Quantitative analysis of the proliferative activity induced in murine thymocytes by Concanavalin A

S. Gerhart, G. Mills, V. V. Monticone andPaetkau

    Research output: Contribution to journalArticle

    9 Scopus citations

    Abstract

    A quantitative analysis of the proliferative response induced in murine thymocytes by concanavalin A (Con A) is described. Exogenous 3H thymidine labels 35 to 40% of the newly incorporated TMP residues under optimal conditions. The density label 5 bromo 2' deoxuridine (BrUdR) does not affect DNA metabolism in this system. With this nucleoside, it is shown that newly synthesized DNA is the result of semi conservative replication, not repair. Double labeling of DNA provides a monitor for cells traversing the cell cycle (S phase to subsequent S phase). The average cycle time is 12.5 hr, and the shortest cell cycle time is 10 hr. The growing fraction of active cells is about two thirds. The data show that different subpopulations of thymocytes begin proliferating after various times in culture. Once effectively stimulated by Con A, some of the cells can traverse the cell cycle at least twice more after the mitogen is removed.

    Original languageEnglish (US)
    Pages (from-to)1314-1319
    Number of pages6
    JournalJournal of Immunology
    Volume117
    Issue number4
    StatePublished - Dec 1 1976

    ASJC Scopus subject areas

    • Immunology and Allergy
    • Immunology

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