Prolonged survival of adult rat pancreatic islets transfected with E1A- 12s adenovirus

Michael Rutten, Linda R. Lester, Margaret P. Quinlan, Charles K. Meshul, Clifford Deveney, John M. Rabkin

Research output: Contribution to journalArticle

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Abstract

It has been reported that various mutants of the El A-adenovirus can activate quiescent differentiated cells to start proliferating. The aim of this study was to determine whether transfection with E1A-12S could extend the life span and functionality of pancreatic islets in culture. Rat pancreatic islets were isolated and transfected with retrovirus containing the adenovirus E1A- 12S, E1A-13S, or control vectors. Transfection with the retroviral E1A-13S mutant produced extensive islet necrosis compared with nontransfected islets. Islets transfected with the control E1A mutant Ad5- d1312 vector (containing no E1A-12S or E1A-13S segments) were similar to non- transfected islets in their characteristics. We found that the E1A-12S transfected islets maintained greater viability, insulin granule structure, and glucose-induced insulin responsiveness over a 6-week period compared with mock or control islets. At 6 weeks of culture, the E1A-12S transfected islets also had fewer apoptotic cells compared with nontransfected islets. These data suggest that adenovirus E1A-12S can extend the functional life span of cultured rat pancreatic islets.

Original languageEnglish (US)
Pages (from-to)183-192
Number of pages10
JournalPancreas
Volume19
Issue number2
StatePublished - Aug 1999

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Islets of Langerhans
Adenoviridae
Transfection
Insulin
Retroviridae
Necrosis
Glucose

Keywords

  • E1A-12S
  • Glucose
  • Insulin
  • Islets
  • Pancreas
  • Rat
  • Retrovirus
  • Transfection

ASJC Scopus subject areas

  • Gastroenterology
  • Endocrinology

Cite this

Rutten, M., Lester, L. R., Quinlan, M. P., Meshul, C. K., Deveney, C., & Rabkin, J. M. (1999). Prolonged survival of adult rat pancreatic islets transfected with E1A- 12s adenovirus. Pancreas, 19(2), 183-192.

Prolonged survival of adult rat pancreatic islets transfected with E1A- 12s adenovirus. / Rutten, Michael; Lester, Linda R.; Quinlan, Margaret P.; Meshul, Charles K.; Deveney, Clifford; Rabkin, John M.

In: Pancreas, Vol. 19, No. 2, 08.1999, p. 183-192.

Research output: Contribution to journalArticle

Rutten, M, Lester, LR, Quinlan, MP, Meshul, CK, Deveney, C & Rabkin, JM 1999, 'Prolonged survival of adult rat pancreatic islets transfected with E1A- 12s adenovirus', Pancreas, vol. 19, no. 2, pp. 183-192.
Rutten M, Lester LR, Quinlan MP, Meshul CK, Deveney C, Rabkin JM. Prolonged survival of adult rat pancreatic islets transfected with E1A- 12s adenovirus. Pancreas. 1999 Aug;19(2):183-192.
Rutten, Michael ; Lester, Linda R. ; Quinlan, Margaret P. ; Meshul, Charles K. ; Deveney, Clifford ; Rabkin, John M. / Prolonged survival of adult rat pancreatic islets transfected with E1A- 12s adenovirus. In: Pancreas. 1999 ; Vol. 19, No. 2. pp. 183-192.
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