Promotion of experimental thrombus formation by the procoagulant activity of breast cancer cells

M. A. Berny-Lang, Joseph Aslan, G. W. Tormoen, I. A. Patel, P. E. Bock, Andras Gruber, Owen McCarty

Research output: Contribution to journalArticle

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Abstract

The routine observation of tumor emboli in the peripheral blood of patients with carcinomas raises questions about the clinical relevance of these circulating tumor cells. Thrombosis is a common clinical manifestation of cancer, and circulating tumor cells may play a pathogenetic role in this process. The presence of coagulation-associated molecules on cancer cells has been described, but the mechanisms by which circulating tumor cells augment or alter coagulation remains unclear. In this study we utilized suspensions of a metastatic adenocarcinoma cell line, MDA-MB-231, and a non-metastatic breast epithelial cell line, MCF-10A, as models of circulating tumor cells to determine the thromobogenic activity of these blood-foreign cells. In human plasma, both metastatic MDA-MB-231 cells and non-metastatic MCF-10A cells significantly enhanced clotting kinetics. The effect of MDA-MB-231 and MCF-10A cells on clotting times was cell number-dependent and inhibited by a neutralizing antibody to tissue factor (TF) as well as inhibitors of activated factor X and thrombin. Using fluorescence microscopy, we found that both MDA-MB-231 and MCF-10A cells supported the binding of fluorescently labeled thrombin. Furthermore, in a model of thrombus formation under pressure-driven flow, MDA-MB-231 and MCF-10A cells significantly decreased the time to occlusion. Our findings indicate that the presence of breast epithelial cells in blood can stimulate coagulation in a TF-dependent manner, suggesting that tumor cells that enter the irculation may promote the formation of occlusive thrombi under shear flow conditions.

Original languageEnglish (US)
Article number015014
JournalPhysical Biology
Volume8
Issue number1
DOIs
StatePublished - 2011

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Thrombosis
Circulating Neoplastic Cells
Breast Neoplasms
Thromboplastin
Thrombin
Neoplasms
Breast
Epithelial Cells
Cell Line
Factor Xa
Neutralizing Antibodies
Embolism
Fluorescence Microscopy
Blood Cells
Suspensions
Adenocarcinoma
Cell Count
Observation
Carcinoma
Pressure

ASJC Scopus subject areas

  • Biophysics
  • Molecular Biology
  • Cell Biology
  • Structural Biology

Cite this

Promotion of experimental thrombus formation by the procoagulant activity of breast cancer cells. / Berny-Lang, M. A.; Aslan, Joseph; Tormoen, G. W.; Patel, I. A.; Bock, P. E.; Gruber, Andras; McCarty, Owen.

In: Physical Biology, Vol. 8, No. 1, 015014, 2011.

Research output: Contribution to journalArticle

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