Radiation pretreatment does not protect the rat optic nerve from elevated intraocular pressure–induced injury

Elaine C. Johnson, William O. Cepurna, Dongseok Choi, Tiffany E. Choe, John C. Morrison

Research output: Contribution to journalArticle

13 Scopus citations

Abstract

Methods. Episcleral vein injection of hypertonic saline was used to elevate IOP unilaterally in two groups of rats: (1) otherwise untreated and (2) radiation pretreated, n> 25/group. Intraocular pressure histories were collected for 5 weeks, when optic nerves were prepared and graded for injury. Statistical analyses were used to compare IOP history and nerve injury. The density of microglia and macrophages in two nerve head regions was determined by Iba1 immunolabeling.

Results. Mean and peak IOP elevations were not different between the two glaucoma model groups. Mean optic nerve injury grades were not different in glaucoma model optic nerves and were equivalent to approximately 35% of axons degenerating. Nerves selected for lower mean or peak IOP elevations did not differ in optic nerve injury. Similarly, nerves selected for lower injury grade did not differ in IOP exposure. By multiple regression modeling, nerve injury grade was most significantly associated with mean IOP (P <0.002). There was no significant effect of radiation treatment. Iba1þ cell density was not altered by radiation treatment.

Conclusions:. In contrast to previous observations in a mouse genetic glaucoma model, headonly irradiation offers the adult rat optic nerve no protection from optic nerve degeneration due to chronic, experimentally induced IOP elevation.

Purpose. Optic nerve injury has been found to be dramatically reduced in a genetic mouse glaucoma model following exposure to sublethal, head-only irradiation. In this study, the same radiation treatment was used prior to experimental induction of elevated intraocular pressure (IOP) to determine if radiation is neuroprotective in another glaucoma model.

Original languageEnglish (US)
Pages (from-to)412-419
Number of pages8
JournalInvestigative Ophthalmology and Visual Science
Volume56
Issue number1
DOIs
StatePublished - Dec 7 2014

Keywords

  • Animal models
  • Axon degeneration
  • Glaucoma
  • Intraocular pressure
  • Optic nerve
  • Radiation

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

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