Pupil segmentation adaptive optics for in vivo mouse retinal fluorescence imaging

Daniel J. Wahl, Christine Huang, Stefano Bonora, Yifan Jian, Marinko V.S. Arunic

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Adaptive Optics (AO) for scanning laser ophthalmoscopy enables high-resolution retinal imaging that can be used for preclinical research of diseases causing vision loss. Pupil Segmentation (PS) is an approach to wavefront-sensorless AO that acquires images within subregions across the imaging pupil to measure the wavefront slopes at the corresponding locations of the beam. We present PS-AO as an approach to correct ocular aberrations in 7 s, implemented to minimize respiratory motion from an anesthetized mouse. We demonstrated an improvement in resolution and an image intensity increase of 25% across all results using PS-AO for in vivo fluorescence retinal imaging in mice using a MEMS-based segmented deformable mirror.

Original languageEnglish (US)
Pages (from-to)1365-1368
Number of pages4
JournalOptics Letters
Volume42
Issue number7
DOIs
StatePublished - Apr 1 2017
Externally publishedYes

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pupils
adaptive optics
mice
fluorescence
segmented mirrors
deformable mirrors
microelectromechanical systems
aberration
slopes
scanning
high resolution
lasers

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics

Cite this

Pupil segmentation adaptive optics for in vivo mouse retinal fluorescence imaging. / Wahl, Daniel J.; Huang, Christine; Bonora, Stefano; Jian, Yifan; Arunic, Marinko V.S.

In: Optics Letters, Vol. 42, No. 7, 01.04.2017, p. 1365-1368.

Research output: Contribution to journalArticle

Wahl, Daniel J. ; Huang, Christine ; Bonora, Stefano ; Jian, Yifan ; Arunic, Marinko V.S. / Pupil segmentation adaptive optics for in vivo mouse retinal fluorescence imaging. In: Optics Letters. 2017 ; Vol. 42, No. 7. pp. 1365-1368.
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