Quantification of vestibular-induced eye movements in zebrafish larvae

Weike Mo, Fangyi Chen, Alex Nechiporuk, Teresa Nicolson

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

Background: Vestibular reflexes coordinate movements or sensory input with changes in body or head position. Vestibular-evoked responses that involve the extraocular muscles include the vestibulo-ocular reflex (VOR), a compensatory eye movement to stabilize retinal images. Although an angular VOR attributable to semicircular canal stimulation was reported to be absent in free-swimming zebrafish larvae, recent studies reveal that vestibular-induced eye movements can be evoked in zebrafish larvae by both static tilts and dynamic rotations that tilt the head with respect to gravity. Results: We have determined herein the basis of sensitivity of the larval eye movements with respect to vestibular stimulus, developmental stage, and sensory receptors of the inner ear. For our experiments, video recordings of larvae rotated sinusoidally at 0.25 Hz were analyzed to quantitate eye movements under infrared illumination. We observed a robust response that appeared as early as 72 hours post fertilization (hpf), which increased in amplitude over time. Unlike rotation about an earth horizontal axis, rotation about an earth vertical axis at 0.25 Hz did not evoke eye movements. Moreover, vestibular-induced responses were absent in mutant cdh23 larvae and larvae lacking anterior otoliths. Conclusions: Our results provide evidence for a functional vestibulo-oculomotor circuit in 72 hpf zebrafish larvae that relies upon sensory input from anterior/utricular otolith organs.

Original languageEnglish (US)
Pages (from-to)110
Number of pages1
JournalBMC Neuroscience
DOIs
StateAccepted/In press - Sep 3 2010
Externally publishedYes

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Zebrafish
Eye Movements
Larva
Vestibulo-Ocular Reflex
Otolithic Membrane
Fertilization
Oculomotor Muscles
Head
Semicircular Canals
Video Recording
Gravitation
Sensory Receptor Cells
Inner Ear
Lighting
Reflex

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience
  • Neuroscience(all)

Cite this

Quantification of vestibular-induced eye movements in zebrafish larvae. / Mo, Weike; Chen, Fangyi; Nechiporuk, Alex; Nicolson, Teresa.

In: BMC Neuroscience, 03.09.2010, p. 110.

Research output: Contribution to journalArticle

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