Radiation-induced chromosome aberrations analysed by fluorescence in situ hybridization with a triple combination of composite whole chromosome-specific DNA probes

E. Schmid; H. Zitzelsberger; H. Braselmann; J. W. Gray; M. Bauchinger

doi:10.1080/09553009214552621

Radiation-induced chromosome aberrations analysed by fluorescence in situ hybridization with a triple combination of composite whole chromosome-specific DNA probes

E. Schmid, H. Zitzelsberger, H. Braselmann, J. W. Gray, M. Bauchinger

Research output: Contribution to journal › Article › peer-review

133 Scopus citations

Abstract

Fluorescence in situ hybridization (FISH) with a combination of three composite whole chromosome-specific DNA probes for human chromosomes 1, 4 and 12 was used to analyse in vitro radiation-induced dicentrics and symmetrical translocations in peripheral lymphocytes. Translocations could be rapidly and efficiently detected by FISH. Their frequencies were 1.8-fold higher than the frequencies for dicentrics at a given dose. The dose-response curves for translocations and dicentrics were linear quadratic with a significant higher quadratic component for translocations. The application of FISH for scoring stable translocations for biological dosimetry of radiation exposures is discussed.

Original language	English (US)
Pages (from-to)	673-678
Number of pages	6
Journal	International Journal of Radiation Biology
Volume	62
Issue number	6
DOIs	https://doi.org/10.1080/09553009214552621
State	Published - 1992
Externally published	Yes

ASJC Scopus subject areas

Radiological and Ultrasound Technology
Radiology Nuclear Medicine and imaging

Access to Document

10.1080/09553009214552621

Cite this

@article{814bd26723b5495bbc7c3c255d18dbd3,

title = "Radiation-induced chromosome aberrations analysed by fluorescence in situ hybridization with a triple combination of composite whole chromosome-specific DNA probes",

abstract = "Fluorescence in situ hybridization (FISH) with a combination of three composite whole chromosome-specific DNA probes for human chromosomes 1, 4 and 12 was used to analyse in vitro radiation-induced dicentrics and symmetrical translocations in peripheral lymphocytes. Translocations could be rapidly and efficiently detected by FISH. Their frequencies were 1.8-fold higher than the frequencies for dicentrics at a given dose. The dose-response curves for translocations and dicentrics were linear quadratic with a significant higher quadratic component for translocations. The application of FISH for scoring stable translocations for biological dosimetry of radiation exposures is discussed.",

author = "E. Schmid and H. Zitzelsberger and H. Braselmann and Gray, {J. W.} and M. Bauchinger",

year = "1992",

doi = "10.1080/09553009214552621",

language = "English (US)",

volume = "62",

pages = "673--678",

journal = "International Journal of Radiation Biology",

issn = "0955-3002",

publisher = "Informa Healthcare",

number = "6",

}

TY - JOUR

T1 - Radiation-induced chromosome aberrations analysed by fluorescence in situ hybridization with a triple combination of composite whole chromosome-specific DNA probes

AU - Schmid, E.

AU - Zitzelsberger, H.

AU - Braselmann, H.

AU - Gray, J. W.

AU - Bauchinger, M.

PY - 1992

Y1 - 1992

N2 - Fluorescence in situ hybridization (FISH) with a combination of three composite whole chromosome-specific DNA probes for human chromosomes 1, 4 and 12 was used to analyse in vitro radiation-induced dicentrics and symmetrical translocations in peripheral lymphocytes. Translocations could be rapidly and efficiently detected by FISH. Their frequencies were 1.8-fold higher than the frequencies for dicentrics at a given dose. The dose-response curves for translocations and dicentrics were linear quadratic with a significant higher quadratic component for translocations. The application of FISH for scoring stable translocations for biological dosimetry of radiation exposures is discussed.

AB - Fluorescence in situ hybridization (FISH) with a combination of three composite whole chromosome-specific DNA probes for human chromosomes 1, 4 and 12 was used to analyse in vitro radiation-induced dicentrics and symmetrical translocations in peripheral lymphocytes. Translocations could be rapidly and efficiently detected by FISH. Their frequencies were 1.8-fold higher than the frequencies for dicentrics at a given dose. The dose-response curves for translocations and dicentrics were linear quadratic with a significant higher quadratic component for translocations. The application of FISH for scoring stable translocations for biological dosimetry of radiation exposures is discussed.

UR - http://www.scopus.com/inward/record.url?scp=0027048947&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027048947&partnerID=8YFLogxK

U2 - 10.1080/09553009214552621

DO - 10.1080/09553009214552621

M3 - Article

C2 - 1362760

AN - SCOPUS:0027048947

SN - 0955-3002

VL - 62

SP - 673

EP - 678

JO - International Journal of Radiation Biology

JF - International Journal of Radiation Biology

IS - 6

ER -

Radiation-induced chromosome aberrations analysed by fluorescence in situ hybridization with a triple combination of composite whole chromosome-specific DNA probes

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this