Quantification of cytomegalovirus DNA in peripheral blood leukocytes by a branched-DNA signal amplification assay

David N. Chernoff, Richard C. Miner, Bradley S. Hoo, Lu Ping Shen, Reed J. Kelso, Dragana Jekic-McMullen, Jacob P. Lalezari, Sunwen Chou, W. Lawrence Drew, Janice A. Kolberg

Research output: Contribution to journalArticle

27 Scopus citations

Abstract

Quantification of cytomegalovirus (CMV) DNA in blood may aid in the identification of patients at highest risk for developing CMV disease, the evaluation of new therapeutics, and the prompt recognition of drug-resistant CMV strains. A branched-DNA (bDNA) assay was developed for the reliable quantification of CMV DNA in peripheral blood leukocytes. The bDNA assay allowed for the highly specific and reproducible quantification of CMV DNA in clinical specimens. Furthermore, the bDNA assay was at least as sensitive as culture techniques and displayed a nearly 3 log10 dynamic range in quantification. Changes in CMV DNA levels measured by the bDNA assay in a human immunodeficiency virus-positive patient undergoing therapy were consistent with CMV culture, antigen, and genotype results and correlated with disease progression and resistance markers. The bDNA assay for the quantification of CMV DNA may provide a useful tool that can be used to aid physicians in monitoring disease progression, evaluating therapeutic regimens, and recognizing viral resistance and drug failure.

Original languageEnglish (US)
Pages (from-to)2740-2744
Number of pages5
JournalJournal of Clinical Microbiology
Volume35
Issue number11
DOIs
StatePublished - Nov 1997

ASJC Scopus subject areas

  • Microbiology (medical)

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    Chernoff, D. N., Miner, R. C., Hoo, B. S., Shen, L. P., Kelso, R. J., Jekic-McMullen, D., Lalezari, J. P., Chou, S., Drew, W. L., & Kolberg, J. A. (1997). Quantification of cytomegalovirus DNA in peripheral blood leukocytes by a branched-DNA signal amplification assay. Journal of Clinical Microbiology, 35(11), 2740-2744. https://doi.org/10.1128/jcm.35.11.2740-2744.1997