Protein binding to the interferon response enhancer correlates with interferon induction of 2′-5′-oligoadenylate synthetase in normal and interferon-resistant Friend cells

Eliana M. Coccia, Daniel Vaiman, Jacob Raber, Giovanna Marziali, Gianna Fiorucci, Roberto Orsatti, Batya Cohen, Nili Nissim, Giovanna Romeo, Elisabetta Aff Abris, Judith Chebath, Angela Battistini

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The induction of transcription of the 2prime;-5prime-oIigoadenylate (2-5A) synthetase gene by type I (α/β) and type II (γ) interferons (IFNs) has been studied in wild-type (w.t.) and IFN-resistant Friend leukemia cells (FLC). Following IFN treatment, new complexes are formed in vitro between the IFN-responsive sequence (IRS) of the 2-5A synthetase gene and cellular proteins. Within minutes after IFN-α/β addition to w.t. FLC, an IRS-protein complex, designated F1, is detected, as already observed in several human cell lines. In response to IFN-γ, a novel complex, designated Fg, is observed in w.t. FLC. The Fg complex appears within 3 h, while an F1-like complex is faintly visible 10 to 24 h later. In the IFN-α/β-resistant FLC, IFN-γ induces only the Fg complex and fails to induce F1. Fg formation is correlated with the IFN-γ-induced transcription of the 2-5A synthetase gene and the appearance of the corresponding enzymatic activity in both w.t. and IFN-α/β-resistant FLC. These findings suggest that F1 and Fg represent two distinct effector complexes by which type I and type II IFNs, respectively, induce 2-5A synthetase.

Original languageEnglish (US)
Pages (from-to)2081-2087
Number of pages7
JournalJournal of Virology
Volume65
Issue number4
StatePublished - 1991
Externally publishedYes

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protein binding
interferons
Ligases
ligases
Protein Binding
Interferons
2',5'-Oligoadenylate Synthetase
leukemia
Leukemia
cells
Interferon Type I
interferon-gamma
Interferon-gamma
transcription (genetics)
2',5'-oligoadenylate
human cell lines
genes
Genes
Proteins
amino acid sequences

ASJC Scopus subject areas

  • Immunology

Cite this

Protein binding to the interferon response enhancer correlates with interferon induction of 2′-5′-oligoadenylate synthetase in normal and interferon-resistant Friend cells. / Coccia, Eliana M.; Vaiman, Daniel; Raber, Jacob; Marziali, Giovanna; Fiorucci, Gianna; Orsatti, Roberto; Cohen, Batya; Nissim, Nili; Romeo, Giovanna; Abris, Elisabetta Aff; Chebath, Judith; Battistini, Angela.

In: Journal of Virology, Vol. 65, No. 4, 1991, p. 2081-2087.

Research output: Contribution to journalArticle

Coccia, EM, Vaiman, D, Raber, J, Marziali, G, Fiorucci, G, Orsatti, R, Cohen, B, Nissim, N, Romeo, G, Abris, EA, Chebath, J & Battistini, A 1991, 'Protein binding to the interferon response enhancer correlates with interferon induction of 2′-5′-oligoadenylate synthetase in normal and interferon-resistant Friend cells', Journal of Virology, vol. 65, no. 4, pp. 2081-2087.
Coccia, Eliana M. ; Vaiman, Daniel ; Raber, Jacob ; Marziali, Giovanna ; Fiorucci, Gianna ; Orsatti, Roberto ; Cohen, Batya ; Nissim, Nili ; Romeo, Giovanna ; Abris, Elisabetta Aff ; Chebath, Judith ; Battistini, Angela. / Protein binding to the interferon response enhancer correlates with interferon induction of 2′-5′-oligoadenylate synthetase in normal and interferon-resistant Friend cells. In: Journal of Virology. 1991 ; Vol. 65, No. 4. pp. 2081-2087.
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abstract = "The induction of transcription of the 2prime;-5prime-oIigoadenylate (2-5A) synthetase gene by type I (α/β) and type II (γ) interferons (IFNs) has been studied in wild-type (w.t.) and IFN-resistant Friend leukemia cells (FLC). Following IFN treatment, new complexes are formed in vitro between the IFN-responsive sequence (IRS) of the 2-5A synthetase gene and cellular proteins. Within minutes after IFN-α/β addition to w.t. FLC, an IRS-protein complex, designated F1, is detected, as already observed in several human cell lines. In response to IFN-γ, a novel complex, designated Fg, is observed in w.t. FLC. The Fg complex appears within 3 h, while an F1-like complex is faintly visible 10 to 24 h later. In the IFN-α/β-resistant FLC, IFN-γ induces only the Fg complex and fails to induce F1. Fg formation is correlated with the IFN-γ-induced transcription of the 2-5A synthetase gene and the appearance of the corresponding enzymatic activity in both w.t. and IFN-α/β-resistant FLC. These findings suggest that F1 and Fg represent two distinct effector complexes by which type I and type II IFNs, respectively, induce 2-5A synthetase.",
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