Purification and characterization of metallothionein and its activation of pyridoxal phosphokinase in trout (Salmo gairdneri) brain

R. Hao, R. F. Pfeiffer, M. Ebadi

Research output: Contribution to journalArticle

15 Scopus citations

Abstract

1. 1. Brain metallothionein was isolated and purified for the first time from rainbow trout. 2. 2. Brain metallothionein exhibited an elution volume (Ve/Vo) of 2.0, had a molecular weight of 6762 Daltons, and contained a zinc content of 9 μg/mg protein. 3. 3. Brain pyridoxal phosphokinase was isolated and assayed for the first time in rainbow trout. 4. 4. Zinc (0.20 μM) or zinc metallothionein (6-30 μM) stimulated the activity of brain pyridoxal kinase in a linear fashion. 5. 5. The results of these studies are interpreted to suggest that in trout brain zinc metallothionein may participate in metabolism of vitamin B6 and formation of pyridoxal phosphate, the active coenzyme.

Original languageEnglish (US)
Pages (from-to)293-298
Number of pages6
JournalComparative Biochemistry and Physiology -- Part B: Biochemistry and
Volume104
Issue number2
DOIs
StatePublished - Feb 1993

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Molecular Biology

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