Proteolytic changes in main intrinsic polypeptide (MIP26) from membranes in selenite cataract

L. L. David, L. J. Takemoto, R. S. Anderson, T. R. Shearer

Research output: Contribution to journalArticlepeer-review

18 Scopus citations

Abstract

Experimental nuclear cataract produced by an overdose of sodium selenite exhibited limited proteolysis, including breakdown of main intrinsic polypeptide (MIP26) to 24 and 22 kD fragments (1). Micro-sequencing and site specific immunologic probes were used in the present study to determine regions of cleavage in MIP26 during selenite cataractogenesis. Data suggested that proteolysis occurred in the C-terminus of MIP26. This may have lead to exposure of normally hidden amino acid residues on the C-terminal extension of MIP26. Loss of antigenicity of the N-terminus occurred. These significant changes to the MIP26 molecule might adversely affect communication between lens fiber cells and contribute to selenite cataract.

Original languageEnglish (US)
Pages (from-to)411-417
Number of pages7
JournalCurrent Eye Research
Volume7
Issue number4
DOIs
StatePublished - 1988

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

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