Radiation-induced chromosome aberrations analysed by fluorescence in situ hybridization with a triple combination of composite whole chromosome-specific DNA probes

E. Schmid, H. Zitzelsberger, H. Braselmann, J. W. Gray, M. Bauchinger

Research output: Contribution to journalArticlepeer-review

131 Scopus citations

Abstract

Fluorescence in situ hybridization (FISH) with a combination of three composite whole chromosome-specific DNA probes for human chromosomes 1, 4 and 12 was used to analyse in vitro radiation-induced dicentrics and symmetrical translocations in peripheral lymphocytes. Translocations could be rapidly and efficiently detected by FISH. Their frequencies were 1.8-fold higher than the frequencies for dicentrics at a given dose. The dose-response curves for translocations and dicentrics were linear quadratic with a significant higher quadratic component for translocations. The application of FISH for scoring stable translocations for biological dosimetry of radiation exposures is discussed.

Original languageEnglish (US)
Pages (from-to)673-678
Number of pages6
JournalInternational Journal of Radiation Biology
Volume62
Issue number6
DOIs
StatePublished - Jan 1 1992

ASJC Scopus subject areas

  • Radiological and Ultrasound Technology
  • Radiology Nuclear Medicine and imaging

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