Prostatic tissue testosterone and dihydrotestosterone in African-American and white men

Leonard S. Marks, David Hess, Frederick J. Dorey, Maria L. Macairan

    Research output: Contribution to journalArticle

    15 Citations (Scopus)

    Abstract

    Objectives: To compare tissue androgen levels in the prostate gland of African-American and white men, looking for a possible explanation of the increased incidence of cancer in the former. Methods: The subjects were 25 African-American and 36 white men, undergoing prostate biopsy consecutively, in whom cancer was absent. Biopsy cores (18 gauge) from the peripheral zone were homogenized, subjected to ether extraction, and separation by chromatography. Tissue testosterone and dihydrotestosterone (DHT) levels were determined by radioimmunoassay. Results: The groups were matched for mean age (67.6 ± 9.6 years), prostate volume (37.9 ± 21.0 cm3), body mass index (28.2 ± 4.2 kg/m2), and serum prostate-specific antigen (2.8 to 3.4 ng/mL) and testosterone (330 ± 114 ng/dL) levels (P = NS for all measures). No significant difference in tissue testosterone (median 0.8 ng/g) or DHT (median 4.6 ng/g) was found between groups (P = NS). Furthermore, the tissue DHT/testosterone ratio (∼5) was not significantly different between the two groups (P = NS). Conclusions: Prostatic tissue levels of testosterone and DHT were similar in African-American and white men; thus, the present data do not support a hypothesis of increased androgenic activity in African-American men. Because the ratio of DHT/testosterone in prostatic tissue was similar in the two groups, the possibility of increased 5-alpha-reductase activity in African-American men did not seem likely. Using needle biopsy specimens, both absolute values and the ratio of the androgens in prostatic tissue were similar to those found in previous studies using surgically excised glands. Thus, quick-frozen biopsy cores appear to be a valuable tissue source for evaluating the androgen status within the prostate.

    Original languageEnglish (US)
    Pages (from-to)337-341
    Number of pages5
    JournalUrology
    Volume68
    Issue number2
    DOIs
    StatePublished - Aug 2006

    Fingerprint

    Dihydrotestosterone
    African Americans
    Testosterone
    Prostate
    Androgens
    Biopsy
    Cholestenone 5 alpha-Reductase
    Needle Biopsy
    Prostate-Specific Antigen
    Ether
    Radioimmunoassay
    Chromatography
    Neoplasms
    Body Mass Index
    Research Design
    Incidence
    Serum

    ASJC Scopus subject areas

    • Urology

    Cite this

    Prostatic tissue testosterone and dihydrotestosterone in African-American and white men. / Marks, Leonard S.; Hess, David; Dorey, Frederick J.; Macairan, Maria L.

    In: Urology, Vol. 68, No. 2, 08.2006, p. 337-341.

    Research output: Contribution to journalArticle

    Marks, Leonard S. ; Hess, David ; Dorey, Frederick J. ; Macairan, Maria L. / Prostatic tissue testosterone and dihydrotestosterone in African-American and white men. In: Urology. 2006 ; Vol. 68, No. 2. pp. 337-341.
    @article{a65656a17e0a463c840e7af123a02bbd,
    title = "Prostatic tissue testosterone and dihydrotestosterone in African-American and white men",
    abstract = "Objectives: To compare tissue androgen levels in the prostate gland of African-American and white men, looking for a possible explanation of the increased incidence of cancer in the former. Methods: The subjects were 25 African-American and 36 white men, undergoing prostate biopsy consecutively, in whom cancer was absent. Biopsy cores (18 gauge) from the peripheral zone were homogenized, subjected to ether extraction, and separation by chromatography. Tissue testosterone and dihydrotestosterone (DHT) levels were determined by radioimmunoassay. Results: The groups were matched for mean age (67.6 ± 9.6 years), prostate volume (37.9 ± 21.0 cm3), body mass index (28.2 ± 4.2 kg/m2), and serum prostate-specific antigen (2.8 to 3.4 ng/mL) and testosterone (330 ± 114 ng/dL) levels (P = NS for all measures). No significant difference in tissue testosterone (median 0.8 ng/g) or DHT (median 4.6 ng/g) was found between groups (P = NS). Furthermore, the tissue DHT/testosterone ratio (∼5) was not significantly different between the two groups (P = NS). Conclusions: Prostatic tissue levels of testosterone and DHT were similar in African-American and white men; thus, the present data do not support a hypothesis of increased androgenic activity in African-American men. Because the ratio of DHT/testosterone in prostatic tissue was similar in the two groups, the possibility of increased 5-alpha-reductase activity in African-American men did not seem likely. Using needle biopsy specimens, both absolute values and the ratio of the androgens in prostatic tissue were similar to those found in previous studies using surgically excised glands. Thus, quick-frozen biopsy cores appear to be a valuable tissue source for evaluating the androgen status within the prostate.",
    author = "Marks, {Leonard S.} and David Hess and Dorey, {Frederick J.} and Macairan, {Maria L.}",
    year = "2006",
    month = "8",
    doi = "10.1016/j.urology.2006.03.013",
    language = "English (US)",
    volume = "68",
    pages = "337--341",
    journal = "Urology",
    issn = "0090-4295",
    publisher = "Elsevier Inc.",
    number = "2",

    }

    TY - JOUR

    T1 - Prostatic tissue testosterone and dihydrotestosterone in African-American and white men

    AU - Marks, Leonard S.

    AU - Hess, David

    AU - Dorey, Frederick J.

    AU - Macairan, Maria L.

    PY - 2006/8

    Y1 - 2006/8

    N2 - Objectives: To compare tissue androgen levels in the prostate gland of African-American and white men, looking for a possible explanation of the increased incidence of cancer in the former. Methods: The subjects were 25 African-American and 36 white men, undergoing prostate biopsy consecutively, in whom cancer was absent. Biopsy cores (18 gauge) from the peripheral zone were homogenized, subjected to ether extraction, and separation by chromatography. Tissue testosterone and dihydrotestosterone (DHT) levels were determined by radioimmunoassay. Results: The groups were matched for mean age (67.6 ± 9.6 years), prostate volume (37.9 ± 21.0 cm3), body mass index (28.2 ± 4.2 kg/m2), and serum prostate-specific antigen (2.8 to 3.4 ng/mL) and testosterone (330 ± 114 ng/dL) levels (P = NS for all measures). No significant difference in tissue testosterone (median 0.8 ng/g) or DHT (median 4.6 ng/g) was found between groups (P = NS). Furthermore, the tissue DHT/testosterone ratio (∼5) was not significantly different between the two groups (P = NS). Conclusions: Prostatic tissue levels of testosterone and DHT were similar in African-American and white men; thus, the present data do not support a hypothesis of increased androgenic activity in African-American men. Because the ratio of DHT/testosterone in prostatic tissue was similar in the two groups, the possibility of increased 5-alpha-reductase activity in African-American men did not seem likely. Using needle biopsy specimens, both absolute values and the ratio of the androgens in prostatic tissue were similar to those found in previous studies using surgically excised glands. Thus, quick-frozen biopsy cores appear to be a valuable tissue source for evaluating the androgen status within the prostate.

    AB - Objectives: To compare tissue androgen levels in the prostate gland of African-American and white men, looking for a possible explanation of the increased incidence of cancer in the former. Methods: The subjects were 25 African-American and 36 white men, undergoing prostate biopsy consecutively, in whom cancer was absent. Biopsy cores (18 gauge) from the peripheral zone were homogenized, subjected to ether extraction, and separation by chromatography. Tissue testosterone and dihydrotestosterone (DHT) levels were determined by radioimmunoassay. Results: The groups were matched for mean age (67.6 ± 9.6 years), prostate volume (37.9 ± 21.0 cm3), body mass index (28.2 ± 4.2 kg/m2), and serum prostate-specific antigen (2.8 to 3.4 ng/mL) and testosterone (330 ± 114 ng/dL) levels (P = NS for all measures). No significant difference in tissue testosterone (median 0.8 ng/g) or DHT (median 4.6 ng/g) was found between groups (P = NS). Furthermore, the tissue DHT/testosterone ratio (∼5) was not significantly different between the two groups (P = NS). Conclusions: Prostatic tissue levels of testosterone and DHT were similar in African-American and white men; thus, the present data do not support a hypothesis of increased androgenic activity in African-American men. Because the ratio of DHT/testosterone in prostatic tissue was similar in the two groups, the possibility of increased 5-alpha-reductase activity in African-American men did not seem likely. Using needle biopsy specimens, both absolute values and the ratio of the androgens in prostatic tissue were similar to those found in previous studies using surgically excised glands. Thus, quick-frozen biopsy cores appear to be a valuable tissue source for evaluating the androgen status within the prostate.

    UR - http://www.scopus.com/inward/record.url?scp=33746849355&partnerID=8YFLogxK

    UR - http://www.scopus.com/inward/citedby.url?scp=33746849355&partnerID=8YFLogxK

    U2 - 10.1016/j.urology.2006.03.013

    DO - 10.1016/j.urology.2006.03.013

    M3 - Article

    VL - 68

    SP - 337

    EP - 341

    JO - Urology

    JF - Urology

    SN - 0090-4295

    IS - 2

    ER -