Prostaglandin F2-alpha receptor regulation in human uterine myocytes

Z. Liang, S. R. Sooranna, N. Engineer, M. Tattersall, S. Khanjani, P. R. Bennett, Leslie Myatt, Mark R. Johnson

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Investigations of the modulation of prostaglandin F receptor (FP) expression in primary cultures of human uterine myocytes showed that FP mRNA expression was reduced by progesterone, unaltered by cAMP (8-bromo cAMP or forskolin), but increased by the PKA antagonist H89. Interleukin (IL)-1β, tumour necrosis factor-alpha and oxytocin increased FP mRNA expression and IL-6 and prostaglandin E2 reduced FP mRNA expression. The changes in FP protein levels were similar to the mRNA responses. We found that the IL-1β-induced increase in FP expression was mediated at least in part via protein kinase C (PKC), but was independent of mitogen-activated protein kinase, phospholipase C and PI3 kinase. Since IL-1β activates NFκB, AP-1 and C/EBP, we over-expressed these transcription factors alone and in combination and found that only NFκB alone increased FP mRNA expression. Finally, we found that the IL-1β-induced increase in FP expression was unaffected by progesterone and/or cAMP, but was accentuated by H89. These data suggest that the pregnancy-induced down-regulation in myometrial FP expression is mediated by progesterone and cAMP and that the increase with labour is induced by inflammatory cytokine activation of PKC and NFκB.

Original languageEnglish (US)
Pages (from-to)215-223
Number of pages9
JournalMolecular Human Reproduction
Volume14
Issue number4
DOIs
StatePublished - Apr 2008
Externally publishedYes

Fingerprint

Dinoprost
Muscle Cells
Interleukin-1
Messenger RNA
Progesterone
Protein Kinase C
Induced Labor
8-Bromo Cyclic Adenosine Monophosphate
Transcription Factor AP-1
Type C Phospholipases
Colforsin
Oxytocin
Mitogen-Activated Protein Kinases
Phosphatidylinositol 3-Kinases
Dinoprostone
Interleukin-6
Transcription Factors
Down-Regulation
Tumor Necrosis Factor-alpha
prostaglandin F2alpha receptor

Keywords

  • Inflammatory cytokines
  • Labour
  • NFκB
  • Progesterone
  • Prostaglandin F receptor

ASJC Scopus subject areas

  • Obstetrics and Gynecology
  • Genetics
  • Developmental Biology
  • Embryology
  • Cell Biology

Cite this

Liang, Z., Sooranna, S. R., Engineer, N., Tattersall, M., Khanjani, S., Bennett, P. R., ... Johnson, M. R. (2008). Prostaglandin F2-alpha receptor regulation in human uterine myocytes. Molecular Human Reproduction, 14(4), 215-223. https://doi.org/10.1093/molehr/gan008

Prostaglandin F2-alpha receptor regulation in human uterine myocytes. / Liang, Z.; Sooranna, S. R.; Engineer, N.; Tattersall, M.; Khanjani, S.; Bennett, P. R.; Myatt, Leslie; Johnson, Mark R.

In: Molecular Human Reproduction, Vol. 14, No. 4, 04.2008, p. 215-223.

Research output: Contribution to journalArticle

Liang, Z, Sooranna, SR, Engineer, N, Tattersall, M, Khanjani, S, Bennett, PR, Myatt, L & Johnson, MR 2008, 'Prostaglandin F2-alpha receptor regulation in human uterine myocytes', Molecular Human Reproduction, vol. 14, no. 4, pp. 215-223. https://doi.org/10.1093/molehr/gan008
Liang Z, Sooranna SR, Engineer N, Tattersall M, Khanjani S, Bennett PR et al. Prostaglandin F2-alpha receptor regulation in human uterine myocytes. Molecular Human Reproduction. 2008 Apr;14(4):215-223. https://doi.org/10.1093/molehr/gan008
Liang, Z. ; Sooranna, S. R. ; Engineer, N. ; Tattersall, M. ; Khanjani, S. ; Bennett, P. R. ; Myatt, Leslie ; Johnson, Mark R. / Prostaglandin F2-alpha receptor regulation in human uterine myocytes. In: Molecular Human Reproduction. 2008 ; Vol. 14, No. 4. pp. 215-223.
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