PSD-95 involvement in maturation of excitatory synapses

A. E.D. El-Husseini, E. Schnell, D. M. Chetkovich, R. A. Nicoll, D. S. Bredt

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834 Scopus citations


PSD-95 is a neuronal PDZ protein that associates with receptors and cytoskeletal elements at synapses, but whose function is uncertain. We found that overexpression of PSD-95 in hippocampal neurons can drive maturation of glutamatergic synapses. PSD-95 expression enhanced postsynaptic clustering and activity of glutamate receptors. Postsynaptic expression of PSD-95 also enhanced maturation of the presynaptic terminal. These effects required synaptic clustering of PSD-95 but did not rely on its guanylate kinase domain. PSD-95 expression also increased the number and size of dendritic spines. These results demonstrate that PSD-95 can orchestrate synaptic development and are suggestive of roles for PSD-95 in synapse stabilization and plasticity.

Original languageEnglish (US)
Pages (from-to)1364-1368
Number of pages5
Issue number5495
StatePublished - Nov 17 2000

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    El-Husseini, A. E. D., Schnell, E., Chetkovich, D. M., Nicoll, R. A., & Bredt, D. S. (2000). PSD-95 involvement in maturation of excitatory synapses. Science, 290(5495), 1364-1368.