Quality Control Protocol for Confocal Systems

Anda Cornea

Research output: Chapter in Book/Report/Conference proceedingChapter


Quantitative analysis of confocal imaging experiments require more stringent quality control of instrument function than qualitative imaging. Unfortunately, there are no standard procedures for quality control that are uniformly implemented, and, in multi user facilities experimenters rarely have access to the QC information. This paper proposes an easy and very efficient protocol that could be performed at the beginning of each day, experiment or even slide. It takes only a few minutes to assess laser stability, stage stability, channel registration in 3 dimensions and flatness of field. The information may be used either to calibrate data or, in more severe cases to request servicing the instrument.

Original languageEnglish (US)
Title of host publicationMethods in Cell Biology
PublisherAcademic Press Inc.
Number of pages8
StatePublished - 2013
Externally publishedYes

Publication series

NameMethods in Cell Biology
ISSN (Print)0091-679X


  • Confocal field uniformity
  • Confocal microscopy
  • Laser scanning
  • Laser stability
  • Quality control
  • Stage stability

ASJC Scopus subject areas

  • Cell Biology


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