Proteolytically-induced precipitation in mouse lens crystallins

M. Azuma, C. Fukiage, Y. Nakamura, Y. Tamada, Thomas (Tom) Shearer

Research output: Contribution to journalArticle

Abstract

Purpose. We previously demonst-ated that crystallins from young rats, but not from bovine, chick ot human, showed proteolytic insolubiuzatio However, nc data have directly tested if crystallm fragmerits from r:. dent lenses other than rat, become insoluble. Thus, the purposes ui inu experiment were to determine if cr/stallins from another dent, mouse, were susceptible to calpain-indud precipitation and if aging in mice abolished light scattering. Methods. Total soluble proteins from mouse lenses were proteolyzed by endogenous or exogenous m-calpain. Subsequent precipitation of crystall ns was measured by light scattering. SDS-PAGE and immunoblots fcr calpain assessed proteolysis of crystallins and activation of calpain Results. Total soluble proteins from young mouse showed massive light scattering, and this light scattering was associated with proteolysis of crystallins. Calpain molecule in incubation mixture decreased. This was further evidence for activation and subsequent degradation of m-calpain. The cause of the light scattering was due to activation of m-calpain since E64 inhibitor attenuated light scattering. Tola! so uble proteins from old mouse lenses did not show calpain-induced lig it scattering. Conclusions. These results suggested that prevention of light scattering during aging of mouse lenses may be loss in calpain activit/ and/or lack of protein susceptibility.

Original languageEnglish (US)
JournalInvestigative Ophthalmology and Visual Science
Volume38
Issue number4
StatePublished - 1997
Externally publishedYes

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Crystallins
Calpain
Lenses
Light
Proteolysis
Proteins
Polyacrylamide Gel Electrophoresis
m-calpain

ASJC Scopus subject areas

  • Ophthalmology

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Proteolytically-induced precipitation in mouse lens crystallins. / Azuma, M.; Fukiage, C.; Nakamura, Y.; Tamada, Y.; Shearer, Thomas (Tom).

In: Investigative Ophthalmology and Visual Science, Vol. 38, No. 4, 1997.

Research output: Contribution to journalArticle

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AU - Azuma, M.

AU - Fukiage, C.

AU - Nakamura, Y.

AU - Tamada, Y.

AU - Shearer, Thomas (Tom)

PY - 1997

Y1 - 1997

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