Pseudomonas aeruginosa induces membrane blebs in epithelial cells, which are utilized as a niche for intracellular replication and motility

Annette A. Angus, Amanda Ackerman Lee, Danielle K. Augustin, Ellen Lee, David J. Evans, Suzanne M J Fleiszig

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

Pseudomonas aeruginosa is known to invade epithelial cells during infection and in vitro. However, little is known of bacterial or epithelial factors modulating P. aeruginosa intracellular survival or replication after invasion, except that it requires a complete lipopolysaccharide core. In this study, real-time video microscopy revealed that invasive P. aeruginosa isolates induced the formation of membrane blebs in multiple epithelial cell types and that these were then exploited for intracellular replication and rapid real-time motility. Further studies revealed that the type three secretion system (T3SS) of P. aeruginosa was required for blebbing. Mutants lacking either the entire T3SS or specific T3SS components were instead localized to intracellular perinuclear vacuoles. Most T3SS mutants that trafficked to perinuclear vacuoles gradually lost intracellular viability, and vacuoles containing those bacteria were labeled by the late endosomal marker lysosome-associated marker protein 3 (LAMP-3). Interestingly, mutants deficient only in the T3SS translocon structure survived and replicated within the vacuoles that did not label with LAMP-3. Taken together, these data suggest two novel roles of the P. aeruginosa T3SS in enabling bacterial intracellular survival: translocon-dependent formation of membrane blebs, which form a host cell niche for bacterial growth and motility, and effector-dependent bacterial survival and replication within intracellular perinuclear vacuoles.

Original languageEnglish (US)
Pages (from-to)1992-2001
Number of pages10
JournalInfection and Immunity
Volume76
Issue number5
DOIs
StatePublished - May 2008

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Blister
Vacuoles
Pseudomonas aeruginosa
Epithelial Cells
Membranes
Lysosomes
Video Microscopy
Lipopolysaccharides
Proteins
Bacteria
Growth
Infection

ASJC Scopus subject areas

  • Immunology

Cite this

Pseudomonas aeruginosa induces membrane blebs in epithelial cells, which are utilized as a niche for intracellular replication and motility. / Angus, Annette A.; Lee, Amanda Ackerman; Augustin, Danielle K.; Lee, Ellen; Evans, David J.; Fleiszig, Suzanne M J.

In: Infection and Immunity, Vol. 76, No. 5, 05.2008, p. 1992-2001.

Research output: Contribution to journalArticle

Angus, Annette A. ; Lee, Amanda Ackerman ; Augustin, Danielle K. ; Lee, Ellen ; Evans, David J. ; Fleiszig, Suzanne M J. / Pseudomonas aeruginosa induces membrane blebs in epithelial cells, which are utilized as a niche for intracellular replication and motility. In: Infection and Immunity. 2008 ; Vol. 76, No. 5. pp. 1992-2001.
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