Prostaglandin receptors in lower segment myometrium during gestation and labor

Julia Brodt-Eppley, Leslie Myatt

Research output: Contribution to journalArticle

135 Citations (Scopus)

Abstract

Objective: To determine if expression of relaxatory prostaglandin (PG) E2 receptors (EP2 isoform) and contractile PG F receptors (FP isoform) changes during gestation and with labor in pregnant human myometrium. Methods: Lower segment myometrium was removed at cesarean from four groups of parturients (preterm [28-36 weeks] or term [37-41 weeks], either in or not in labor, ten per group). Myometrial RNA was isolated and used for reverse transcription-polymerase chain reaction (PCR) analysis with specific primer sets for human smooth muscle protein calponin and PGEP2 or FP receptor isoform mRNA. Polymerase chain reaction products were electrophoresed on gels and visualized, band intensity was measured, and EP2 and FP receptor expression was normalized to calponin. Results: Reverse transcription-PCR yielded products of expected sizes, and restriction enzyme cleavage confirmed identities. Prostaglandin EP2 receptor isoform mRNA expression (relative to calponin mRNA) was significantly greater in the preterm, no labor group (1.28 ± 0.22, mean ± standard error of the mean [SE]) compared with the term, no labor group (0.71 ± 0.09) (P <.05 Student-Neuman-Kuels) and declined significantly with gestational age in patients not in labor (R = -.446, P = .001). Prostaglandin FP receptor isoform mRNA expression was significantly less in the term, no labor group (0.61 ± 0.06) compared with all others (P <.02 Student-Neuman-Kuels). Prostaglandin FP receptor expression declined significantly with gestational age in patients not in labor (R = -.646, P = .012) and increased significantly with labor at term. Conclusion: Changes in prostaglandin EP2 receptor isoform expression are consistent with influence on maintenance of quiescence. Labor at term is associated with a significant increase in FP receptor expression, consistent with influence on contraction. The balance between the two receptor isoforms might mediate myometrial contractility. (Obstet Gynecol 1999;93:89-93.

Original languageEnglish (US)
Pages (from-to)89-93
Number of pages5
JournalObstetrics and Gynecology
Volume93
Issue number1
DOIs
StatePublished - Jan 1999
Externally publishedYes

Fingerprint

Prostaglandin Receptors
Myometrium
RNA Isoforms
Pregnancy
Protein Isoforms
Polymerase Chain Reaction
Gestational Age
Reverse Transcription
Students
Premature Obstetric Labor
Prostaglandins F
Dinoprostone
Gels
Maintenance
prostaglandin F2alpha receptor
Parturition
RNA
Messenger RNA
Enzymes
calponin

ASJC Scopus subject areas

  • Obstetrics and Gynecology

Cite this

Prostaglandin receptors in lower segment myometrium during gestation and labor. / Brodt-Eppley, Julia; Myatt, Leslie.

In: Obstetrics and Gynecology, Vol. 93, No. 1, 01.1999, p. 89-93.

Research output: Contribution to journalArticle

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abstract = "Objective: To determine if expression of relaxatory prostaglandin (PG) E2 receptors (EP2 isoform) and contractile PG F receptors (FP isoform) changes during gestation and with labor in pregnant human myometrium. Methods: Lower segment myometrium was removed at cesarean from four groups of parturients (preterm [28-36 weeks] or term [37-41 weeks], either in or not in labor, ten per group). Myometrial RNA was isolated and used for reverse transcription-polymerase chain reaction (PCR) analysis with specific primer sets for human smooth muscle protein calponin and PGEP2 or FP receptor isoform mRNA. Polymerase chain reaction products were electrophoresed on gels and visualized, band intensity was measured, and EP2 and FP receptor expression was normalized to calponin. Results: Reverse transcription-PCR yielded products of expected sizes, and restriction enzyme cleavage confirmed identities. Prostaglandin EP2 receptor isoform mRNA expression (relative to calponin mRNA) was significantly greater in the preterm, no labor group (1.28 ± 0.22, mean ± standard error of the mean [SE]) compared with the term, no labor group (0.71 ± 0.09) (P <.05 Student-Neuman-Kuels) and declined significantly with gestational age in patients not in labor (R = -.446, P = .001). Prostaglandin FP receptor isoform mRNA expression was significantly less in the term, no labor group (0.61 ± 0.06) compared with all others (P <.02 Student-Neuman-Kuels). Prostaglandin FP receptor expression declined significantly with gestational age in patients not in labor (R = -.646, P = .012) and increased significantly with labor at term. Conclusion: Changes in prostaglandin EP2 receptor isoform expression are consistent with influence on maintenance of quiescence. Labor at term is associated with a significant increase in FP receptor expression, consistent with influence on contraction. The balance between the two receptor isoforms might mediate myometrial contractility. (Obstet Gynecol 1999;93:89-93.",
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