, 000 liters/day. The infusion of [3H] 2-OHE2 to equilibrium and chromatographic separation of the extracted plasma metabolites yielded an MCRp of about 13, 000 liters/day; the major plasma metabolite comigrated with 2-methoxyestradiol, and [3H]/-methoxyestrone was also formed. The MCRp of 2-OHE2 is approximately half that of 2-hydroxyestrone (2-0HE), but much higher than those of other steroids. As is true for 2-OHE1, the clearance of 2-OHE2 must occur primarily in the blood compartment. Together, the measured MCRP values and estrogen receptor affinities of 2-OHE2 and 2-OHE 1 predict a relative potency for effects upon gonadotropin secretion which i close to that observed in vivo.

AB - Plasma levels of 2-hydroxyestradiol (2-OHE2)were measured using a new RIA procedure. Values were below the detection limit of the assay (<10 pg/ml), except in the third trimester of pregnancy, when they rose to approximately 15 pg/ml. The infusion of 130 μg/h purified 2-OHE2 elevated its plasma concentration to 155 pg/ml, consistent with a plasma MCR (MCRp) of approximately 20, 000 liters/day. The infusion of [3H] 2-OHE2 to equilibrium and chromatographic separation of the extracted plasma metabolites yielded an MCRp of about 13, 000 liters/day; the major plasma metabolite comigrated with 2-methoxyestradiol, and [3H]/-methoxyestrone was also formed. The MCRp of 2-OHE2 is approximately half that of 2-hydroxyestrone (2-0HE), but much higher than those of other steroids. As is true for 2-OHE1, the clearance of 2-OHE2 must occur primarily in the blood compartment. Together, the measured MCRP values and estrogen receptor affinities of 2-OHE2 and 2-OHE 1 predict a relative potency for effects upon gonadotropin secretion which i close to that observed in vivo.

UR - http://www.scopus.com/inward/record.url?scp=85003188099&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85003188099&partnerID=8YFLogxK

U2 - 10.1210/jcem-54-1-150

DO - 10.1210/jcem-54-1-150

M3 - Article

AN - SCOPUS:85003188099

VL - 54

SP - 173

EP - 176

JO - Journal of Clinical Endocrinology and Metabolism

JF - Journal of Clinical Endocrinology and Metabolism

SN - 0021-972X

IS - 1

ER -