Quiescence and activation of stem and precursor cell populations in the subependymal zone of the mammalian brain are associated with distinct cellular and extracellular matrix signals

Ilias Kazanis, Justin D. Lathia, Tegy J. Vadakkan, Eric Raborn, Ruiqian Wan, Mohamed R. Mughal, D. Mark Eckley, Takako Sasaki, Bruce Patton, Mark P. Mattson, Karen K. Hirschi, Mary E. Dickinson, Charles Ffrench-Constant

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Abstract

The subependymal zone (SEZ) of the lateral ventricles is one of the areas of the adult brain where new neurons are continuously generated from neural stem cells (NSCs), via rapidly dividing precursors. This neurogenic niche is a complex cellular and extracellular microenvironment, highly vascularized compared to non-neurogenic periventricular areas, within which NSCs and precursors exhibit distinct behavior. Here, we investigate the possible mechanisms by which extracellular matrix molecules and their receptors might regulate this differential behavior. We show that NSCs and precursors proceed through mitosis in the same domains within the SEZ of adult male mice - albeit with NSCs nearer ependymal cells - and that distance from the ventricle is a stronger limiting factor for neurogenic activity than distance from blood vessels. Furthermore, we show that NSCs and precursors are embedded in a laminin-rich extracellular matrix, to which they can both contribute. Importantly, they express differential levels of extracellular matrix receptors, with NSCs expressing low levels of α6β1 integrin, syndecan-1, and lutheran, and in vivo blocking of β1 integrin selectively induced the proliferation and ectopic migration of precursors. Finally, when NSCs are activated to reconstitute the niche after depletion of precursors, expression of laminin receptors is upregulated. These results indicate that the distinct behavior of adult NSCs and precursors is not necessarily regulated via exposure to differential extracellular signals, but rather via intrinsic regulation of their interaction with their microenvironment. Copyright

Original languageEnglish (US)
Pages (from-to)9771-9781
Number of pages11
JournalJournal of Neuroscience
Volume30
Issue number29
DOIs
StatePublished - Jul 21 2010

ASJC Scopus subject areas

  • Neuroscience(all)

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    Kazanis, I., Lathia, J. D., Vadakkan, T. J., Raborn, E., Wan, R., Mughal, M. R., Eckley, D. M., Sasaki, T., Patton, B., Mattson, M. P., Hirschi, K. K., Dickinson, M. E., & Ffrench-Constant, C. (2010). Quiescence and activation of stem and precursor cell populations in the subependymal zone of the mammalian brain are associated with distinct cellular and extracellular matrix signals. Journal of Neuroscience, 30(29), 9771-9781. https://doi.org/10.1523/JNEUROSCI.0700-10.2010