Propulsive forces in keratocyte migration

C. G. Galbraith, M. P. Sheetz

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

To move forward, migrating cells pull rearward against extracellular matrix and exert traction forces. To elucidate the mechanism of traction force generation in keratocytes, we have measured the forces generated against ECM contacts on both the ventral and the dorsal surfaces of the lamella. We used a micromachined substrate to measure forces on the ventral surface and an optical gradient trap to measure forces on the dorsal surface. Both methods determined comparable forces per unit area that were rearward in the front of the lamella, increasing toward the perinuclear region, and eventually changing direction. The rearward traction forces generated throughout the lamella suggest that myosin condensation at the cell body boundary pulls the lamella actin network rearward and pushes the cell body forward.

Original languageEnglish (US)
Title of host publicationAnnual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings
PublisherIEEE
Number of pages1
ISBN (Print)0780356756
StatePublished - Dec 1 1999
EventProceedings of the 1999 IEEE Engineering in Medicine and Biology 21st Annual Conference and the 1999 Fall Meeting of the Biomedical Engineering Society (1st Joint BMES / EMBS) - Atlanta, GA, USA
Duration: Oct 13 1999Oct 16 1999

Publication series

NameAnnual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings
Volume1
ISSN (Print)0589-1019

Other

OtherProceedings of the 1999 IEEE Engineering in Medicine and Biology 21st Annual Conference and the 1999 Fall Meeting of the Biomedical Engineering Society (1st Joint BMES / EMBS)
CityAtlanta, GA, USA
Period10/13/9910/16/99

ASJC Scopus subject areas

  • Signal Processing
  • Biomedical Engineering
  • Computer Vision and Pattern Recognition
  • Health Informatics

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