Protein dynamics during seed germination under copper stress in Arabidopsis over-expressing Potentilla superoxide dismutase

Tejpal Gill, Vivek Dogra, Sanjay Kumar, Paramvir Singh Ahuja, Yelam Sreenivasulu

Research output: Contribution to journalArticlepeer-review

30 Scopus citations

Abstract

Copper (Cu), though an essential micronutrient for plants, poses toxicity at higher concentrations possibly by inducing oxidative stress. With the background that enzyme superoxide dismutase (SOD) ameliorates oxidative stress, the present work focused on understanding physiological and proteomic response of Arabidopsis seeds constitutively over-expressing copper-zinc SOD of Potentilla atrosanguinea (PaSOD) during germination in response to varied concentrations of copper sulphate (Cu stress). Transgenics showed higher germination percentage and required less "mean time to germination" under Cu-stress. In response to Cu stress, 39 differentially expressed protein spots were detected by 2-D electrophoresis in proteins of germinating wild type (WT) and transgenic seeds, of which 14 spots appeared exclusively in transgenics. Among the rest 25 protein spots, 14 showed down-regulation, one showed up-regulation, and 10 spots disappeared. MALDI-TOF and subsequent peptide mass fingerprinting analysis revealed that the down-regulated proteins in transgenics were related to oxidative stress, detoxification, germination, intermediary metabolism and regulatory proteins. Up-regulated proteins in WT and down-regulated proteins in transgenic during Cu stress were the same. Changes in key proteins, vis-à-vis alleviation of oxidative stress in transgenic Arabidopsis over-expressing PaSOD possibly alleviated toxicity of Cu-induced stress during seed germination, resulting in higher germination rate and germination percentage.

Original languageEnglish (US)
Pages (from-to)165-172
Number of pages8
JournalJournal of Plant Research
Volume125
Issue number1
DOIs
StatePublished - Jan 2012
Externally publishedYes

Keywords

  • Arabidopsis thaliana
  • Heavy metal toxicity
  • Oxidative stress
  • Seed germination proteins
  • SOD
  • Transgenics

ASJC Scopus subject areas

  • Plant Science

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