Purification and characterization of metallothionein and its activation of pyridoxal phosphokinase in trout (Salmo gairdneri) brain

R. Hao; R. F. Pfeiffer; M. Ebadi

doi:10.1016/0305-0491(93)90371-B

Purification and characterization of metallothionein and its activation of pyridoxal phosphokinase in trout (Salmo gairdneri) brain

R. Hao, R. F. Pfeiffer, M. Ebadi

Neurology

Research output: Contribution to journal › Article › peer-review

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Abstract

1. 1. Brain metallothionein was isolated and purified for the first time from rainbow trout. 2. 2. Brain metallothionein exhibited an elution volume (V_e/V_o) of 2.0, had a molecular weight of 6762 Daltons, and contained a zinc content of 9 μg/mg protein. 3. 3. Brain pyridoxal phosphokinase was isolated and assayed for the first time in rainbow trout. 4. 4. Zinc (0.20 μM) or zinc metallothionein (6-30 μM) stimulated the activity of brain pyridoxal kinase in a linear fashion. 5. 5. The results of these studies are interpreted to suggest that in trout brain zinc metallothionein may participate in metabolism of vitamin B₆ and formation of pyridoxal phosphate, the active coenzyme.

Original language	English (US)
Pages (from-to)	293-298
Number of pages	6
Journal	Comparative Biochemistry and Physiology -- Part B: Biochemistry and
Volume	104
Issue number	2
DOIs	https://doi.org/10.1016/0305-0491(93)90371-B
State	Published - Feb 1993

ASJC Scopus subject areas

Biochemistry
Physiology
Molecular Biology

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AU - Pfeiffer, R. F.

AU - Ebadi, M.

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N2 - 1. 1. Brain metallothionein was isolated and purified for the first time from rainbow trout. 2. 2. Brain metallothionein exhibited an elution volume (Ve/Vo) of 2.0, had a molecular weight of 6762 Daltons, and contained a zinc content of 9 μg/mg protein. 3. 3. Brain pyridoxal phosphokinase was isolated and assayed for the first time in rainbow trout. 4. 4. Zinc (0.20 μM) or zinc metallothionein (6-30 μM) stimulated the activity of brain pyridoxal kinase in a linear fashion. 5. 5. The results of these studies are interpreted to suggest that in trout brain zinc metallothionein may participate in metabolism of vitamin B6 and formation of pyridoxal phosphate, the active coenzyme.

AB - 1. 1. Brain metallothionein was isolated and purified for the first time from rainbow trout. 2. 2. Brain metallothionein exhibited an elution volume (Ve/Vo) of 2.0, had a molecular weight of 6762 Daltons, and contained a zinc content of 9 μg/mg protein. 3. 3. Brain pyridoxal phosphokinase was isolated and assayed for the first time in rainbow trout. 4. 4. Zinc (0.20 μM) or zinc metallothionein (6-30 μM) stimulated the activity of brain pyridoxal kinase in a linear fashion. 5. 5. The results of these studies are interpreted to suggest that in trout brain zinc metallothionein may participate in metabolism of vitamin B6 and formation of pyridoxal phosphate, the active coenzyme.

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Purification and characterization of metallothionein and its activation of pyridoxal phosphokinase in trout (Salmo gairdneri) brain

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