PSD-95 involvement in maturation of excitatory synapses

A. E.D. El-Husseini, E. Schnell, D. M. Chetkovich, R. A. Nicoll, D. S. Bredt

Research output: Contribution to journalArticlepeer-review

876 Scopus citations

Abstract

PSD-95 is a neuronal PDZ protein that associates with receptors and cytoskeletal elements at synapses, but whose function is uncertain. We found that overexpression of PSD-95 in hippocampal neurons can drive maturation of glutamatergic synapses. PSD-95 expression enhanced postsynaptic clustering and activity of glutamate receptors. Postsynaptic expression of PSD-95 also enhanced maturation of the presynaptic terminal. These effects required synaptic clustering of PSD-95 but did not rely on its guanylate kinase domain. PSD-95 expression also increased the number and size of dendritic spines. These results demonstrate that PSD-95 can orchestrate synaptic development and are suggestive of roles for PSD-95 in synapse stabilization and plasticity.

Original languageEnglish (US)
Pages (from-to)1364-1368
Number of pages5
JournalScience
Volume290
Issue number5495
StatePublished - Nov 17 2000
Externally publishedYes

ASJC Scopus subject areas

  • General

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