Quantification of DNA binding to cell-surfaces by flow cytometry

Sharon L. McCoy, Frances A. Hausman, Mark E. Deffebach, Antony Bakke, Louise S. Merkens, Robert (Rob) Bennett, Steven Hefeneider

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

DNA binding to cell-surfaces has been documented in several studies. The interaction of DNA with cells has been shown to have therapeutic potential as a non-viral form of gene delivery and DNA vaccination. Recently, bacterial DNA binding and internalization has been demonstrated in some cells to trigger secretion of cytokines and cell activation. Previous studies to quantify DNA binding to cells have used radiolabeled DNA. Here we report a non-radioactive assay for quantification of cell-surface DNA binding based on the isoparametric analysis of flow cytometric data as described by Chatelier et al., Embo J., 5 (1986) 1181. This assay has the advantage over previously used procedures in not employing radioactive material and being able to discriminate viable from non-viable cells that bind DNA. With the importance of understanding the interaction of DNA with cells, this assay may have application for the identification and characterization of reagents designed to either enhance or inhibit DNA binding to cells. Copyright (C) 2000 Elsevier Science B.V.

Original languageEnglish (US)
Pages (from-to)141-146
Number of pages6
JournalJournal of Immunological Methods
Volume241
Issue number1-2
DOIs
StatePublished - Jul 31 2000

Fingerprint

Flow Cytometry
DNA
Bacterial DNA
Vaccination
Cytokines
Genes

Keywords

  • DNA binding
  • Flow cytometry
  • Isoparametric analysis

ASJC Scopus subject areas

  • Biotechnology
  • Immunology

Cite this

McCoy, S. L., Hausman, F. A., Deffebach, M. E., Bakke, A., Merkens, L. S., Bennett, R. R., & Hefeneider, S. (2000). Quantification of DNA binding to cell-surfaces by flow cytometry. Journal of Immunological Methods, 241(1-2), 141-146. https://doi.org/10.1016/S0022-1759(00)00205-2

Quantification of DNA binding to cell-surfaces by flow cytometry. / McCoy, Sharon L.; Hausman, Frances A.; Deffebach, Mark E.; Bakke, Antony; Merkens, Louise S.; Bennett, Robert (Rob); Hefeneider, Steven.

In: Journal of Immunological Methods, Vol. 241, No. 1-2, 31.07.2000, p. 141-146.

Research output: Contribution to journalArticle

McCoy, SL, Hausman, FA, Deffebach, ME, Bakke, A, Merkens, LS, Bennett, RR & Hefeneider, S 2000, 'Quantification of DNA binding to cell-surfaces by flow cytometry', Journal of Immunological Methods, vol. 241, no. 1-2, pp. 141-146. https://doi.org/10.1016/S0022-1759(00)00205-2
McCoy SL, Hausman FA, Deffebach ME, Bakke A, Merkens LS, Bennett RR et al. Quantification of DNA binding to cell-surfaces by flow cytometry. Journal of Immunological Methods. 2000 Jul 31;241(1-2):141-146. https://doi.org/10.1016/S0022-1759(00)00205-2
McCoy, Sharon L. ; Hausman, Frances A. ; Deffebach, Mark E. ; Bakke, Antony ; Merkens, Louise S. ; Bennett, Robert (Rob) ; Hefeneider, Steven. / Quantification of DNA binding to cell-surfaces by flow cytometry. In: Journal of Immunological Methods. 2000 ; Vol. 241, No. 1-2. pp. 141-146.
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