Purification of a mutant ribonucleotide reductase from cultured mouse T-lymphoma cells

Lorraine Gudas, Staffan Eriksson, Buddy Ullman, David Martin

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

Ribonucleotide reductase has been partially purified from a mutant line of S49 mouse T-lymphoma cells which is less sensitive to normal feedback control by deoxyATP. The mutation for deoxyATP feedback resistance resides in the M1 subunit in this mutant. The mutant appears to contain two alleles for ribonucleotide reductase, a mutant allele and a wild type allele, which can be separated by affinity chromatography on deoxyATP-Sepharose.

Original languageEnglish (US)
Pages (from-to)129-137
Number of pages9
JournalAdvances in Enzyme Regulation
Volume19
Issue numberC
DOIs
StatePublished - 1981

ASJC Scopus subject areas

  • Molecular Medicine
  • Molecular Biology
  • Genetics
  • Cancer Research

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