Protein kinase CK2 is coassembled with small conductance Ca 2+-activated K+ channels and regulates channel gating

Wolfgang Bildl, Tim Strassmaier, Henrike Thurm, Jens Andersen, Silke Eble, Dominik Oliver, Marlies Knipper, Matthias Mann, Uwe Schulte, John Adelman, Bernd Fakler

Research output: Contribution to journalArticle

134 Citations (Scopus)

Abstract

Small conductance Ca2+-activated K+ channels (SK channels) couple the membrane potential to fluctuations in intracellular Ca 2+ concentration in many types of cells. SK channels are gated by Ca2+ ions via calmodulin that is constitutively bound to the intracellular C terminus of the channels and serves as the Ca2+ sensor. Here we show that, in addition, the cytoplasmic N and C termini of the channel protein form a polyprotein complex with the catalytic and regulatory subunits of protein kinase CK2 and protein phosphatase 2A. Within this complex, CK2 phosphorylates calmodulin at threonine 80, reducing by 5-fold the apparent Ca2+ sensitivity and accelerating channel deactivation. The results show that native SK channels are polyprotein complexes and demonstrate that the balance between kinase and phosphatase activities within the protein complex shapes the hyperpolarizing response mediated by SK channels.

Original languageEnglish (US)
Pages (from-to)847-858
Number of pages12
JournalNeuron
Volume43
Issue number6
DOIs
StatePublished - Sep 16 2004

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Casein Kinase II
Calcium-Activated Potassium Channels
Polyproteins
Calmodulin
Protein Phosphatase 2
Threonine
Phosphoric Monoester Hydrolases
Membrane Potentials
Catalytic Domain
Proteins
Phosphotransferases
Ions

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Bildl, W., Strassmaier, T., Thurm, H., Andersen, J., Eble, S., Oliver, D., ... Fakler, B. (2004). Protein kinase CK2 is coassembled with small conductance Ca 2+-activated K+ channels and regulates channel gating. Neuron, 43(6), 847-858. https://doi.org/10.1016/j.neuron.2004.08.033

Protein kinase CK2 is coassembled with small conductance Ca 2+-activated K+ channels and regulates channel gating. / Bildl, Wolfgang; Strassmaier, Tim; Thurm, Henrike; Andersen, Jens; Eble, Silke; Oliver, Dominik; Knipper, Marlies; Mann, Matthias; Schulte, Uwe; Adelman, John; Fakler, Bernd.

In: Neuron, Vol. 43, No. 6, 16.09.2004, p. 847-858.

Research output: Contribution to journalArticle

Bildl, W, Strassmaier, T, Thurm, H, Andersen, J, Eble, S, Oliver, D, Knipper, M, Mann, M, Schulte, U, Adelman, J & Fakler, B 2004, 'Protein kinase CK2 is coassembled with small conductance Ca 2+-activated K+ channels and regulates channel gating', Neuron, vol. 43, no. 6, pp. 847-858. https://doi.org/10.1016/j.neuron.2004.08.033
Bildl, Wolfgang ; Strassmaier, Tim ; Thurm, Henrike ; Andersen, Jens ; Eble, Silke ; Oliver, Dominik ; Knipper, Marlies ; Mann, Matthias ; Schulte, Uwe ; Adelman, John ; Fakler, Bernd. / Protein kinase CK2 is coassembled with small conductance Ca 2+-activated K+ channels and regulates channel gating. In: Neuron. 2004 ; Vol. 43, No. 6. pp. 847-858.
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