Prolactin synthesis in primary cultures of pituitary cells

Regulation by estradiol

M. E. Lieberman, Richard Maurer, P. Claude, J. Gorski

Research output: Contribution to journalArticle

82 Citations (Scopus)

Abstract

Pituitary cells cultured with estradiol respond by a specific increase in prolactin synthesis. Extensive inhibition of DNA synthesis (61-78%) with hydroxyurea or cytosine arabinoside resulted in only 28-33% decrease in estrogen-induced prolactin synthesis. To assess the role of prolactin cell proliferation in the estrogen-induced response, mammotrophs were identified by immunocytochemistry. Cultures treated with estradiol for 1, 2 or 5 days contained 101 ± 1, 113 ± 2 and 132 ± 1% of the number of mammotrophs in controls. Estradiol treatment for corresponding periods resulted in prolactin synthesis representing 94 ± 5,144 ± 11 and 270 ± 22% of controls and prolactin mRNA levels representing 115 ± 7, 160 ± 7 and 322 ± 22% of controls. Thus estrogen caused a considerable increase in prolactin synthesis which paralleled the increase in prolactin mRNA levels and a much smaller relative increase in the number of mammotrophs. We conclude that regulation of prolactin synthesis by estrogen is mediated predominantly but not exclusively through stimulation of gene expression in existing pituitary cells.

Original languageEnglish (US)
Pages (from-to)277-294
Number of pages18
JournalMolecular and Cellular Endocrinology
Volume25
Issue number3
DOIs
StatePublished - 1982
Externally publishedYes

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Primary Cell Culture
Prolactin
Estradiol
Estrogens
Messenger RNA
Hydroxyurea
Cytarabine
Cell proliferation
Gene expression
Cultured Cells
Immunohistochemistry
Cell Proliferation
Gene Expression
DNA

Keywords

  • DNA synthesis
  • immunocytochemistry
  • mammotroph proliferation
  • prolactin mRNA

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Prolactin synthesis in primary cultures of pituitary cells : Regulation by estradiol. / Lieberman, M. E.; Maurer, Richard; Claude, P.; Gorski, J.

In: Molecular and Cellular Endocrinology, Vol. 25, No. 3, 1982, p. 277-294.

Research output: Contribution to journalArticle

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abstract = "Pituitary cells cultured with estradiol respond by a specific increase in prolactin synthesis. Extensive inhibition of DNA synthesis (61-78{\%}) with hydroxyurea or cytosine arabinoside resulted in only 28-33{\%} decrease in estrogen-induced prolactin synthesis. To assess the role of prolactin cell proliferation in the estrogen-induced response, mammotrophs were identified by immunocytochemistry. Cultures treated with estradiol for 1, 2 or 5 days contained 101 ± 1, 113 ± 2 and 132 ± 1{\%} of the number of mammotrophs in controls. Estradiol treatment for corresponding periods resulted in prolactin synthesis representing 94 ± 5,144 ± 11 and 270 ± 22{\%} of controls and prolactin mRNA levels representing 115 ± 7, 160 ± 7 and 322 ± 22{\%} of controls. Thus estrogen caused a considerable increase in prolactin synthesis which paralleled the increase in prolactin mRNA levels and a much smaller relative increase in the number of mammotrophs. We conclude that regulation of prolactin synthesis by estrogen is mediated predominantly but not exclusively through stimulation of gene expression in existing pituitary cells.",
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AB - Pituitary cells cultured with estradiol respond by a specific increase in prolactin synthesis. Extensive inhibition of DNA synthesis (61-78%) with hydroxyurea or cytosine arabinoside resulted in only 28-33% decrease in estrogen-induced prolactin synthesis. To assess the role of prolactin cell proliferation in the estrogen-induced response, mammotrophs were identified by immunocytochemistry. Cultures treated with estradiol for 1, 2 or 5 days contained 101 ± 1, 113 ± 2 and 132 ± 1% of the number of mammotrophs in controls. Estradiol treatment for corresponding periods resulted in prolactin synthesis representing 94 ± 5,144 ± 11 and 270 ± 22% of controls and prolactin mRNA levels representing 115 ± 7, 160 ± 7 and 322 ± 22% of controls. Thus estrogen caused a considerable increase in prolactin synthesis which paralleled the increase in prolactin mRNA levels and a much smaller relative increase in the number of mammotrophs. We conclude that regulation of prolactin synthesis by estrogen is mediated predominantly but not exclusively through stimulation of gene expression in existing pituitary cells.

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