Rac1 is essential for platelet lamellipodia formation and aggregate stability under flow

Owen McCarty, Mark K. Larson, Jocelyn M. Auger, Neena Kalia, Ben T. Atkinson, Andrew C. Pearce, Sandra Ruf, Robert B. Henderson, Victor L J Tybulewicz, Laura M. Machesky, Steve P. Watson

Research output: Contribution to journalArticle

154 Citations (Scopus)

Abstract

The role of Rac family proteins in platelet spreading on matrix proteins under static and flow conditions has been investigated by using Rac-deficient platelets. Murine platelets form filopodia and undergo limited spreading on fibrinogen independent of Rac1 and Rac2. In the presence of thrombin, marked lamellipodia formation is observed on fibrinogen, which is abrogated in the absence of Rac1. However, Rac1 is not required for thrombin-induced aggregation or elevation of F-actin levels. Formation of lamellipodia on collagen and laminin is also Rac1-dependent. Analysis of platelet adhesion dynamics on collagen under flow conditions in vitro revealed that Rac1 is required for platelet aggregate stability at arterial rates of shear, as evidenced by a dramatic increase in platelet embolization. Furthermore, studies employing intravital microscopy demonstrated that Rac1 plays a critical role in the development of stable thrombi at sites of vascular injury in vivo. Thus, our data demonstrated that Rac1 is essential for lamellipodia formation in platelets and indicated that Rac1 is required for aggregate integrity leading to thrombus formation under physiologically relevant levels of shear both in vitro and in vivo.

Original languageEnglish (US)
Pages (from-to)39474-39484
Number of pages11
JournalJournal of Biological Chemistry
Volume280
Issue number47
DOIs
StatePublished - Nov 25 2005

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Pseudopodia
Platelets
Blood Platelets
Thrombin
Fibrinogen
Thrombosis
rac GTP-Binding Proteins
Collagen
Vascular System Injuries
Laminin
Actins
Proteins
Adhesion
Agglomeration

ASJC Scopus subject areas

  • Biochemistry

Cite this

McCarty, O., Larson, M. K., Auger, J. M., Kalia, N., Atkinson, B. T., Pearce, A. C., ... Watson, S. P. (2005). Rac1 is essential for platelet lamellipodia formation and aggregate stability under flow. Journal of Biological Chemistry, 280(47), 39474-39484. https://doi.org/10.1074/jbc.M504672200

Rac1 is essential for platelet lamellipodia formation and aggregate stability under flow. / McCarty, Owen; Larson, Mark K.; Auger, Jocelyn M.; Kalia, Neena; Atkinson, Ben T.; Pearce, Andrew C.; Ruf, Sandra; Henderson, Robert B.; Tybulewicz, Victor L J; Machesky, Laura M.; Watson, Steve P.

In: Journal of Biological Chemistry, Vol. 280, No. 47, 25.11.2005, p. 39474-39484.

Research output: Contribution to journalArticle

McCarty, O, Larson, MK, Auger, JM, Kalia, N, Atkinson, BT, Pearce, AC, Ruf, S, Henderson, RB, Tybulewicz, VLJ, Machesky, LM & Watson, SP 2005, 'Rac1 is essential for platelet lamellipodia formation and aggregate stability under flow', Journal of Biological Chemistry, vol. 280, no. 47, pp. 39474-39484. https://doi.org/10.1074/jbc.M504672200
McCarty, Owen ; Larson, Mark K. ; Auger, Jocelyn M. ; Kalia, Neena ; Atkinson, Ben T. ; Pearce, Andrew C. ; Ruf, Sandra ; Henderson, Robert B. ; Tybulewicz, Victor L J ; Machesky, Laura M. ; Watson, Steve P. / Rac1 is essential for platelet lamellipodia formation and aggregate stability under flow. In: Journal of Biological Chemistry. 2005 ; Vol. 280, No. 47. pp. 39474-39484.
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