Quantum dot mediated imaging of atherosclerosis

Ashwath Jayagopal, Yan Ru Su, John L. Blakemore, MacRae F. Linton, Sergio Fazio, Frederick R. Haselton

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

The progression of atherosclerosis is associated with leukocyte infiltration within lesions. We describe a technique for the ex vivo imaging of cellular recruitment in atherogenesis which utilizes quantum dots (QD) to color-code different cell types within lesion areas. Spectrally distinct QD were coated with the cell-penetrating peptide maurocalcine to fluorescently-label immunomagnetically isolated monocyte/macrophages and T lymphocytes. QD-maurocalcine bioconjugates labeled both cell types with a high efficiency, preserved cell viability, and did not perturb native leukocyte function in cytokine release and endothelial adhesion assays. QD-labeled monocyte/macrophages and T lymphocytes were reinfused in an ApoE-/- mouse model of atherosclerosis and age-matched controls and tracked for up to four weeks to investigate the incorporation of cells within aortic lesion areas, as determined by oil red O (ORO) and immunofluorescence ex vivo staining. QD-labeled cells were visible in atherosclerotic plaques within two days of injection, and the two cell types colocalized within areas of subsequent ORO staining. Our method for tracking leukocytes in lesions enables high signal-to-noise ratio imaging of multiple cell types and biomarkers simultaneously within the same specimen. It also has great utility in studies aimed at investigating the role of distinct circulating leukocyte subsets in plaque development and progression.

Original languageEnglish (US)
Article number165102
JournalNanotechnology
Volume20
Issue number16
DOIs
StatePublished - 2009
Externally publishedYes

Fingerprint

Semiconductor quantum dots
Imaging techniques
T-cells
Macrophages
Color codes
Cell-Penetrating Peptides
Apolipoproteins E
Biomarkers
Infiltration
Peptides
Labels
Assays
Signal to noise ratio
Adhesion
Cells
Cytokines
maurocalcine
oil red O
Oils

ASJC Scopus subject areas

  • Bioengineering
  • Chemistry(all)
  • Electrical and Electronic Engineering
  • Mechanical Engineering
  • Mechanics of Materials
  • Materials Science(all)

Cite this

Jayagopal, A., Su, Y. R., Blakemore, J. L., Linton, M. F., Fazio, S., & Haselton, F. R. (2009). Quantum dot mediated imaging of atherosclerosis. Nanotechnology, 20(16), [165102]. https://doi.org/10.1088/0957-4484/20/16/165102

Quantum dot mediated imaging of atherosclerosis. / Jayagopal, Ashwath; Su, Yan Ru; Blakemore, John L.; Linton, MacRae F.; Fazio, Sergio; Haselton, Frederick R.

In: Nanotechnology, Vol. 20, No. 16, 165102, 2009.

Research output: Contribution to journalArticle

Jayagopal, A, Su, YR, Blakemore, JL, Linton, MF, Fazio, S & Haselton, FR 2009, 'Quantum dot mediated imaging of atherosclerosis', Nanotechnology, vol. 20, no. 16, 165102. https://doi.org/10.1088/0957-4484/20/16/165102
Jayagopal A, Su YR, Blakemore JL, Linton MF, Fazio S, Haselton FR. Quantum dot mediated imaging of atherosclerosis. Nanotechnology. 2009;20(16). 165102. https://doi.org/10.1088/0957-4484/20/16/165102
Jayagopal, Ashwath ; Su, Yan Ru ; Blakemore, John L. ; Linton, MacRae F. ; Fazio, Sergio ; Haselton, Frederick R. / Quantum dot mediated imaging of atherosclerosis. In: Nanotechnology. 2009 ; Vol. 20, No. 16.
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