Proteasome activator (PA28) subunits, α, β and γ (Ki antigen) in NT2 neuronal precursor cells and HeLa S3 cells

Cezary Wojcik, Keiji Tanaka, Neidhard Paweletz, Ursula Naab, Sherwin Wilk

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

The catalytic activity of the 20S proteasome can be modulated by endogenous proteins. A proteasome activator protein termed PA28 or 11S regulator, composed of two homologous subunits (α and β) and a separate but related protein termed Ki antigen or PA28γ have been characterized. To explore the functional relationship of these proteins, NT2 clone D1 human neuronal precursor cells, as well as HeLa S3 cells were labeled by immunofluorescence and immunoelectron microscopy with three different antisera directed against peptides derived from their sequences. It was found that both PA28α and PA28β antisera label the cytoplasm and the nucleoli. In contrast, the PA28γ antiserum labels the nucleus but not the nucleoli while in the cytoplasm it labels two different classes of structures identified as microtubular-like extensions and inclusion bodies that are most likely autophagosomes. The latter do not contain proteasome δ subunit antigen. The microtubular-like structures colocalize with β-tubulin, are dispersed by nocodazole and are not affected by brefeldin A treatment. PA28α and PA28β are co-localized in the cell whereas PA28γ has a different distribution. PA28γ complexed with the proteasome may serve a function other than or in addition to activation and may also have a proteasome-independent function.

Original languageEnglish (US)
Pages (from-to)151-160
Number of pages10
JournalEuropean Journal of Cell Biology
Volume77
Issue number2
StatePublished - 1998
Externally publishedYes

Fingerprint

Proteasome Endopeptidase Complex
HeLa Cells
Immune Sera
Cytoplasm
Proteins
Nocodazole
Brefeldin A
Immunoelectron Microscopy
Inclusion Bodies
Tubulin
Fluorescence Microscopy
Clone Cells
Ki antigen
Antigens
Peptides

Keywords

  • HeLa cells
  • Ki antigen
  • NT2 cells
  • PA28
  • Proteasome
  • Proteasome activator

ASJC Scopus subject areas

  • Anatomy
  • Cell Biology

Cite this

Proteasome activator (PA28) subunits, α, β and γ (Ki antigen) in NT2 neuronal precursor cells and HeLa S3 cells. / Wojcik, Cezary; Tanaka, Keiji; Paweletz, Neidhard; Naab, Ursula; Wilk, Sherwin.

In: European Journal of Cell Biology, Vol. 77, No. 2, 1998, p. 151-160.

Research output: Contribution to journalArticle

Wojcik, Cezary ; Tanaka, Keiji ; Paweletz, Neidhard ; Naab, Ursula ; Wilk, Sherwin. / Proteasome activator (PA28) subunits, α, β and γ (Ki antigen) in NT2 neuronal precursor cells and HeLa S3 cells. In: European Journal of Cell Biology. 1998 ; Vol. 77, No. 2. pp. 151-160.
@article{605ca696a70b4bb9b3b2135e1789bf13,
title = "Proteasome activator (PA28) subunits, α, β and γ (Ki antigen) in NT2 neuronal precursor cells and HeLa S3 cells",
abstract = "The catalytic activity of the 20S proteasome can be modulated by endogenous proteins. A proteasome activator protein termed PA28 or 11S regulator, composed of two homologous subunits (α and β) and a separate but related protein termed Ki antigen or PA28γ have been characterized. To explore the functional relationship of these proteins, NT2 clone D1 human neuronal precursor cells, as well as HeLa S3 cells were labeled by immunofluorescence and immunoelectron microscopy with three different antisera directed against peptides derived from their sequences. It was found that both PA28α and PA28β antisera label the cytoplasm and the nucleoli. In contrast, the PA28γ antiserum labels the nucleus but not the nucleoli while in the cytoplasm it labels two different classes of structures identified as microtubular-like extensions and inclusion bodies that are most likely autophagosomes. The latter do not contain proteasome δ subunit antigen. The microtubular-like structures colocalize with β-tubulin, are dispersed by nocodazole and are not affected by brefeldin A treatment. PA28α and PA28β are co-localized in the cell whereas PA28γ has a different distribution. PA28γ complexed with the proteasome may serve a function other than or in addition to activation and may also have a proteasome-independent function.",
keywords = "HeLa cells, Ki antigen, NT2 cells, PA28, Proteasome, Proteasome activator",
author = "Cezary Wojcik and Keiji Tanaka and Neidhard Paweletz and Ursula Naab and Sherwin Wilk",
year = "1998",
language = "English (US)",
volume = "77",
pages = "151--160",
journal = "European Journal of Cell Biology",
issn = "0171-9335",
publisher = "Urban und Fischer Verlag GmbH und Co. KG",
number = "2",

}

TY - JOUR

T1 - Proteasome activator (PA28) subunits, α, β and γ (Ki antigen) in NT2 neuronal precursor cells and HeLa S3 cells

AU - Wojcik, Cezary

AU - Tanaka, Keiji

AU - Paweletz, Neidhard

AU - Naab, Ursula

AU - Wilk, Sherwin

PY - 1998

Y1 - 1998

N2 - The catalytic activity of the 20S proteasome can be modulated by endogenous proteins. A proteasome activator protein termed PA28 or 11S regulator, composed of two homologous subunits (α and β) and a separate but related protein termed Ki antigen or PA28γ have been characterized. To explore the functional relationship of these proteins, NT2 clone D1 human neuronal precursor cells, as well as HeLa S3 cells were labeled by immunofluorescence and immunoelectron microscopy with three different antisera directed against peptides derived from their sequences. It was found that both PA28α and PA28β antisera label the cytoplasm and the nucleoli. In contrast, the PA28γ antiserum labels the nucleus but not the nucleoli while in the cytoplasm it labels two different classes of structures identified as microtubular-like extensions and inclusion bodies that are most likely autophagosomes. The latter do not contain proteasome δ subunit antigen. The microtubular-like structures colocalize with β-tubulin, are dispersed by nocodazole and are not affected by brefeldin A treatment. PA28α and PA28β are co-localized in the cell whereas PA28γ has a different distribution. PA28γ complexed with the proteasome may serve a function other than or in addition to activation and may also have a proteasome-independent function.

AB - The catalytic activity of the 20S proteasome can be modulated by endogenous proteins. A proteasome activator protein termed PA28 or 11S regulator, composed of two homologous subunits (α and β) and a separate but related protein termed Ki antigen or PA28γ have been characterized. To explore the functional relationship of these proteins, NT2 clone D1 human neuronal precursor cells, as well as HeLa S3 cells were labeled by immunofluorescence and immunoelectron microscopy with three different antisera directed against peptides derived from their sequences. It was found that both PA28α and PA28β antisera label the cytoplasm and the nucleoli. In contrast, the PA28γ antiserum labels the nucleus but not the nucleoli while in the cytoplasm it labels two different classes of structures identified as microtubular-like extensions and inclusion bodies that are most likely autophagosomes. The latter do not contain proteasome δ subunit antigen. The microtubular-like structures colocalize with β-tubulin, are dispersed by nocodazole and are not affected by brefeldin A treatment. PA28α and PA28β are co-localized in the cell whereas PA28γ has a different distribution. PA28γ complexed with the proteasome may serve a function other than or in addition to activation and may also have a proteasome-independent function.

KW - HeLa cells

KW - Ki antigen

KW - NT2 cells

KW - PA28

KW - Proteasome

KW - Proteasome activator

UR - http://www.scopus.com/inward/record.url?scp=0031770396&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031770396&partnerID=8YFLogxK

M3 - Article

C2 - 9840465

AN - SCOPUS:0031770396

VL - 77

SP - 151

EP - 160

JO - European Journal of Cell Biology

JF - European Journal of Cell Biology

SN - 0171-9335

IS - 2

ER -