Quantification of thyromimetic sobetirome concentration in biological tissue samples

Jordan Devereaux, Skylar J. Ferrara, Thomas (Tom) Scanlan

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Thyroid hormone is a principal regulator of essential processes in vertebrate physiology and homeostasis. Synthetic derivatives of thyroid hormone, known as thyromimetics, display desirable therapeutic properties. Thoroughly understanding how thyromimetics distribute throughout the body is crucial for their development and this requires appropriate bioanalytical techniques to quantify drug levels in different tissues. Here, we describe a detailed protocol for the quantification of the thyromimetic sobetirome using liquid chromatography tandem-mass spectrometry (LC-MS/MS).

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages193-206
Number of pages14
DOIs
StatePublished - Jan 1 2018

Publication series

NameMethods in Molecular Biology
Volume1801
ISSN (Print)1064-3745

Fingerprint

Thyroid Hormones
Tandem Mass Spectrometry
Liquid Chromatography
Vertebrates
Homeostasis
Pharmaceutical Preparations
GC 1 compound
Therapeutics

Keywords

  • Drug quantification
  • LC-MS/MS
  • Pharmacokinetics
  • PK-ADME
  • Sobetirome
  • Thyroid hormone
  • Thyromimetic

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Devereaux, J., Ferrara, S. J., & Scanlan, T. T. (2018). Quantification of thyromimetic sobetirome concentration in biological tissue samples. In Methods in Molecular Biology (pp. 193-206). (Methods in Molecular Biology; Vol. 1801). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-7902-8_16

Quantification of thyromimetic sobetirome concentration in biological tissue samples. / Devereaux, Jordan; Ferrara, Skylar J.; Scanlan, Thomas (Tom).

Methods in Molecular Biology. Humana Press Inc., 2018. p. 193-206 (Methods in Molecular Biology; Vol. 1801).

Research output: Chapter in Book/Report/Conference proceedingChapter

Devereaux, J, Ferrara, SJ & Scanlan, TT 2018, Quantification of thyromimetic sobetirome concentration in biological tissue samples. in Methods in Molecular Biology. Methods in Molecular Biology, vol. 1801, Humana Press Inc., pp. 193-206. https://doi.org/10.1007/978-1-4939-7902-8_16
Devereaux J, Ferrara SJ, Scanlan TT. Quantification of thyromimetic sobetirome concentration in biological tissue samples. In Methods in Molecular Biology. Humana Press Inc. 2018. p. 193-206. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-7902-8_16
Devereaux, Jordan ; Ferrara, Skylar J. ; Scanlan, Thomas (Tom). / Quantification of thyromimetic sobetirome concentration in biological tissue samples. Methods in Molecular Biology. Humana Press Inc., 2018. pp. 193-206 (Methods in Molecular Biology).
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