Proliferative index of human luteinized granulosa cells varies as a function of ovarian reserve

David Seifer, Colette Charland, David Berlinsky, Alan S. Penzias, Ray V. Haning, Frederick Naftolin, Barbara E. Barker

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

OBJECTIVE: We examined whether, the proliferative index of luteinized granulosa cells, as determined by flow cytometry, varied as a function of a woman's ovarian reserve, as reflected by follicular-phase day 3 serum follicle-stimulating hormone. STUDY DESIGN: This prospective cohort study consisted of 19 women of similar chronologic age preparing for in vitro fertilization-embryo who met specific day 3 serum follicle-stimulating hormone criteria. The "low follicle-stimulating hormone" group consisted of 11 women with day 3 serum follicle-stimulating hormone levels ≤ IU/L. The "high follicle-stimulating hormone" group consisted of eight women with day 3 serum follicle-stimulating hormone levels ≥ 18 IU/L. A total of 56 preovulatory follicles containing ≥ 104 luteinized granulosa cells were examined by flow cytometry. The low follicle-stimulating hormone group was compared with the high follicle-stimulating hormone group to examine proliferative index as a function of serum day 3 follicle-stimulating hormone levels. RESULTS: The low follicle-stimulating hormone group had a greater prolferative index (11.1 % ± 0.4%) than did the high follicle-stimulating hormone group (8.3% ± 0.6%), p <0.001). This study demonstrates that in spite of the same chronologic age, iuteinized granulosa cells from preovulatory follicles of women with day 3 serum follicle-stimulating hormone levels ≥ 18 IU/L have a 25% decreased proliferative index compared with luteinized granulosa cells from women with day 3 serum follicle-stimulating hormone levels ≤ 6. CONCLUSIONS: This suggests that granulosa cell proliferation is influenced by ovarian reserve and may explain in part the more favorable response to ovulation induction protocols that younger women demonstrate compared with women of more advanced reproductive age.

Original languageEnglish (US)
Pages (from-to)1531-1535
Number of pages5
JournalAmerican Journal of Obstetrics and Gynecology
Volume169
Issue number6
DOIs
StatePublished - 1993
Externally publishedYes

Fingerprint

Granulosa Cells
Follicle Stimulating Hormone
Serum
Ovarian Reserve
Flow Cytometry
Follicular Phase
Ovulation Induction
Fertilization in Vitro
Cohort Studies
Embryonic Structures

Keywords

  • flow cytometry
  • Granulosa cell
  • ovarian reserve
  • proliferative index

ASJC Scopus subject areas

  • Medicine(all)
  • Obstetrics and Gynecology

Cite this

Seifer, D., Charland, C., Berlinsky, D., Penzias, A. S., Haning, R. V., Naftolin, F., & Barker, B. E. (1993). Proliferative index of human luteinized granulosa cells varies as a function of ovarian reserve. American Journal of Obstetrics and Gynecology, 169(6), 1531-1535. https://doi.org/10.1016/0002-9378(93)90430-Q

Proliferative index of human luteinized granulosa cells varies as a function of ovarian reserve. / Seifer, David; Charland, Colette; Berlinsky, David; Penzias, Alan S.; Haning, Ray V.; Naftolin, Frederick; Barker, Barbara E.

In: American Journal of Obstetrics and Gynecology, Vol. 169, No. 6, 1993, p. 1531-1535.

Research output: Contribution to journalArticle

Seifer, D, Charland, C, Berlinsky, D, Penzias, AS, Haning, RV, Naftolin, F & Barker, BE 1993, 'Proliferative index of human luteinized granulosa cells varies as a function of ovarian reserve', American Journal of Obstetrics and Gynecology, vol. 169, no. 6, pp. 1531-1535. https://doi.org/10.1016/0002-9378(93)90430-Q
Seifer, David ; Charland, Colette ; Berlinsky, David ; Penzias, Alan S. ; Haning, Ray V. ; Naftolin, Frederick ; Barker, Barbara E. / Proliferative index of human luteinized granulosa cells varies as a function of ovarian reserve. In: American Journal of Obstetrics and Gynecology. 1993 ; Vol. 169, No. 6. pp. 1531-1535.
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abstract = "OBJECTIVE: We examined whether, the proliferative index of luteinized granulosa cells, as determined by flow cytometry, varied as a function of a woman's ovarian reserve, as reflected by follicular-phase day 3 serum follicle-stimulating hormone. STUDY DESIGN: This prospective cohort study consisted of 19 women of similar chronologic age preparing for in vitro fertilization-embryo who met specific day 3 serum follicle-stimulating hormone criteria. The {"}low follicle-stimulating hormone{"} group consisted of 11 women with day 3 serum follicle-stimulating hormone levels ≤ IU/L. The {"}high follicle-stimulating hormone{"} group consisted of eight women with day 3 serum follicle-stimulating hormone levels ≥ 18 IU/L. A total of 56 preovulatory follicles containing ≥ 104 luteinized granulosa cells were examined by flow cytometry. The low follicle-stimulating hormone group was compared with the high follicle-stimulating hormone group to examine proliferative index as a function of serum day 3 follicle-stimulating hormone levels. RESULTS: The low follicle-stimulating hormone group had a greater prolferative index (11.1 {\%} ± 0.4{\%}) than did the high follicle-stimulating hormone group (8.3{\%} ± 0.6{\%}), p <0.001). This study demonstrates that in spite of the same chronologic age, iuteinized granulosa cells from preovulatory follicles of women with day 3 serum follicle-stimulating hormone levels ≥ 18 IU/L have a 25{\%} decreased proliferative index compared with luteinized granulosa cells from women with day 3 serum follicle-stimulating hormone levels ≤ 6. CONCLUSIONS: This suggests that granulosa cell proliferation is influenced by ovarian reserve and may explain in part the more favorable response to ovulation induction protocols that younger women demonstrate compared with women of more advanced reproductive age.",
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AU - Naftolin, Frederick

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