Quantitative proteomics of the thyroid hormone receptor-coregulator interactions

Jamie M R Moore, Sarah J. Galicia, Andrea C. McReynolds, Ngoc Ha Nguyen, Thomas (Tom) Scanlan, R. Kiplin Guy

Research output: Contribution to journalArticle

53 Citations (Scopus)

Abstract

The thyroid hormone receptor regulates a diverse set of genes that control processes from embryonic development to adult homeostasis. Upon binding of thyroid hormone, the thyroid receptor releases corepressor proteins and undergoes a conformational change that allows for the interaction of coactivating proteins necessary for gene transcription. This interaction is mediated by a conserved motif, termed the NR box, found in many coregulators. Recent work has demonstrated that differentially assembled coregulator complexes can elicit specific biological responses. However, the mechanism for the selective assembly of these coregulator complexes has yet to be elucidated. To further understand the principles underlying thyroid receptor-coregulator selectivity, we designed a high-throughput in vitro binding assay to measure the equilibrium affinity of thyroid receptor to a library of potential coregulators in the presence of different ligands including the endogenous thyroid hormone T3, synthetic thyroid receptor β-selective agonist GC-1, and antagonist NH-3. Using this homogenous method several coregulator NR boxes capable of associating with thyroid receptor at physiologically relevant concentrations were identified including ones found in traditional coactivating proteins such as SRC1, SRC2, TRAP220, TRBP, p300, and ARA70; and those in coregulators known to repress gene activation including RIP140 and DAX-1. In addition, it was discovered that the thyroid receptor-coregulator binding patterns vary with ligand and that this differential binding can be used to predict biological responses. Finally, it is demonstrated that this is a general method that can be applied to other nuclear receptors and can be used to establish rules for nuclear receptor-coregulator selectivity.

Original languageEnglish (US)
Pages (from-to)27584-27590
Number of pages7
JournalJournal of Biological Chemistry
Volume279
Issue number26
DOIs
StatePublished - Jun 25 2004
Externally publishedYes

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Thyroid Hormone Receptors
Proteomics
Thyroid Gland
Genes
Cytoplasmic and Nuclear Receptors
Mediator Complex Subunit 1
Ligands
Co-Repressor Proteins
Triiodothyronine
Transcription
Thyroid Hormones
Assays
Proteins
Chemical activation
Artificial Receptors
Throughput
Transcriptional Activation
Libraries
Embryonic Development
Homeostasis

ASJC Scopus subject areas

  • Biochemistry

Cite this

Moore, J. M. R., Galicia, S. J., McReynolds, A. C., Nguyen, N. H., Scanlan, T. T., & Guy, R. K. (2004). Quantitative proteomics of the thyroid hormone receptor-coregulator interactions. Journal of Biological Chemistry, 279(26), 27584-27590. https://doi.org/10.1074/jbc.M403453200

Quantitative proteomics of the thyroid hormone receptor-coregulator interactions. / Moore, Jamie M R; Galicia, Sarah J.; McReynolds, Andrea C.; Nguyen, Ngoc Ha; Scanlan, Thomas (Tom); Guy, R. Kiplin.

In: Journal of Biological Chemistry, Vol. 279, No. 26, 25.06.2004, p. 27584-27590.

Research output: Contribution to journalArticle

Moore, JMR, Galicia, SJ, McReynolds, AC, Nguyen, NH, Scanlan, TT & Guy, RK 2004, 'Quantitative proteomics of the thyroid hormone receptor-coregulator interactions', Journal of Biological Chemistry, vol. 279, no. 26, pp. 27584-27590. https://doi.org/10.1074/jbc.M403453200
Moore, Jamie M R ; Galicia, Sarah J. ; McReynolds, Andrea C. ; Nguyen, Ngoc Ha ; Scanlan, Thomas (Tom) ; Guy, R. Kiplin. / Quantitative proteomics of the thyroid hormone receptor-coregulator interactions. In: Journal of Biological Chemistry. 2004 ; Vol. 279, No. 26. pp. 27584-27590.
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