Promotion of fibronectin independent invasion by C5a peptidase into epithelial cells in group A Streptococcus

Sai Sudha Purushothaman, Haesun Park, P. Patrick Cleary

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Background & objectives: Group A Streptococcus, causative agent of several clinical manifestations codes for multiple protein invasins which help the bacterium to enter non-phagocytic cells. C5a peptidase (SCPA) is a surface protein conserved among different serotypes of M1 strain. The present study was taken up to study SCPA promoted fibronectin independent entry of GAS into epithelial cells. Methods: An isogenic 90226 emm1ΔAB (M1-) mutant was constructed with thermosensitive pGhost vector. This isogenic M1- mutant expressed SCPA on the surface as determined by Western blotting and immunofluorescence. Results: On preincubation with anti-SCPA serum, the isogenic M1- strain exhibited 54 per cent decreased invasion as compared to the bacteria incubated with control serum. Also, purified recombinant SCPA proteins blocked internalization of M1- streptococci into HEp-2 cells. The M1- strain invaded at the same efficiency in the presence or absence of fibronectin. Interpretation & conclusion: These results suggested that SCPA acted as a potential invasin of group A streptococcus and promoted invasion independent of fibronectin.

Original languageEnglish (US)
Pages (from-to)44-47
Number of pages4
JournalIndian Journal of Medical Research, Supplement
Volume119
Issue numberMAY
StatePublished - 2004
Externally publishedYes

Fingerprint

Streptococcus
Fibronectins
Epithelial Cells
Bacteria
Recombinant proteins
Serum
Recombinant Proteins
Fluorescent Antibody Technique
small cardioactive peptide A
C5a peptidase
Membrane Proteins
Western Blotting
Proteins

Keywords

  • C5a peptidase
  • Fibronectin
  • Group A streptococcus
  • Invasion
  • M1 strain

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Promotion of fibronectin independent invasion by C5a peptidase into epithelial cells in group A Streptococcus. / Purushothaman, Sai Sudha; Park, Haesun; Cleary, P. Patrick.

In: Indian Journal of Medical Research, Supplement, Vol. 119, No. MAY, 2004, p. 44-47.

Research output: Contribution to journalArticle

@article{8ecfd9952acc463fa658edf30e95cd7d,
title = "Promotion of fibronectin independent invasion by C5a peptidase into epithelial cells in group A Streptococcus",
abstract = "Background & objectives: Group A Streptococcus, causative agent of several clinical manifestations codes for multiple protein invasins which help the bacterium to enter non-phagocytic cells. C5a peptidase (SCPA) is a surface protein conserved among different serotypes of M1 strain. The present study was taken up to study SCPA promoted fibronectin independent entry of GAS into epithelial cells. Methods: An isogenic 90226 emm1ΔAB (M1-) mutant was constructed with thermosensitive pGhost vector. This isogenic M1- mutant expressed SCPA on the surface as determined by Western blotting and immunofluorescence. Results: On preincubation with anti-SCPA serum, the isogenic M1- strain exhibited 54 per cent decreased invasion as compared to the bacteria incubated with control serum. Also, purified recombinant SCPA proteins blocked internalization of M1- streptococci into HEp-2 cells. The M1- strain invaded at the same efficiency in the presence or absence of fibronectin. Interpretation & conclusion: These results suggested that SCPA acted as a potential invasin of group A streptococcus and promoted invasion independent of fibronectin.",
keywords = "C5a peptidase, Fibronectin, Group A streptococcus, Invasion, M1 strain",
author = "Purushothaman, {Sai Sudha} and Haesun Park and Cleary, {P. Patrick}",
year = "2004",
language = "English (US)",
volume = "119",
pages = "44--47",
journal = "Indian Journal of Medical Research, Supplement",
issn = "0367-9012",
publisher = "Indian Council of Medical Research",
number = "MAY",

}

TY - JOUR

T1 - Promotion of fibronectin independent invasion by C5a peptidase into epithelial cells in group A Streptococcus

AU - Purushothaman, Sai Sudha

AU - Park, Haesun

AU - Cleary, P. Patrick

PY - 2004

Y1 - 2004

N2 - Background & objectives: Group A Streptococcus, causative agent of several clinical manifestations codes for multiple protein invasins which help the bacterium to enter non-phagocytic cells. C5a peptidase (SCPA) is a surface protein conserved among different serotypes of M1 strain. The present study was taken up to study SCPA promoted fibronectin independent entry of GAS into epithelial cells. Methods: An isogenic 90226 emm1ΔAB (M1-) mutant was constructed with thermosensitive pGhost vector. This isogenic M1- mutant expressed SCPA on the surface as determined by Western blotting and immunofluorescence. Results: On preincubation with anti-SCPA serum, the isogenic M1- strain exhibited 54 per cent decreased invasion as compared to the bacteria incubated with control serum. Also, purified recombinant SCPA proteins blocked internalization of M1- streptococci into HEp-2 cells. The M1- strain invaded at the same efficiency in the presence or absence of fibronectin. Interpretation & conclusion: These results suggested that SCPA acted as a potential invasin of group A streptococcus and promoted invasion independent of fibronectin.

AB - Background & objectives: Group A Streptococcus, causative agent of several clinical manifestations codes for multiple protein invasins which help the bacterium to enter non-phagocytic cells. C5a peptidase (SCPA) is a surface protein conserved among different serotypes of M1 strain. The present study was taken up to study SCPA promoted fibronectin independent entry of GAS into epithelial cells. Methods: An isogenic 90226 emm1ΔAB (M1-) mutant was constructed with thermosensitive pGhost vector. This isogenic M1- mutant expressed SCPA on the surface as determined by Western blotting and immunofluorescence. Results: On preincubation with anti-SCPA serum, the isogenic M1- strain exhibited 54 per cent decreased invasion as compared to the bacteria incubated with control serum. Also, purified recombinant SCPA proteins blocked internalization of M1- streptococci into HEp-2 cells. The M1- strain invaded at the same efficiency in the presence or absence of fibronectin. Interpretation & conclusion: These results suggested that SCPA acted as a potential invasin of group A streptococcus and promoted invasion independent of fibronectin.

KW - C5a peptidase

KW - Fibronectin

KW - Group A streptococcus

KW - Invasion

KW - M1 strain

UR - http://www.scopus.com/inward/record.url?scp=3442881063&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=3442881063&partnerID=8YFLogxK

M3 - Article

VL - 119

SP - 44

EP - 47

JO - Indian Journal of Medical Research, Supplement

JF - Indian Journal of Medical Research, Supplement

SN - 0367-9012

IS - MAY

ER -