Radiation-induced chromosome aberrations analysed by fluorescence in situ hybridization with a triple combination of composite whole chromosome-specific DNA probes

E. Schmid, H. Zitzelsberger, H. Braselmann, Joe Gray, M. Bauchinger

Research output: Contribution to journalArticle

131 Citations (Scopus)

Abstract

Fluorescence in situ hybridization (FISH) with a combination of three composite whole chromosome-specific DNA probes for human chromosomes 1, 4 and 12 was used to analyse in vitro radiation-induced dicentrics and symmetrical translocations in peripheral lymphocytes. Translocations could be rapidly and efficiently detected by FISH. Their frequencies were 1.8-fold higher than the frequencies for dicentrics at a given dose. The dose-response curves for translocations and dicentrics were linear quadratic with a significant higher quadratic component for translocations. The application of FISH for scoring stable translocations for biological dosimetry of radiation exposures is discussed.

Original languageEnglish (US)
Pages (from-to)673-678
Number of pages6
JournalInternational Journal of Radiation Biology
Volume62
Issue number6
DOIs
StatePublished - 1992
Externally publishedYes

Fingerprint

chromosome aberrations
chromosomes
DNA probes
DNA Probes
Chromosomes
fluorescence in situ hybridization
Aberrations
Fluorescence In Situ Hybridization
Chromosome Aberrations
DNA
deoxyribonucleic acid
Fluorescence
Radiation
fluorescence
composite materials
probes
Composite materials
radiation
Chromosomes, Human, Pair 12
dosage

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging
  • Radiological and Ultrasound Technology
  • Agricultural and Biological Sciences (miscellaneous)
  • Nuclear Energy and Engineering
  • Radiation

Cite this

Radiation-induced chromosome aberrations analysed by fluorescence in situ hybridization with a triple combination of composite whole chromosome-specific DNA probes. / Schmid, E.; Zitzelsberger, H.; Braselmann, H.; Gray, Joe; Bauchinger, M.

In: International Journal of Radiation Biology, Vol. 62, No. 6, 1992, p. 673-678.

Research output: Contribution to journalArticle

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AU - Bauchinger, M.

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