Proteomes of Paired Human Cerebrospinal Fluid and Plasma: Relation to Blood-Brain Barrier Permeability in Older Adults

Loïc Dayon, Ornella Cominetti, Jérôme Wojcik, Antonio Núñez Galindo, Aikaterini Oikonomidi, Hugues Henry, Eugenia Migliavacca, Martin Kussmann, Gene Bowman, Julius Popp

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The systems-level relationship between the proteomes of cerebrospinal fluid (CSF) and plasma has not been comprehensively described so far. Recently developed shotgun proteomic workflows allow for deeper characterization of the proteomes from body fluids in much larger sample size. We deployed state-of-the-art mass spectrometry-based proteomics in paired CSF and plasma samples volunteered by 120 elders with and without cognitive impairment to comprehensively characterize and examine compartmental proteome differences and relationships between both body fluids. We further assessed the influence of blood-brain barrier (BBB) integrity and tested the hypothesis that BBB breakdown can be identified from CSF and plasma proteome alterations in nondemented elders. We quantified 790 proteins in CSF and 422 proteins in plasma, and 255 of the proteins were identified in both compartments. Pearson's statistics determined 28 proteins with associated levels between CSF and plasma. BBB integrity as defined with the CSF/serum albumin index influenced 76 CSF/plasma protein ratios. In least absolute shrinkage and selection operator models, CSF and plasma proteins improved identification of BBB impairment. In conclusion, we provide here a first comprehensive draft map of interacting human CSF and plasma proteomes, in view of their complex and dynamic compositions, and influence of the BBB.

Original languageEnglish (US)
Pages (from-to)1162-1174
Number of pages13
JournalJournal of Proteome Research
Volume18
Issue number3
DOIs
StatePublished - Mar 1 2019
Externally publishedYes

Fingerprint

Cerebrospinal fluid
Proteome
Blood-Brain Barrier
Cerebrospinal Fluid
Permeability
Cerebrospinal Fluid Proteins
Plasmas
Blood Proteins
Body Fluids
Proteomics
Body fluids
Proteins
Workflow
Firearms
Serum Albumin
Sample Size
Mass Spectrometry
Mass spectrometry
Identification (control systems)
Statistics

Keywords

  • BBB
  • Blood
  • Brain
  • Cerebrospinal fluid
  • Circulation
  • Clinical proteomics
  • CSF
  • Mass spectrometry
  • Nervous system
  • Serum/plasma

ASJC Scopus subject areas

  • Biochemistry
  • Chemistry(all)

Cite this

Proteomes of Paired Human Cerebrospinal Fluid and Plasma : Relation to Blood-Brain Barrier Permeability in Older Adults. / Dayon, Loïc; Cominetti, Ornella; Wojcik, Jérôme; Galindo, Antonio Núñez; Oikonomidi, Aikaterini; Henry, Hugues; Migliavacca, Eugenia; Kussmann, Martin; Bowman, Gene; Popp, Julius.

In: Journal of Proteome Research, Vol. 18, No. 3, 01.03.2019, p. 1162-1174.

Research output: Contribution to journalArticle

Dayon, L, Cominetti, O, Wojcik, J, Galindo, AN, Oikonomidi, A, Henry, H, Migliavacca, E, Kussmann, M, Bowman, G & Popp, J 2019, 'Proteomes of Paired Human Cerebrospinal Fluid and Plasma: Relation to Blood-Brain Barrier Permeability in Older Adults', Journal of Proteome Research, vol. 18, no. 3, pp. 1162-1174. https://doi.org/10.1021/acs.jproteome.8b00809
Dayon, Loïc ; Cominetti, Ornella ; Wojcik, Jérôme ; Galindo, Antonio Núñez ; Oikonomidi, Aikaterini ; Henry, Hugues ; Migliavacca, Eugenia ; Kussmann, Martin ; Bowman, Gene ; Popp, Julius. / Proteomes of Paired Human Cerebrospinal Fluid and Plasma : Relation to Blood-Brain Barrier Permeability in Older Adults. In: Journal of Proteome Research. 2019 ; Vol. 18, No. 3. pp. 1162-1174.
@article{2ab87a8766834bfa9ebc8af78b458de7,
title = "Proteomes of Paired Human Cerebrospinal Fluid and Plasma: Relation to Blood-Brain Barrier Permeability in Older Adults",
abstract = "The systems-level relationship between the proteomes of cerebrospinal fluid (CSF) and plasma has not been comprehensively described so far. Recently developed shotgun proteomic workflows allow for deeper characterization of the proteomes from body fluids in much larger sample size. We deployed state-of-the-art mass spectrometry-based proteomics in paired CSF and plasma samples volunteered by 120 elders with and without cognitive impairment to comprehensively characterize and examine compartmental proteome differences and relationships between both body fluids. We further assessed the influence of blood-brain barrier (BBB) integrity and tested the hypothesis that BBB breakdown can be identified from CSF and plasma proteome alterations in nondemented elders. We quantified 790 proteins in CSF and 422 proteins in plasma, and 255 of the proteins were identified in both compartments. Pearson's statistics determined 28 proteins with associated levels between CSF and plasma. BBB integrity as defined with the CSF/serum albumin index influenced 76 CSF/plasma protein ratios. In least absolute shrinkage and selection operator models, CSF and plasma proteins improved identification of BBB impairment. In conclusion, we provide here a first comprehensive draft map of interacting human CSF and plasma proteomes, in view of their complex and dynamic compositions, and influence of the BBB.",
keywords = "BBB, Blood, Brain, Cerebrospinal fluid, Circulation, Clinical proteomics, CSF, Mass spectrometry, Nervous system, Serum/plasma",
author = "Lo{\"i}c Dayon and Ornella Cominetti and J{\'e}r{\^o}me Wojcik and Galindo, {Antonio N{\'u}{\~n}ez} and Aikaterini Oikonomidi and Hugues Henry and Eugenia Migliavacca and Martin Kussmann and Gene Bowman and Julius Popp",
year = "2019",
month = "3",
day = "1",
doi = "10.1021/acs.jproteome.8b00809",
language = "English (US)",
volume = "18",
pages = "1162--1174",
journal = "Journal of Proteome Research",
issn = "1535-3893",
publisher = "American Chemical Society",
number = "3",

}

TY - JOUR

T1 - Proteomes of Paired Human Cerebrospinal Fluid and Plasma

T2 - Relation to Blood-Brain Barrier Permeability in Older Adults

AU - Dayon, Loïc

AU - Cominetti, Ornella

AU - Wojcik, Jérôme

AU - Galindo, Antonio Núñez

AU - Oikonomidi, Aikaterini

AU - Henry, Hugues

AU - Migliavacca, Eugenia

AU - Kussmann, Martin

AU - Bowman, Gene

AU - Popp, Julius

PY - 2019/3/1

Y1 - 2019/3/1

N2 - The systems-level relationship between the proteomes of cerebrospinal fluid (CSF) and plasma has not been comprehensively described so far. Recently developed shotgun proteomic workflows allow for deeper characterization of the proteomes from body fluids in much larger sample size. We deployed state-of-the-art mass spectrometry-based proteomics in paired CSF and plasma samples volunteered by 120 elders with and without cognitive impairment to comprehensively characterize and examine compartmental proteome differences and relationships between both body fluids. We further assessed the influence of blood-brain barrier (BBB) integrity and tested the hypothesis that BBB breakdown can be identified from CSF and plasma proteome alterations in nondemented elders. We quantified 790 proteins in CSF and 422 proteins in plasma, and 255 of the proteins were identified in both compartments. Pearson's statistics determined 28 proteins with associated levels between CSF and plasma. BBB integrity as defined with the CSF/serum albumin index influenced 76 CSF/plasma protein ratios. In least absolute shrinkage and selection operator models, CSF and plasma proteins improved identification of BBB impairment. In conclusion, we provide here a first comprehensive draft map of interacting human CSF and plasma proteomes, in view of their complex and dynamic compositions, and influence of the BBB.

AB - The systems-level relationship between the proteomes of cerebrospinal fluid (CSF) and plasma has not been comprehensively described so far. Recently developed shotgun proteomic workflows allow for deeper characterization of the proteomes from body fluids in much larger sample size. We deployed state-of-the-art mass spectrometry-based proteomics in paired CSF and plasma samples volunteered by 120 elders with and without cognitive impairment to comprehensively characterize and examine compartmental proteome differences and relationships between both body fluids. We further assessed the influence of blood-brain barrier (BBB) integrity and tested the hypothesis that BBB breakdown can be identified from CSF and plasma proteome alterations in nondemented elders. We quantified 790 proteins in CSF and 422 proteins in plasma, and 255 of the proteins were identified in both compartments. Pearson's statistics determined 28 proteins with associated levels between CSF and plasma. BBB integrity as defined with the CSF/serum albumin index influenced 76 CSF/plasma protein ratios. In least absolute shrinkage and selection operator models, CSF and plasma proteins improved identification of BBB impairment. In conclusion, we provide here a first comprehensive draft map of interacting human CSF and plasma proteomes, in view of their complex and dynamic compositions, and influence of the BBB.

KW - BBB

KW - Blood

KW - Brain

KW - Cerebrospinal fluid

KW - Circulation

KW - Clinical proteomics

KW - CSF

KW - Mass spectrometry

KW - Nervous system

KW - Serum/plasma

UR - http://www.scopus.com/inward/record.url?scp=85062376140&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85062376140&partnerID=8YFLogxK

U2 - 10.1021/acs.jproteome.8b00809

DO - 10.1021/acs.jproteome.8b00809

M3 - Article

C2 - 30702894

AN - SCOPUS:85062376140

VL - 18

SP - 1162

EP - 1174

JO - Journal of Proteome Research

JF - Journal of Proteome Research

SN - 1535-3893

IS - 3

ER -