Rabbit model of disseminated syphilis: Immunoblot and immunohistologic evidence for a role of specific immune complexes in lesion pathogenesis

J. L. Jorizzo, M. C. McNeely, R. E. Baughn, T. Cavallo, Alvin Jr Solomon, E. B. Smith

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Circulating immune complexes (CIC) containing Treponema pallidum proteins have been preliminary implicated as inducers of a neutrophilic vascular reaction in early human cutaneouos lesions of secondary syphilis. To clarify the role of specific CIC in producing cutaneous and renal lesions, 12 rabbits were studied at the following intervals after induction of disseminated syphilis: 20 days (4 rabbits: biopsies of normal and lesional skin for direct immunofluorescence (IMF) for (IgG, IgM, IgA, Clq, C3, C4), fibrin, and T. pallidum proteins; routine histology; and immunoblots of serum for CIC containing T. pallidum proteins); 21 days (4 rabbits: as at 20 days without IMF for T. pallidum protein); 23 days (4 rabbits: as at 20 days without IMF); 30 days (same 12 rabbits restudied with routine histology of normal and lesional skin; kidneys from 4 rabbits removed for routine, IMF, and electron microscopy (EM). Treponemal polypeptide antigen (MW-87kd) was demonstrated in CIC from rabbits. Routine cutaneous histology showed evolution of lesions from an early neutrophilic vascular reaction to the typical lymphoplasmacytic reaction. IMF showed vessel-based immunoreactants in 3 of the 4 rabbits tested at 20 days and 1 of 4 at 21 days, and T. pallidum proteins in 3 of 4 rabbits at 20 days. Routine histology, IMF, and EM studies of glomeruli showed glomerular abnormalities, but no evidence of immune deposits containing specific T. pallidum protein. Skin and kidney studies of 4 controls were all negative. These data indicate a role for specific immune complexes in the pathogenesis of cutaneous lesions in this rabbit model.

Original languageEnglish (US)
Pages (from-to)150-160
Number of pages11
JournalJournal of Cutaneous Pathology
Volume15
Issue number3
StatePublished - 1988
Externally publishedYes

Fingerprint

Syphilis
Antigen-Antibody Complex
Treponema pallidum
Rabbits
Skin
Histology
Fluorescent Antibody Technique
Proteins
Kidney
Fluorescence Microscopy
Blood Vessels
Electron Microscopy
Direct Fluorescent Antibody Technique
Fibrin
Immunoglobulin A
Immunoglobulin M
Immunoglobulin G
Biopsy
Antigens
Peptides

ASJC Scopus subject areas

  • Dermatology
  • Pathology and Forensic Medicine

Cite this

Jorizzo, J. L., McNeely, M. C., Baughn, R. E., Cavallo, T., Solomon, A. J., & Smith, E. B. (1988). Rabbit model of disseminated syphilis: Immunoblot and immunohistologic evidence for a role of specific immune complexes in lesion pathogenesis. Journal of Cutaneous Pathology, 15(3), 150-160.

Rabbit model of disseminated syphilis : Immunoblot and immunohistologic evidence for a role of specific immune complexes in lesion pathogenesis. / Jorizzo, J. L.; McNeely, M. C.; Baughn, R. E.; Cavallo, T.; Solomon, Alvin Jr; Smith, E. B.

In: Journal of Cutaneous Pathology, Vol. 15, No. 3, 1988, p. 150-160.

Research output: Contribution to journalArticle

Jorizzo, J. L. ; McNeely, M. C. ; Baughn, R. E. ; Cavallo, T. ; Solomon, Alvin Jr ; Smith, E. B. / Rabbit model of disseminated syphilis : Immunoblot and immunohistologic evidence for a role of specific immune complexes in lesion pathogenesis. In: Journal of Cutaneous Pathology. 1988 ; Vol. 15, No. 3. pp. 150-160.
@article{b847a536740b4d40ace5226458429140,
title = "Rabbit model of disseminated syphilis: Immunoblot and immunohistologic evidence for a role of specific immune complexes in lesion pathogenesis",
abstract = "Circulating immune complexes (CIC) containing Treponema pallidum proteins have been preliminary implicated as inducers of a neutrophilic vascular reaction in early human cutaneouos lesions of secondary syphilis. To clarify the role of specific CIC in producing cutaneous and renal lesions, 12 rabbits were studied at the following intervals after induction of disseminated syphilis: 20 days (4 rabbits: biopsies of normal and lesional skin for direct immunofluorescence (IMF) for (IgG, IgM, IgA, Clq, C3, C4), fibrin, and T. pallidum proteins; routine histology; and immunoblots of serum for CIC containing T. pallidum proteins); 21 days (4 rabbits: as at 20 days without IMF for T. pallidum protein); 23 days (4 rabbits: as at 20 days without IMF); 30 days (same 12 rabbits restudied with routine histology of normal and lesional skin; kidneys from 4 rabbits removed for routine, IMF, and electron microscopy (EM). Treponemal polypeptide antigen (MW-87kd) was demonstrated in CIC from rabbits. Routine cutaneous histology showed evolution of lesions from an early neutrophilic vascular reaction to the typical lymphoplasmacytic reaction. IMF showed vessel-based immunoreactants in 3 of the 4 rabbits tested at 20 days and 1 of 4 at 21 days, and T. pallidum proteins in 3 of 4 rabbits at 20 days. Routine histology, IMF, and EM studies of glomeruli showed glomerular abnormalities, but no evidence of immune deposits containing specific T. pallidum protein. Skin and kidney studies of 4 controls were all negative. These data indicate a role for specific immune complexes in the pathogenesis of cutaneous lesions in this rabbit model.",
author = "Jorizzo, {J. L.} and McNeely, {M. C.} and Baughn, {R. E.} and T. Cavallo and Solomon, {Alvin Jr} and Smith, {E. B.}",
year = "1988",
language = "English (US)",
volume = "15",
pages = "150--160",
journal = "Journal of Cutaneous Pathology",
issn = "0303-6987",
publisher = "Wiley-Blackwell",
number = "3",

}

TY - JOUR

T1 - Rabbit model of disseminated syphilis

T2 - Immunoblot and immunohistologic evidence for a role of specific immune complexes in lesion pathogenesis

AU - Jorizzo, J. L.

AU - McNeely, M. C.

AU - Baughn, R. E.

AU - Cavallo, T.

AU - Solomon, Alvin Jr

AU - Smith, E. B.

PY - 1988

Y1 - 1988

N2 - Circulating immune complexes (CIC) containing Treponema pallidum proteins have been preliminary implicated as inducers of a neutrophilic vascular reaction in early human cutaneouos lesions of secondary syphilis. To clarify the role of specific CIC in producing cutaneous and renal lesions, 12 rabbits were studied at the following intervals after induction of disseminated syphilis: 20 days (4 rabbits: biopsies of normal and lesional skin for direct immunofluorescence (IMF) for (IgG, IgM, IgA, Clq, C3, C4), fibrin, and T. pallidum proteins; routine histology; and immunoblots of serum for CIC containing T. pallidum proteins); 21 days (4 rabbits: as at 20 days without IMF for T. pallidum protein); 23 days (4 rabbits: as at 20 days without IMF); 30 days (same 12 rabbits restudied with routine histology of normal and lesional skin; kidneys from 4 rabbits removed for routine, IMF, and electron microscopy (EM). Treponemal polypeptide antigen (MW-87kd) was demonstrated in CIC from rabbits. Routine cutaneous histology showed evolution of lesions from an early neutrophilic vascular reaction to the typical lymphoplasmacytic reaction. IMF showed vessel-based immunoreactants in 3 of the 4 rabbits tested at 20 days and 1 of 4 at 21 days, and T. pallidum proteins in 3 of 4 rabbits at 20 days. Routine histology, IMF, and EM studies of glomeruli showed glomerular abnormalities, but no evidence of immune deposits containing specific T. pallidum protein. Skin and kidney studies of 4 controls were all negative. These data indicate a role for specific immune complexes in the pathogenesis of cutaneous lesions in this rabbit model.

AB - Circulating immune complexes (CIC) containing Treponema pallidum proteins have been preliminary implicated as inducers of a neutrophilic vascular reaction in early human cutaneouos lesions of secondary syphilis. To clarify the role of specific CIC in producing cutaneous and renal lesions, 12 rabbits were studied at the following intervals after induction of disseminated syphilis: 20 days (4 rabbits: biopsies of normal and lesional skin for direct immunofluorescence (IMF) for (IgG, IgM, IgA, Clq, C3, C4), fibrin, and T. pallidum proteins; routine histology; and immunoblots of serum for CIC containing T. pallidum proteins); 21 days (4 rabbits: as at 20 days without IMF for T. pallidum protein); 23 days (4 rabbits: as at 20 days without IMF); 30 days (same 12 rabbits restudied with routine histology of normal and lesional skin; kidneys from 4 rabbits removed for routine, IMF, and electron microscopy (EM). Treponemal polypeptide antigen (MW-87kd) was demonstrated in CIC from rabbits. Routine cutaneous histology showed evolution of lesions from an early neutrophilic vascular reaction to the typical lymphoplasmacytic reaction. IMF showed vessel-based immunoreactants in 3 of the 4 rabbits tested at 20 days and 1 of 4 at 21 days, and T. pallidum proteins in 3 of 4 rabbits at 20 days. Routine histology, IMF, and EM studies of glomeruli showed glomerular abnormalities, but no evidence of immune deposits containing specific T. pallidum protein. Skin and kidney studies of 4 controls were all negative. These data indicate a role for specific immune complexes in the pathogenesis of cutaneous lesions in this rabbit model.

UR - http://www.scopus.com/inward/record.url?scp=0023925196&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023925196&partnerID=8YFLogxK

M3 - Article

C2 - 3294256

AN - SCOPUS:0023925196

VL - 15

SP - 150

EP - 160

JO - Journal of Cutaneous Pathology

JF - Journal of Cutaneous Pathology

SN - 0303-6987

IS - 3

ER -