Protein phosphatase 2a is associated with class c l-type calcium channels (Cav1.2) and Antagonizes channel phosphorylation by cAMP-dependent protein kinase

Monika Davare, Mary C. Horne, Johannes W. Hell

Research output: Contribution to journalArticle

135 Citations (Scopus)

Abstract

Phosphorylation by cAMP-dependent protein kinase (PKA) regulates a vast number of cellular functions. An important target for PKA in brain and heart is the class C L-type Ca2+ channel (Cav1.2). PKA phosphorylates serine 1928 in the central, pore-forming α1c subunit of this channel. Regulation of channel activity by PKA requires a proper balance between phosphorylation and dephosphorylation. For fast and specific signaling, PKA is recruited to this channel by an protein kinase A anchor protein (Davare, M. A., Dong, F., Rubin, C. S., and Hell, J. W. (1999) J. Biol. Chem. 274, 30280-30287). A phosphatase may be associated with the channel to effectively balance serine 1928 phosphorylation by channel-bound PKA. Dephosphorylation of this site is mediated by a serine/threonine phosphatase that is inhibited by okadaic acid and microcystin. We show that immunoprecipitation of the channel complex from rat brain results in coprecipitation of PP2A. Stoichiometric analysis indicates that about 80% of the channel complexes contain PP2A. PP2A directly and stably binds to the C-terminal 557 amino acids of α1c. This interaction does not depend on serine 1928 phosphorylation and is not altered by PP2A catalytic site inhibitors. These results indicate that the PP2A-α1C interaction constitutively recruits PP2A to the channel complex rather than being a transient substrate-catalytic site interaction. Functional as-says with the immunoisolated class C channel complex showed that channel-associated PP2A effectively reverses serine 1928 phosphorylation by endogenous PKA. Our findings demonstrate that both PKA and PP2A are integral components of the class C L-type Ca2+channel that determine the phosphorylation level of serine 1928 and thereby channel activity.

Original languageEnglish (US)
Pages (from-to)39710-39717
Number of pages8
JournalJournal of Biological Chemistry
Volume275
Issue number50
DOIs
StatePublished - Dec 15 2000
Externally publishedYes

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Protein Phosphatase 2
Phosphorylation
Cyclic AMP-Dependent Protein Kinases
Protein Kinases
Serine
A Kinase Anchor Proteins
Brain
Catalytic Domain
Okadaic Acid
Phosphoprotein Phosphatases
Protein-Serine-Threonine Kinases
Coprecipitation
Phosphoric Monoester Hydrolases
Immunoprecipitation
L-type calcium channel alpha(1C)
Rats
Amino Acids
Substrates

ASJC Scopus subject areas

  • Biochemistry

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Protein phosphatase 2a is associated with class c l-type calcium channels (Cav1.2) and Antagonizes channel phosphorylation by cAMP-dependent protein kinase. / Davare, Monika; Horne, Mary C.; Hell, Johannes W.

In: Journal of Biological Chemistry, Vol. 275, No. 50, 15.12.2000, p. 39710-39717.

Research output: Contribution to journalArticle

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