Putative pain modulating neurons in the rostral ventral medulla: Reflex-related activity predicts effects of morphine

Nicholas M. Barbaro, Mary Heinricher, Howard L. Fields

Research output: Contribution to journalArticle

128 Citations (Scopus)

Abstract

Three physiologically-defined classes of neurons are found in the rostral ventromedial medulla (RVM), a region which contributes to the antinociceptive action of opiates. The off-cell exhibits an abrupt pause just prior to the occurrence of the tail flick reflex (TF). The on-cell shows a burst of activity beginning just prior to the occurrence of the TF. Neutral cell firing does not change in relation to the TF. Systematic administration of morphine has been shown to produce a consistent increase in the activity of off-cells. In the present studies, the effects of systemically-administered morphine on spontaneous and TF-related activity of on-cells and neutral cells were examined in lightly-anesthetized rats. Measures of spontaneous activity were obtained before and after morphine (1.25-2.5 mg/kg, i.v.). On cell exhibited an irregular cyclic rate of spontaneous discharge similar to that previously reported for off-cells. In contrast, neutral cells had a nearly constant level of spontaneous activity. After administration of morphine, spontaneous activity ceased for 8 of 8 on-cells, and heat-related activity was eliminated. Administration of naloxone resulted in a return of the periodic firing pattern and the burst associated with the TF. Seven of 8 neutral cells showed no change in firing rate and one showed a decreased rate after morphine administration. These results show that the effect of systemic opiates on an RVM neuron can be predicted based on whether a cell increases or decreases its firing just prior to the occurrence of a nocifensive reflex. They add to the existing body of evidence implicating both on-and off-cells in modulation of nociceptive transmission and nocifensive reflexes at spinal levels and indicate that these neurons contribute to opiate-induced analgesia.

Original languageEnglish (US)
Pages (from-to)203-210
Number of pages8
JournalBrain Research
Volume366
Issue number1-2
DOIs
StatePublished - Feb 26 1986
Externally publishedYes

Fingerprint

Morphine
Reflex
Neurons
Pain
Opiate Alkaloids
Tail
Naloxone
Analgesia
Hot Temperature

Keywords

  • analgesia
  • morphine
  • nucleus raphe magnus
  • rat
  • tail flick reflex

ASJC Scopus subject areas

  • Developmental Biology
  • Molecular Biology
  • Clinical Neurology
  • Neuroscience(all)

Cite this

Putative pain modulating neurons in the rostral ventral medulla : Reflex-related activity predicts effects of morphine. / Barbaro, Nicholas M.; Heinricher, Mary; Fields, Howard L.

In: Brain Research, Vol. 366, No. 1-2, 26.02.1986, p. 203-210.

Research output: Contribution to journalArticle

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