Protein synthesis and processing in cytoplasmic microdomains beneath postsynaptic sites on CNS neurons - A mechanism for establishing and maintaining a mosaic postsynaptic receptive surface

Oswald Steward, Lauren Davis, Carlos Dotti, Linda L. Phillips, Anuradha Rao, Gary Banker

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

Recent studies have shown that protein synthetic machinery consisting of polyribosomes and associated membranous cisterns is selectively localized beneath synaptic sites on neurons. In the present paper, the role of this machinery in neuronal function will be considered. We will: 1. Summarize the studies that characterize the polyribosomes and define their associations with membranous cisterns. Taken together, these observations suggest the existence of a system for the synthesis and posttranslational processing of proteins at individual synaptic sites; 2. Review the evidence that the protein synthetic machinery is particularly prominent during the initial formation of synaptic contacts (during early development), and during lesion-induced synaptogenesis in mature animals. These observations have led to the hypothesis that the polyribosomes produce proteins that play a role in the formation of the synaptic junction; 3. Review evidence that supports the hypothesis that there is a local synthesis of protein within dendrites, as well as local glycosylation; 4. Describe the evidence suggesting that at least some of the protein constituents of the synaptic junction itself are synthesized locally; and 5. Descibe our studies that reveal a mechanism for selective dendritic transport of RNA; this transport mechanism permits the delivery of RNA to postsynaptic sites throughout the dendritic arbor. We will advance the hypothesis that neurons position protein synthetic machinery together with the mRNA's that are appropriate for particular synapses beneath synaptic contact regions. At the synaptic site, this machinery could then direct the synthesis of particular proteins that are critical for synapse formation or maintenance. The positioning of protein synthetic machinery at postsynaptic sites permits a rapid local regulation of the production of key proteins by events at individual synapses.

Original languageEnglish (US)
Pages (from-to)227-261
Number of pages35
JournalMolecular Neurobiology
Volume2
Issue number4
DOIs
StatePublished - Dec 1988
Externally publishedYes

Fingerprint

Neurons
Proteins
Polyribosomes
Synapses
RNA Transport
Post Translational Protein Processing
Dendrites
Glycosylation
Maintenance
RNA
Messenger RNA

Keywords

  • dendrites
  • Polyribosomes
  • protein synthetic machinery
  • RNA, dendritic transport of
  • synaptic junction
  • synaptogenesis

ASJC Scopus subject areas

  • Neuroscience(all)
  • Physiology
  • Cellular and Molecular Neuroscience

Cite this

Protein synthesis and processing in cytoplasmic microdomains beneath postsynaptic sites on CNS neurons - A mechanism for establishing and maintaining a mosaic postsynaptic receptive surface. / Steward, Oswald; Davis, Lauren; Dotti, Carlos; Phillips, Linda L.; Rao, Anuradha; Banker, Gary.

In: Molecular Neurobiology, Vol. 2, No. 4, 12.1988, p. 227-261.

Research output: Contribution to journalArticle

Steward, Oswald ; Davis, Lauren ; Dotti, Carlos ; Phillips, Linda L. ; Rao, Anuradha ; Banker, Gary. / Protein synthesis and processing in cytoplasmic microdomains beneath postsynaptic sites on CNS neurons - A mechanism for establishing and maintaining a mosaic postsynaptic receptive surface. In: Molecular Neurobiology. 1988 ; Vol. 2, No. 4. pp. 227-261.
@article{952a3a5fa36f47bc9bfd918dad857028,
title = "Protein synthesis and processing in cytoplasmic microdomains beneath postsynaptic sites on CNS neurons - A mechanism for establishing and maintaining a mosaic postsynaptic receptive surface",
abstract = "Recent studies have shown that protein synthetic machinery consisting of polyribosomes and associated membranous cisterns is selectively localized beneath synaptic sites on neurons. In the present paper, the role of this machinery in neuronal function will be considered. We will: 1. Summarize the studies that characterize the polyribosomes and define their associations with membranous cisterns. Taken together, these observations suggest the existence of a system for the synthesis and posttranslational processing of proteins at individual synaptic sites; 2. Review the evidence that the protein synthetic machinery is particularly prominent during the initial formation of synaptic contacts (during early development), and during lesion-induced synaptogenesis in mature animals. These observations have led to the hypothesis that the polyribosomes produce proteins that play a role in the formation of the synaptic junction; 3. Review evidence that supports the hypothesis that there is a local synthesis of protein within dendrites, as well as local glycosylation; 4. Describe the evidence suggesting that at least some of the protein constituents of the synaptic junction itself are synthesized locally; and 5. Descibe our studies that reveal a mechanism for selective dendritic transport of RNA; this transport mechanism permits the delivery of RNA to postsynaptic sites throughout the dendritic arbor. We will advance the hypothesis that neurons position protein synthetic machinery together with the mRNA's that are appropriate for particular synapses beneath synaptic contact regions. At the synaptic site, this machinery could then direct the synthesis of particular proteins that are critical for synapse formation or maintenance. The positioning of protein synthetic machinery at postsynaptic sites permits a rapid local regulation of the production of key proteins by events at individual synapses.",
keywords = "dendrites, Polyribosomes, protein synthetic machinery, RNA, dendritic transport of, synaptic junction, synaptogenesis",
author = "Oswald Steward and Lauren Davis and Carlos Dotti and Phillips, {Linda L.} and Anuradha Rao and Gary Banker",
year = "1988",
month = "12",
doi = "10.1007/BF02935634",
language = "English (US)",
volume = "2",
pages = "227--261",
journal = "Molecular Neurobiology",
issn = "0893-7648",
publisher = "Humana Press",
number = "4",

}

TY - JOUR

T1 - Protein synthesis and processing in cytoplasmic microdomains beneath postsynaptic sites on CNS neurons - A mechanism for establishing and maintaining a mosaic postsynaptic receptive surface

AU - Steward, Oswald

AU - Davis, Lauren

AU - Dotti, Carlos

AU - Phillips, Linda L.

AU - Rao, Anuradha

AU - Banker, Gary

PY - 1988/12

Y1 - 1988/12

N2 - Recent studies have shown that protein synthetic machinery consisting of polyribosomes and associated membranous cisterns is selectively localized beneath synaptic sites on neurons. In the present paper, the role of this machinery in neuronal function will be considered. We will: 1. Summarize the studies that characterize the polyribosomes and define their associations with membranous cisterns. Taken together, these observations suggest the existence of a system for the synthesis and posttranslational processing of proteins at individual synaptic sites; 2. Review the evidence that the protein synthetic machinery is particularly prominent during the initial formation of synaptic contacts (during early development), and during lesion-induced synaptogenesis in mature animals. These observations have led to the hypothesis that the polyribosomes produce proteins that play a role in the formation of the synaptic junction; 3. Review evidence that supports the hypothesis that there is a local synthesis of protein within dendrites, as well as local glycosylation; 4. Describe the evidence suggesting that at least some of the protein constituents of the synaptic junction itself are synthesized locally; and 5. Descibe our studies that reveal a mechanism for selective dendritic transport of RNA; this transport mechanism permits the delivery of RNA to postsynaptic sites throughout the dendritic arbor. We will advance the hypothesis that neurons position protein synthetic machinery together with the mRNA's that are appropriate for particular synapses beneath synaptic contact regions. At the synaptic site, this machinery could then direct the synthesis of particular proteins that are critical for synapse formation or maintenance. The positioning of protein synthetic machinery at postsynaptic sites permits a rapid local regulation of the production of key proteins by events at individual synapses.

AB - Recent studies have shown that protein synthetic machinery consisting of polyribosomes and associated membranous cisterns is selectively localized beneath synaptic sites on neurons. In the present paper, the role of this machinery in neuronal function will be considered. We will: 1. Summarize the studies that characterize the polyribosomes and define their associations with membranous cisterns. Taken together, these observations suggest the existence of a system for the synthesis and posttranslational processing of proteins at individual synaptic sites; 2. Review the evidence that the protein synthetic machinery is particularly prominent during the initial formation of synaptic contacts (during early development), and during lesion-induced synaptogenesis in mature animals. These observations have led to the hypothesis that the polyribosomes produce proteins that play a role in the formation of the synaptic junction; 3. Review evidence that supports the hypothesis that there is a local synthesis of protein within dendrites, as well as local glycosylation; 4. Describe the evidence suggesting that at least some of the protein constituents of the synaptic junction itself are synthesized locally; and 5. Descibe our studies that reveal a mechanism for selective dendritic transport of RNA; this transport mechanism permits the delivery of RNA to postsynaptic sites throughout the dendritic arbor. We will advance the hypothesis that neurons position protein synthetic machinery together with the mRNA's that are appropriate for particular synapses beneath synaptic contact regions. At the synaptic site, this machinery could then direct the synthesis of particular proteins that are critical for synapse formation or maintenance. The positioning of protein synthetic machinery at postsynaptic sites permits a rapid local regulation of the production of key proteins by events at individual synapses.

KW - dendrites

KW - Polyribosomes

KW - protein synthetic machinery

KW - RNA, dendritic transport of

KW - synaptic junction

KW - synaptogenesis

UR - http://www.scopus.com/inward/record.url?scp=0024153232&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024153232&partnerID=8YFLogxK

U2 - 10.1007/BF02935634

DO - 10.1007/BF02935634

M3 - Article

VL - 2

SP - 227

EP - 261

JO - Molecular Neurobiology

JF - Molecular Neurobiology

SN - 0893-7648

IS - 4

ER -