Proteasomes in human spermatozoa

Cezary Wojcik, M. Benchaib, J. Lornage, J. C. Czyba, J. F. Guerin

Research output: Contribution to journalArticle

59 Citations (Scopus)

Abstract

In the present study we describe the localization of proteasomes in human spermatozoa by means of immunolabelling with different monoclonal and polyclonal antibodies detected by confocal microscopy. Western blotting confirmed the specificity of the antibodies and has shown that proteasomes are present in spermatozoa and in seminal fluid. In spermatozoa proteasomes are concentrated in the neck region where the centrioles are located. Some labelling was also detected at the periphery of the head, but no proteasomal antigens were detected in either the nucleus or associated with the flagellum. Proteasome inhibitors did not affect the motility of the spermatozoa, acrosome reaction nor zona binding. It is hypothesized that paternal proteasomes enter the oocyte during fertilization in tight association with the centrioles and may serve a special function during further development which can be associated with the function of a hypothetical proteolysis centre.

Original languageEnglish (US)
Pages (from-to)169-177
Number of pages9
JournalInternational Journal of Andrology
Volume23
Issue number3
DOIs
StatePublished - 2000
Externally publishedYes

Fingerprint

Proteasome Endopeptidase Complex
Spermatozoa
Centrioles
Acrosome Reaction
Proteasome Inhibitors
Antibody Specificity
Flagella
Herpes Zoster
Fertilization
Confocal Microscopy
Proteolysis
Oocytes
Neck
Western Blotting
Head
Monoclonal Antibodies
Antigens

Keywords

  • Proteasome
  • Proteasome activator
  • Proteolytic centre
  • Spermatozoa

ASJC Scopus subject areas

  • Endocrinology

Cite this

Wojcik, C., Benchaib, M., Lornage, J., Czyba, J. C., & Guerin, J. F. (2000). Proteasomes in human spermatozoa. International Journal of Andrology, 23(3), 169-177. https://doi.org/10.1046/j.1365-2605.2000.00223.x

Proteasomes in human spermatozoa. / Wojcik, Cezary; Benchaib, M.; Lornage, J.; Czyba, J. C.; Guerin, J. F.

In: International Journal of Andrology, Vol. 23, No. 3, 2000, p. 169-177.

Research output: Contribution to journalArticle

Wojcik, C, Benchaib, M, Lornage, J, Czyba, JC & Guerin, JF 2000, 'Proteasomes in human spermatozoa', International Journal of Andrology, vol. 23, no. 3, pp. 169-177. https://doi.org/10.1046/j.1365-2605.2000.00223.x
Wojcik, Cezary ; Benchaib, M. ; Lornage, J. ; Czyba, J. C. ; Guerin, J. F. / Proteasomes in human spermatozoa. In: International Journal of Andrology. 2000 ; Vol. 23, No. 3. pp. 169-177.
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