Abstract
Kupffer cells play an important role in sepsis-mediated liver injury. We tested the hypothesis that PKC-ζ plays a critical role in Kupffer cell apoptosis during sepsis. Sepsis was induced in rats by cecal ligation and puncture (CLP); 12 h later, livers were assayed for PKC-ζ, IKKα, IKKβ, IKKγ, NF-κB, Fas/FasL, Caspase-3, and DNA fragmentation. Kupffer cells from control rats were infected with AdPKC-ζ DN to inhibit PKC-ζ, or transfected with pCMVPKC-ζ to overexpress PKC-ζ, and then treated with lipopolysaccharide (LPS). Cellular extracts were assayed for PKC-ζ, IKKα, IKKβ, IKKγ, NF-κB, Fas/FasL, Caspase-3, and DNA fragmentation. During sepsis, PKC-ζ localized in cells positive for the macrophage marker (F4/80). CLP upregulated PKC-ζ protein and activity, IKKβ, IKKγ, NF-κB, Fas/FasL, Caspase-3, and increased DNA fragmentation in rat livers (all p∈
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1712-1721 |
| Number of pages | 10 |
| Journal | Journal of Gastrointestinal Surgery |
| Volume | 11 |
| Issue number | 12 |
| DOIs | |
| State | Published - Dec 2007 |
| Externally published | Yes |
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Keywords
- Apoptosis
- Kupffer cells
- Liver injury
- PKC-ζ
- Sepsis
ASJC Scopus subject areas
- Surgery
Cite this
Protein kinase C-zeta (PKC-ζ) regulates Kupffer cell apoptosis during experimental sepsis. / Peng, Yanhua; Sigua, Celia A.; Karsonovich, Cynthia; Murr, Michel M.
In: Journal of Gastrointestinal Surgery, Vol. 11, No. 12, 12.2007, p. 1712-1721.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Protein kinase C-zeta (PKC-ζ) regulates Kupffer cell apoptosis during experimental sepsis
AU - Peng, Yanhua
AU - Sigua, Celia A.
AU - Karsonovich, Cynthia
AU - Murr, Michel M.
PY - 2007/12
Y1 - 2007/12
N2 - Kupffer cells play an important role in sepsis-mediated liver injury. We tested the hypothesis that PKC-ζ plays a critical role in Kupffer cell apoptosis during sepsis. Sepsis was induced in rats by cecal ligation and puncture (CLP); 12 h later, livers were assayed for PKC-ζ, IKKα, IKKβ, IKKγ, NF-κB, Fas/FasL, Caspase-3, and DNA fragmentation. Kupffer cells from control rats were infected with AdPKC-ζ DN to inhibit PKC-ζ, or transfected with pCMVPKC-ζ to overexpress PKC-ζ, and then treated with lipopolysaccharide (LPS). Cellular extracts were assayed for PKC-ζ, IKKα, IKKβ, IKKγ, NF-κB, Fas/FasL, Caspase-3, and DNA fragmentation. During sepsis, PKC-ζ localized in cells positive for the macrophage marker (F4/80). CLP upregulated PKC-ζ protein and activity, IKKβ, IKKγ, NF-κB, Fas/FasL, Caspase-3, and increased DNA fragmentation in rat livers (all p∈
AB - Kupffer cells play an important role in sepsis-mediated liver injury. We tested the hypothesis that PKC-ζ plays a critical role in Kupffer cell apoptosis during sepsis. Sepsis was induced in rats by cecal ligation and puncture (CLP); 12 h later, livers were assayed for PKC-ζ, IKKα, IKKβ, IKKγ, NF-κB, Fas/FasL, Caspase-3, and DNA fragmentation. Kupffer cells from control rats were infected with AdPKC-ζ DN to inhibit PKC-ζ, or transfected with pCMVPKC-ζ to overexpress PKC-ζ, and then treated with lipopolysaccharide (LPS). Cellular extracts were assayed for PKC-ζ, IKKα, IKKβ, IKKγ, NF-κB, Fas/FasL, Caspase-3, and DNA fragmentation. During sepsis, PKC-ζ localized in cells positive for the macrophage marker (F4/80). CLP upregulated PKC-ζ protein and activity, IKKβ, IKKγ, NF-κB, Fas/FasL, Caspase-3, and increased DNA fragmentation in rat livers (all p∈
KW - Apoptosis
KW - Kupffer cells
KW - Liver injury
KW - PKC-ζ
KW - Sepsis
UR - http://www.scopus.com/inward/record.url?scp=36148993630&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=36148993630&partnerID=8YFLogxK
U2 - 10.1007/s11605-007-0314-9
DO - 10.1007/s11605-007-0314-9
M3 - Article
C2 - 17899301
AN - SCOPUS:36148993630
VL - 11
SP - 1712
EP - 1721
JO - Journal of Gastrointestinal Surgery
JF - Journal of Gastrointestinal Surgery
SN - 1091-255X
IS - 12
ER -