Prolonged survival of adult rat pancreatic islets transfected with E1A- 12s adenovirus

Michael J. Rutten, Linda R. Lester, Margaret P. Quinlan, Charles K. Meshul, Clifford W. Deveney, John M. Rabkin

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

It has been reported that various mutants of the El A-adenovirus can activate quiescent differentiated cells to start proliferating. The aim of this study was to determine whether transfection with E1A-12S could extend the life span and functionality of pancreatic islets in culture. Rat pancreatic islets were isolated and transfected with retrovirus containing the adenovirus E1A- 12S, E1A-13S, or control vectors. Transfection with the retroviral E1A-13S mutant produced extensive islet necrosis compared with nontransfected islets. Islets transfected with the control E1A mutant Ad5- d1312 vector (containing no E1A-12S or E1A-13S segments) were similar to non- transfected islets in their characteristics. We found that the E1A-12S transfected islets maintained greater viability, insulin granule structure, and glucose-induced insulin responsiveness over a 6-week period compared with mock or control islets. At 6 weeks of culture, the E1A-12S transfected islets also had fewer apoptotic cells compared with nontransfected islets. These data suggest that adenovirus E1A-12S can extend the functional life span of cultured rat pancreatic islets.

Original languageEnglish (US)
Pages (from-to)183-192
Number of pages10
JournalPancreas
Volume19
Issue number2
DOIs
StatePublished - Aug 1999

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Keywords

  • E1A-12S
  • Glucose
  • Insulin
  • Islets
  • Pancreas
  • Rat
  • Retrovirus
  • Transfection

ASJC Scopus subject areas

  • Internal Medicine
  • Endocrinology, Diabetes and Metabolism
  • Hepatology
  • Endocrinology

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