Purification and culture of oligodendrocyte lineage cells

Jason C. Dugas, Ben Emery

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Oligodendrocytes are the cells of the vertebrate central nervous system responsible for forming myelin sheaths, which are essential for the rapid propagation of action potential. The formation of oligodendrocytes and myelin sheaths is tightly regulated, both temporally and spatially, by a number of extracellular and intracellular factors. For example, notch ligands, thyroid hormones, and mitogens such as platelet-derived growth factor (PDGF) and fibroblast growth factor (FGF) can all interact with oligodendrocyte precursor cell-expressed receptors to impact proliferation, differentiation, and myelin gene expression. To facilitate oligodendrocyte biology research, we have developed a technique using immunopanning to isolate different stages of the oligodendrocyte lineage, oligodendrocyte precursor cells and/or postmitotic oligodendrocytes, from postnatal rat or mouse brains. These cells can be cultured in defined, serum-free media in conditions that either promote differentiation into mature oligodendrocytes or continued proliferation as immature oligodendrocyte precursors. These cells represent an ideal system in which to study the regulation of oligodendrocyte proliferation, migration, differentiation, myelin gene expression, or other fundamental aspects of oligodendrocyte biology.

Original languageEnglish (US)
Pages (from-to)810-814
Number of pages5
JournalCold Spring Harbor Protocols
Volume2013
Issue number9
DOIs
StatePublished - 2013
Externally publishedYes

Fingerprint

Oligodendroglia
Gene expression
Purification
Fibroblast Growth Factors
Platelet-Derived Growth Factor
Serum-Free Culture Media
Neurology
Thyroid Hormones
Mitogens
Rats
Brain
Myelin Sheath
Ligands
Gene Expression
Action Potentials
Vertebrates
Central Nervous System

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Purification and culture of oligodendrocyte lineage cells. / Dugas, Jason C.; Emery, Ben.

In: Cold Spring Harbor Protocols, Vol. 2013, No. 9, 2013, p. 810-814.

Research output: Contribution to journalArticle

@article{4ac934368f474b548517da19ffbfb55b,
title = "Purification and culture of oligodendrocyte lineage cells",
abstract = "Oligodendrocytes are the cells of the vertebrate central nervous system responsible for forming myelin sheaths, which are essential for the rapid propagation of action potential. The formation of oligodendrocytes and myelin sheaths is tightly regulated, both temporally and spatially, by a number of extracellular and intracellular factors. For example, notch ligands, thyroid hormones, and mitogens such as platelet-derived growth factor (PDGF) and fibroblast growth factor (FGF) can all interact with oligodendrocyte precursor cell-expressed receptors to impact proliferation, differentiation, and myelin gene expression. To facilitate oligodendrocyte biology research, we have developed a technique using immunopanning to isolate different stages of the oligodendrocyte lineage, oligodendrocyte precursor cells and/or postmitotic oligodendrocytes, from postnatal rat or mouse brains. These cells can be cultured in defined, serum-free media in conditions that either promote differentiation into mature oligodendrocytes or continued proliferation as immature oligodendrocyte precursors. These cells represent an ideal system in which to study the regulation of oligodendrocyte proliferation, migration, differentiation, myelin gene expression, or other fundamental aspects of oligodendrocyte biology.",
author = "Dugas, {Jason C.} and Ben Emery",
year = "2013",
doi = "10.1101/pdb.top074898",
language = "English (US)",
volume = "2013",
pages = "810--814",
journal = "Cold Spring Harbor Protocols",
issn = "1559-6095",
publisher = "Cold Spring Harbor Laboratory Press",
number = "9",

}

TY - JOUR

T1 - Purification and culture of oligodendrocyte lineage cells

AU - Dugas, Jason C.

AU - Emery, Ben

PY - 2013

Y1 - 2013

N2 - Oligodendrocytes are the cells of the vertebrate central nervous system responsible for forming myelin sheaths, which are essential for the rapid propagation of action potential. The formation of oligodendrocytes and myelin sheaths is tightly regulated, both temporally and spatially, by a number of extracellular and intracellular factors. For example, notch ligands, thyroid hormones, and mitogens such as platelet-derived growth factor (PDGF) and fibroblast growth factor (FGF) can all interact with oligodendrocyte precursor cell-expressed receptors to impact proliferation, differentiation, and myelin gene expression. To facilitate oligodendrocyte biology research, we have developed a technique using immunopanning to isolate different stages of the oligodendrocyte lineage, oligodendrocyte precursor cells and/or postmitotic oligodendrocytes, from postnatal rat or mouse brains. These cells can be cultured in defined, serum-free media in conditions that either promote differentiation into mature oligodendrocytes or continued proliferation as immature oligodendrocyte precursors. These cells represent an ideal system in which to study the regulation of oligodendrocyte proliferation, migration, differentiation, myelin gene expression, or other fundamental aspects of oligodendrocyte biology.

AB - Oligodendrocytes are the cells of the vertebrate central nervous system responsible for forming myelin sheaths, which are essential for the rapid propagation of action potential. The formation of oligodendrocytes and myelin sheaths is tightly regulated, both temporally and spatially, by a number of extracellular and intracellular factors. For example, notch ligands, thyroid hormones, and mitogens such as platelet-derived growth factor (PDGF) and fibroblast growth factor (FGF) can all interact with oligodendrocyte precursor cell-expressed receptors to impact proliferation, differentiation, and myelin gene expression. To facilitate oligodendrocyte biology research, we have developed a technique using immunopanning to isolate different stages of the oligodendrocyte lineage, oligodendrocyte precursor cells and/or postmitotic oligodendrocytes, from postnatal rat or mouse brains. These cells can be cultured in defined, serum-free media in conditions that either promote differentiation into mature oligodendrocytes or continued proliferation as immature oligodendrocyte precursors. These cells represent an ideal system in which to study the regulation of oligodendrocyte proliferation, migration, differentiation, myelin gene expression, or other fundamental aspects of oligodendrocyte biology.

UR - http://www.scopus.com/inward/record.url?scp=84883469861&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84883469861&partnerID=8YFLogxK

U2 - 10.1101/pdb.top074898

DO - 10.1101/pdb.top074898

M3 - Article

C2 - 24003197

AN - SCOPUS:84883469861

VL - 2013

SP - 810

EP - 814

JO - Cold Spring Harbor Protocols

JF - Cold Spring Harbor Protocols

SN - 1559-6095

IS - 9

ER -