TY - JOUR
T1 - Protective effects of memantine against ischemia-reperfusion injury in spontaneously hypertensive rats
AU - Dogan, A.
AU - Eras, M. A.
AU - Raghavendra Rao, V. L.
AU - Dempsey, Robert J.
PY - 1999
Y1 - 1999
N2 - Memantine, an uncompetitive NMDA open-channel blocker, has been shown to be effective in preventing neuronal damage after permanent focal cerebral ischemia. Reperfusion after a long period of ischemia may aggravate the progression of neuronal damage. Those drugs that show protective effects after permanent cerebral ischemia, therefore, might fail to do so against ischemia-reperfusion injury. In this study we evaluated the effects of memantine on brain edema formation and ischemic injury volume after transient cerebral ischemia. Male Spontaneously Hypertensive Rats (SHR) weighing 250-300 g were anesthetized with halothane and subjected to 1 hour of temporary middle cerebral artery occlusion by an intraluminal suture. 20 mg/kg of memantine or saline were injected intraperitoneally 5 min. after the induction of ischemia. Physiological parameters and regional cerebral blood flow were monitored during the surgical procedure. Brain water content and ischemic injury volume were measured with the wet dry method and 2,3,5-triphenyl tetrazolium chloride monohydrate (TTC) staining, respectively, at 24 hours after occlusion. There were no statistically significant differences between the groups regarding physiological parameters during the procedure. Memantine treatment (n = 9) reduced the brain water content significantly in the cortex compared to saline treatment (n = 8; 83.1 ± 0.7% vs. 84.5 ± 1.5%, respectively, p < 0.05). The total volume of ischemic brain injury was 300 ± 49 mm3 in the animals treated with saline (n = 13). Treatment with 20 mg/kg memantine (n = 14) reduced the ischemic injury volume to 233 ± 61 mm3 (P < 0.01). These results demonstrate that the harmful effects of recirculation after a period of ischemia can be attenuated by the treatment of memantine, perhaps by its action at the NMDA receptors.
AB - Memantine, an uncompetitive NMDA open-channel blocker, has been shown to be effective in preventing neuronal damage after permanent focal cerebral ischemia. Reperfusion after a long period of ischemia may aggravate the progression of neuronal damage. Those drugs that show protective effects after permanent cerebral ischemia, therefore, might fail to do so against ischemia-reperfusion injury. In this study we evaluated the effects of memantine on brain edema formation and ischemic injury volume after transient cerebral ischemia. Male Spontaneously Hypertensive Rats (SHR) weighing 250-300 g were anesthetized with halothane and subjected to 1 hour of temporary middle cerebral artery occlusion by an intraluminal suture. 20 mg/kg of memantine or saline were injected intraperitoneally 5 min. after the induction of ischemia. Physiological parameters and regional cerebral blood flow were monitored during the surgical procedure. Brain water content and ischemic injury volume were measured with the wet dry method and 2,3,5-triphenyl tetrazolium chloride monohydrate (TTC) staining, respectively, at 24 hours after occlusion. There were no statistically significant differences between the groups regarding physiological parameters during the procedure. Memantine treatment (n = 9) reduced the brain water content significantly in the cortex compared to saline treatment (n = 8; 83.1 ± 0.7% vs. 84.5 ± 1.5%, respectively, p < 0.05). The total volume of ischemic brain injury was 300 ± 49 mm3 in the animals treated with saline (n = 13). Treatment with 20 mg/kg memantine (n = 14) reduced the ischemic injury volume to 233 ± 61 mm3 (P < 0.01). These results demonstrate that the harmful effects of recirculation after a period of ischemia can be attenuated by the treatment of memantine, perhaps by its action at the NMDA receptors.
KW - Cerebral edema
KW - Memantine
KW - NMDA receptor
KW - Transient cerebral ischemia
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U2 - 10.1007/s007010050491
DO - 10.1007/s007010050491
M3 - Article
C2 - 10550658
AN - SCOPUS:0032696663
SN - 0001-6268
VL - 141
SP - 1107
EP - 1113
JO - Acta Neurochirurgica
JF - Acta Neurochirurgica
IS - 10
ER -