Abstract
It has been reported that various mutants of the El A-adenovirus can activate quiescent differentiated cells to start proliferating. The aim of this study was to determine whether transfection with E1A-12S could extend the life span and functionality of pancreatic islets in culture. Rat pancreatic islets were isolated and transfected with retrovirus containing the adenovirus E1A- 12S, E1A-13S, or control vectors. Transfection with the retroviral E1A-13S mutant produced extensive islet necrosis compared with nontransfected islets. Islets transfected with the control E1A mutant Ad5- d1312 vector (containing no E1A-12S or E1A-13S segments) were similar to non- transfected islets in their characteristics. We found that the E1A-12S transfected islets maintained greater viability, insulin granule structure, and glucose-induced insulin responsiveness over a 6-week period compared with mock or control islets. At 6 weeks of culture, the E1A-12S transfected islets also had fewer apoptotic cells compared with nontransfected islets. These data suggest that adenovirus E1A-12S can extend the functional life span of cultured rat pancreatic islets.
Original language | English (US) |
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Pages (from-to) | 183-192 |
Number of pages | 10 |
Journal | Pancreas |
Volume | 19 |
Issue number | 2 |
DOIs | |
State | Published - Aug 1999 |
Keywords
- E1A-12S
- Glucose
- Insulin
- Islets
- Pancreas
- Rat
- Retrovirus
- Transfection
ASJC Scopus subject areas
- Internal Medicine
- Endocrinology, Diabetes and Metabolism
- Hepatology
- Endocrinology