TY - JOUR
T1 - Quantitative analysis of the proliferative activity induced in murine thymocytes by Concanavalin A
AU - Gerhart, S.
AU - Mills, G.
AU - Monticone andPaetkau, V. V.
PY - 1976
Y1 - 1976
N2 - A quantitative analysis of the proliferative response induced in murine thymocytes by concanavalin A (Con A) is described. Exogenous 3H thymidine labels 35 to 40% of the newly incorporated TMP residues under optimal conditions. The density label 5 bromo 2' deoxuridine (BrUdR) does not affect DNA metabolism in this system. With this nucleoside, it is shown that newly synthesized DNA is the result of semi conservative replication, not repair. Double labeling of DNA provides a monitor for cells traversing the cell cycle (S phase to subsequent S phase). The average cycle time is 12.5 hr, and the shortest cell cycle time is 10 hr. The growing fraction of active cells is about two thirds. The data show that different subpopulations of thymocytes begin proliferating after various times in culture. Once effectively stimulated by Con A, some of the cells can traverse the cell cycle at least twice more after the mitogen is removed.
AB - A quantitative analysis of the proliferative response induced in murine thymocytes by concanavalin A (Con A) is described. Exogenous 3H thymidine labels 35 to 40% of the newly incorporated TMP residues under optimal conditions. The density label 5 bromo 2' deoxuridine (BrUdR) does not affect DNA metabolism in this system. With this nucleoside, it is shown that newly synthesized DNA is the result of semi conservative replication, not repair. Double labeling of DNA provides a monitor for cells traversing the cell cycle (S phase to subsequent S phase). The average cycle time is 12.5 hr, and the shortest cell cycle time is 10 hr. The growing fraction of active cells is about two thirds. The data show that different subpopulations of thymocytes begin proliferating after various times in culture. Once effectively stimulated by Con A, some of the cells can traverse the cell cycle at least twice more after the mitogen is removed.
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M3 - Article
C2 - 1086322
AN - SCOPUS:0017168728
SN - 0022-1767
VL - 117
SP - 1314
EP - 1319
JO - Journal of Immunology
JF - Journal of Immunology
IS - 4
ER -