Abstract
Purpose. To determine whether differences in lysozyme deposition and/or activity exist on worn etafilcon and balafilcon contact lenses following care with a polyquaternium-based system (PQ) or a polyhexanide-based system (PHMB). Methods. Following acid-based deposit extraction, lysozyme concentration was determined via Western blotting and lysozyme activity was determined by a micrococcyl assay. Results. Lysozyme deposition on etafilcon lenses was greater following disinfection with the PHMB-based system (1551 ± 371 μg/lens vs 935 ± 271 μg/lens; p < 0.001). Deposition on balafilcon lenses was not influenced by the care regimen (10 ± 3.5 μg/lens vs 10 ± 5 μg/lens; p = 0.89). For both materials, the percentage of denatured lysozyme was greater when they were exposed to the PHMB-based system (28 vs 21%; p = 0.05 (etafilcon) and 57 vs 40%; p = 0.04 (balafilcon)). Conclusions. The quantity and conformation of lysozyme deposited on hydrogel contact lens materials is significantly influenced by both lens material and care regimen.
Original language | English (US) |
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Pages (from-to) | 25-36 |
Number of pages | 12 |
Journal | Current Eye Research |
Volume | 28 |
Issue number | 1 |
DOIs | |
State | Published - Jan 2004 |
Externally published | Yes |
Keywords
- Hydrogel contact lens
- Lysozyme
- Protein denaturation
- Protein extraction
- Western blotting
ASJC Scopus subject areas
- Ophthalmology
- Sensory Systems
- Cellular and Molecular Neuroscience