Quality Control Protocol for Confocal Systems

Anda Cornea

    Research output: Chapter in Book/Report/Conference proceedingChapter

    Abstract

    Quantitative analysis of confocal imaging experiments require more stringent quality control of instrument function than qualitative imaging. Unfortunately, there are no standard procedures for quality control that are uniformly implemented, and, in multi user facilities experimenters rarely have access to the QC information. This paper proposes an easy and very efficient protocol that could be performed at the beginning of each day, experiment or even slide. It takes only a few minutes to assess laser stability, stage stability, channel registration in 3 dimensions and flatness of field. The information may be used either to calibrate data or, in more severe cases to request servicing the instrument.

    Original languageEnglish (US)
    Title of host publicationMethods in Cell Biology
    PublisherAcademic Press Inc.
    Pages51-58
    Number of pages8
    DOIs
    StatePublished - 2013

    Publication series

    NameMethods in Cell Biology
    Volume113
    ISSN (Print)0091-679X

    Keywords

    • Confocal field uniformity
    • Confocal microscopy
    • Laser scanning
    • Laser stability
    • Quality control
    • Stage stability

    ASJC Scopus subject areas

    • Cell Biology

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  • Cite this

    Cornea, A. (2013). Quality Control Protocol for Confocal Systems. In Methods in Cell Biology (pp. 51-58). (Methods in Cell Biology; Vol. 113). Academic Press Inc.. https://doi.org/10.1016/B978-0-12-407239-8.00003-3