Quantification of thyromimetic sobetirome concentration in biological tissue samples

Jordan Devereaux, Skylar J. Ferrara, Thomas (Tom) Scanlan

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Thyroid hormone is a principal regulator of essential processes in vertebrate physiology and homeostasis. Synthetic derivatives of thyroid hormone, known as thyromimetics, display desirable therapeutic properties. Thoroughly understanding how thyromimetics distribute throughout the body is crucial for their development and this requires appropriate bioanalytical techniques to quantify drug levels in different tissues. Here, we describe a detailed protocol for the quantification of the thyromimetic sobetirome using liquid chromatography tandem-mass spectrometry (LC-MS/MS).

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages193-206
Number of pages14
DOIs
Publication statusPublished - Jan 1 2018

Publication series

NameMethods in Molecular Biology
Volume1801
ISSN (Print)1064-3745

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Keywords

  • Drug quantification
  • LC-MS/MS
  • Pharmacokinetics
  • PK-ADME
  • Sobetirome
  • Thyroid hormone
  • Thyromimetic

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Devereaux, J., Ferrara, S. J., & Scanlan, T. T. (2018). Quantification of thyromimetic sobetirome concentration in biological tissue samples. In Methods in Molecular Biology (pp. 193-206). (Methods in Molecular Biology; Vol. 1801). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-7902-8_16