Propulsive forces in keratocyte migration

Catherine Galbraith, M. P. Sheetz

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

To move forward, migrating cells pull rearward against extracellular matrix and exert traction forces. To elucidate the mechanism of traction force generation in keratocytes, we have measured the forces generated against ECM contacts on both the ventral and the dorsal surfaces of the lamella. We used a micromachined substrate to measure forces on the ventral surface and an optical gradient trap to measure forces on the dorsal surface. Both methods determined comparable forces per unit area that were rearward in the front of the lamella, increasing toward the perinuclear region, and eventually changing direction. The rearward traction forces generated throughout the lamella suggest that myosin condensation at the cell body boundary pulls the lamella actin network rearward and pushes the cell body forward.

Original languageEnglish (US)
Title of host publicationAnnual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings
PublisherIEEE
Pages100
Number of pages1
Volume1
ISBN (Print)0780356756
StatePublished - 1999
Externally publishedYes
EventProceedings of the 1999 IEEE Engineering in Medicine and Biology 21st Annual Conference and the 1999 Fall Meeting of the Biomedical Engineering Society (1st Joint BMES / EMBS) - Atlanta, GA, USA
Duration: Oct 13 1999Oct 16 1999

Other

OtherProceedings of the 1999 IEEE Engineering in Medicine and Biology 21st Annual Conference and the 1999 Fall Meeting of the Biomedical Engineering Society (1st Joint BMES / EMBS)
CityAtlanta, GA, USA
Period10/13/9910/16/99

Fingerprint

Cells
Military electronic countermeasures
Myosins
Actins
Condensation
Substrates
Direction compound

ASJC Scopus subject areas

  • Bioengineering

Cite this

Galbraith, C., & Sheetz, M. P. (1999). Propulsive forces in keratocyte migration. In Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings (Vol. 1, pp. 100). IEEE.

Propulsive forces in keratocyte migration. / Galbraith, Catherine; Sheetz, M. P.

Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings. Vol. 1 IEEE, 1999. p. 100.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Galbraith, C & Sheetz, MP 1999, Propulsive forces in keratocyte migration. in Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings. vol. 1, IEEE, pp. 100, Proceedings of the 1999 IEEE Engineering in Medicine and Biology 21st Annual Conference and the 1999 Fall Meeting of the Biomedical Engineering Society (1st Joint BMES / EMBS), Atlanta, GA, USA, 10/13/99.
Galbraith C, Sheetz MP. Propulsive forces in keratocyte migration. In Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings. Vol. 1. IEEE. 1999. p. 100
Galbraith, Catherine ; Sheetz, M. P. / Propulsive forces in keratocyte migration. Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings. Vol. 1 IEEE, 1999. pp. 100
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