PSD-95 involvement in maturation of excitatory synapses

A. E.D. El-Husseini, Eric Schnell, D. M. Chetkovich, R. A. Nicoll, D. S. Bredt

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Abstract

PSD-95 is a neuronal PDZ protein that associates with receptors and cytoskeletal elements at synapses, but whose function is uncertain. We found that overexpression of PSD-95 in hippocampal neurons can drive maturation of glutamatergic synapses. PSD-95 expression enhanced postsynaptic clustering and activity of glutamate receptors. Postsynaptic expression of PSD-95 also enhanced maturation of the presynaptic terminal. These effects required synaptic clustering of PSD-95 but did not rely on its guanylate kinase domain. PSD-95 expression also increased the number and size of dendritic spines. These results demonstrate that PSD-95 can orchestrate synaptic development and are suggestive of roles for PSD-95 in synapse stabilization and plasticity.

Original languageEnglish (US)
Pages (from-to)1364-1368
Number of pages5
JournalScience
Volume290
Issue number5495
StatePublished - Nov 17 2000
Externally publishedYes

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Synapses
Cluster Analysis
Guanylate Kinases
Dendritic Spines
Presynaptic Terminals
Glutamate Receptors
Neurons
Proteins

ASJC Scopus subject areas

  • General

Cite this

El-Husseini, A. E. D., Schnell, E., Chetkovich, D. M., Nicoll, R. A., & Bredt, D. S. (2000). PSD-95 involvement in maturation of excitatory synapses. Science, 290(5495), 1364-1368.

PSD-95 involvement in maturation of excitatory synapses. / El-Husseini, A. E.D.; Schnell, Eric; Chetkovich, D. M.; Nicoll, R. A.; Bredt, D. S.

In: Science, Vol. 290, No. 5495, 17.11.2000, p. 1364-1368.

Research output: Contribution to journalArticle

El-Husseini, AED, Schnell, E, Chetkovich, DM, Nicoll, RA & Bredt, DS 2000, 'PSD-95 involvement in maturation of excitatory synapses', Science, vol. 290, no. 5495, pp. 1364-1368.
El-Husseini AED, Schnell E, Chetkovich DM, Nicoll RA, Bredt DS. PSD-95 involvement in maturation of excitatory synapses. Science. 2000 Nov 17;290(5495):1364-1368.
El-Husseini, A. E.D. ; Schnell, Eric ; Chetkovich, D. M. ; Nicoll, R. A. ; Bredt, D. S. / PSD-95 involvement in maturation of excitatory synapses. In: Science. 2000 ; Vol. 290, No. 5495. pp. 1364-1368.
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