Purified bovine NF-kappa B recognizes regulatory sequences in multiple genes expressed during activation of T- and B-lymphocytes.

M. J. Lenardo, Anna Kuang, A. Gifford, D. Baltimore

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

To characterize the NF-kappa B binding factor in molecular terms and to facilitate the cloning of its gene, we have purified this protein from bovine spleen tissue. We have found it is a 42,000-dalton protein that exists in solution as a dimer. We were able to use the purified protein to show that the same polypeptide is able to recognize sites important for activation of genes in either B- or T-lymphocytes. Moreover, we were able to define a consensus sequence which allows ascertainment of a wider variety of sequences that are capable of interacting with this protein. The implication of the same protein in gene regulation in two different lineages of lymphoid cells reveals an unexpected unity in the mechanism of gene expression during B- and T-lymphocyte activation. This also suggests that other regulatory events must participate with NF-kappa B activation in determining B- or T-cell-specific expression.

Original languageEnglish (US)
Pages (from-to)411-415
Number of pages5
JournalHamatologie und Bluttransfusion
Volume32
StatePublished - 1989
Externally publishedYes

Fingerprint

NF-kappa B
B-Lymphocytes
Genes
Proteins
T-Lymphocytes
Consensus Sequence
Lymphocyte Activation
Transcriptional Activation
Organism Cloning
Spleen
Lymphocytes
Gene Expression
Peptides

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Purified bovine NF-kappa B recognizes regulatory sequences in multiple genes expressed during activation of T- and B-lymphocytes. / Lenardo, M. J.; Kuang, Anna; Gifford, A.; Baltimore, D.

In: Hamatologie und Bluttransfusion, Vol. 32, 1989, p. 411-415.

Research output: Contribution to journalArticle

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