Proteins of the postsynaptic density

Gary Banker, L. Churchill, C. W. Cotman

Research output: Contribution to journalArticle

78 Citations (Scopus)

Abstract

An analysis was made of the protein composition of a fraction of postsynaptic densities (PSDs) prepared from rat brain. Protein makes up 90% of the material in the PSD fraction. Two major polypeptide fractions are present, based on sodium dodecyl sulfate polyacrylamide gel electrophoresis. The major polypeptide fraction has a molecular weight of 53,000, makes up about 45% of the PSD protein, and comigrates on gels with a major polypeptide of the synaptic plasma membrane. The other polypeptide band has a molecular weight of 97,000, accounts for 17% of the PSD protein, and is not a prominent constituent of other fractions. Six other polypeptides of higher molecular weight (100,000 to 180,000) are consistently present in small amounts (3 to 9% each). The PSD fraction contains slightly greater amounts of polar amino acids and proline than the synaptic plasma membrane fraction, but no amino acid is usually prominent. The PSD apparently consists of a structural matrix formed primarily by a single polypeptide or class of polypeptides of 53,000 molecular weight. Small amounts of other specialized proteins are contained within this matrix.

Original languageEnglish (US)
JournalJournal of Cell Biology
Volume63
Issue number2
StatePublished - 1974
Externally publishedYes

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Post-Synaptic Density
Peptides
Molecular Weight
Synaptic Membranes
Cell Membrane
Amino Acids
Proteins
postsynaptic density proteins
Proline
Sodium Dodecyl Sulfate
Polyacrylamide Gel Electrophoresis
Gels
Brain

ASJC Scopus subject areas

  • Cell Biology

Cite this

Banker, G., Churchill, L., & Cotman, C. W. (1974). Proteins of the postsynaptic density. Journal of Cell Biology, 63(2).

Proteins of the postsynaptic density. / Banker, Gary; Churchill, L.; Cotman, C. W.

In: Journal of Cell Biology, Vol. 63, No. 2, 1974.

Research output: Contribution to journalArticle

Banker, G, Churchill, L & Cotman, CW 1974, 'Proteins of the postsynaptic density', Journal of Cell Biology, vol. 63, no. 2.
Banker G, Churchill L, Cotman CW. Proteins of the postsynaptic density. Journal of Cell Biology. 1974;63(2).
Banker, Gary ; Churchill, L. ; Cotman, C. W. / Proteins of the postsynaptic density. In: Journal of Cell Biology. 1974 ; Vol. 63, No. 2.
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