Purification of a mutant ribonucleotide reductase from cultured mouse T-lymphoma cells

Lorraine Gudas; Staffan Eriksson; Buddy Ullman; David Martin

doi:10.1016/0065-2571(81)90012-1

Purification of a mutant ribonucleotide reductase from cultured mouse T-lymphoma cells

Lorraine Gudas, Staffan Eriksson, Buddy Ullman, David Martin

Research output: Contribution to journal › Article › peer-review

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Abstract

Ribonucleotide reductase has been partially purified from a mutant line of S49 mouse T-lymphoma cells which is less sensitive to normal feedback control by deoxyATP. The mutation for deoxyATP feedback resistance resides in the M1 subunit in this mutant. The mutant appears to contain two alleles for ribonucleotide reductase, a mutant allele and a wild type allele, which can be separated by affinity chromatography on deoxyATP-Sepharose.

Original language	English (US)
Pages (from-to)	129-137
Number of pages	9
Journal	Advances in Enzyme Regulation
Volume	19
Issue number	C
DOIs	https://doi.org/10.1016/0065-2571(81)90012-1
State	Published - 1981
Externally published	Yes

ASJC Scopus subject areas

Molecular Medicine
Molecular Biology
Genetics
Cancer Research

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title = "Purification of a mutant ribonucleotide reductase from cultured mouse T-lymphoma cells",

abstract = "Ribonucleotide reductase has been partially purified from a mutant line of S49 mouse T-lymphoma cells which is less sensitive to normal feedback control by deoxyATP. The mutation for deoxyATP feedback resistance resides in the M1 subunit in this mutant. The mutant appears to contain two alleles for ribonucleotide reductase, a mutant allele and a wild type allele, which can be separated by affinity chromatography on deoxyATP-Sepharose.",

author = "Lorraine Gudas and Staffan Eriksson and Buddy Ullman and David Martin",

year = "1981",

doi = "10.1016/0065-2571(81)90012-1",

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T1 - Purification of a mutant ribonucleotide reductase from cultured mouse T-lymphoma cells

AU - Gudas, Lorraine

AU - Eriksson, Staffan

AU - Ullman, Buddy

AU - Martin, David

PY - 1981

Y1 - 1981

N2 - Ribonucleotide reductase has been partially purified from a mutant line of S49 mouse T-lymphoma cells which is less sensitive to normal feedback control by deoxyATP. The mutation for deoxyATP feedback resistance resides in the M1 subunit in this mutant. The mutant appears to contain two alleles for ribonucleotide reductase, a mutant allele and a wild type allele, which can be separated by affinity chromatography on deoxyATP-Sepharose.

AB - Ribonucleotide reductase has been partially purified from a mutant line of S49 mouse T-lymphoma cells which is less sensitive to normal feedback control by deoxyATP. The mutation for deoxyATP feedback resistance resides in the M1 subunit in this mutant. The mutant appears to contain two alleles for ribonucleotide reductase, a mutant allele and a wild type allele, which can be separated by affinity chromatography on deoxyATP-Sepharose.

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JO - Advances in Enzyme Regulation

JF - Advances in Enzyme Regulation

IS - C

ER -

Purification of a mutant ribonucleotide reductase from cultured mouse T-lymphoma cells

Abstract

ASJC Scopus subject areas

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