TY - JOUR
T1 - Quantitative neuroproteomics of the synapse.
AU - Ramos-Ortolaza, Dinah Lee
AU - Bushlin, Ittai
AU - Abul-Husn, Noura
AU - Annangudi, Suresh P.
AU - Sweedler, Jonathan
AU - Devi, Lakshmi A.
PY - 2010
Y1 - 2010
N2 - An emerging way to study neuropsychiatric or neurodegenerative diseases is by performing proteomic analyses of brain tissues. Here, we describe methods used to isolate and identify the proteins associated with a sample of interest, such as the synapse, as well as to compare the levels of proteins in the sample under different conditions. These techniques, involving subcellular fractionation and modern quantitative proteomics using isotopic labels, can be used to understand the organization of neuronal compartments and the regulation of synaptic function under various conditions.
AB - An emerging way to study neuropsychiatric or neurodegenerative diseases is by performing proteomic analyses of brain tissues. Here, we describe methods used to isolate and identify the proteins associated with a sample of interest, such as the synapse, as well as to compare the levels of proteins in the sample under different conditions. These techniques, involving subcellular fractionation and modern quantitative proteomics using isotopic labels, can be used to understand the organization of neuronal compartments and the regulation of synaptic function under various conditions.
UR - http://www.scopus.com/inward/record.url?scp=77449123424&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=77449123424&partnerID=8YFLogxK
U2 - 10.1007/978-1-60761-535-4_18
DO - 10.1007/978-1-60761-535-4_18
M3 - Article
C2 - 20013213
AN - SCOPUS:77449123424
SN - 1064-3745
VL - 615
SP - 227
EP - 246
JO - Methods in molecular biology (Clifton, N.J.)
JF - Methods in molecular biology (Clifton, N.J.)
ER -