Prolactin expression in the sheep brain

Charles Roselli, Sven Bocklandt, Henry L. Stadelman, Teri Wadsworth, Eric Vilain, Fred Stormshak

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Accumulating evidence in rodents suggests that a prolactin locally synthesized and released within the brain can act together with that taken up from the circulation to modulate neuroendocrine responses. The present study was designed to identify the regional patterns of prolactin expression in the adult and developing sheep brain. Specifically, we tested the hypothesis that prolactin is expressed in regions of the adult and fetal sheep brain that are critical in the development of neuroendocrine homeostatic and behavioral functions. The expression of prolactin protein in sheep brain was demonstrated by Western blot analysis and brain prolactin mRNA was detected and sequenced using RT-PCR. In situ hybridization histochemistry revealed that prolactin mRNA was expressed in the medial preoptic area, periventricular preoptic nucleus, bed nucleus of the stria terminalis, and in the paraventricular nucleus of the hypothalamus, particularly the ventral region. The neuroanatomical distribution of prolactin mRNA was best visualized in the fetus and prolactin-immunoreactive neurons could also be identified in late gestation fetuses. Brain prolactin mRNA was expressed as early as day 60 of gestation and increased as the fetus aged and peaked at day 135 (term = 147 days). Prolactin mRNA expression did not exhibit a sex difference in the preoptic area, but in the amygdala prolactin mRNA was significantly higher in females than in males at day 100 of gestation. We conclude that prolactin expressed in adult and fetal sheep brain could be involved in neurodevelopment and/or modulation of the neuroendocrine stress axis, although it is too early to rule out other possibilities given the diverse actions that have been attributed to prolactin.

Original languageEnglish (US)
Pages (from-to)206-215
Number of pages10
JournalNeuroendocrinology
Volume87
Issue number4
DOIs
StatePublished - May 2008

Fingerprint

Prolactin
Sheep
Brain
Messenger RNA
Preoptic Area
Fetus
Pregnancy
Septal Nuclei
Paraventricular Hypothalamic Nucleus
Amygdala
Sex Characteristics
Hypothalamus
In Situ Hybridization
Rodentia
Western Blotting
Neurons
Polymerase Chain Reaction

Keywords

  • Amygdala
  • Hypothalamus
  • Preoptic area
  • Prolactin

ASJC Scopus subject areas

  • Endocrinology
  • Neuroscience(all)

Cite this

Roselli, C., Bocklandt, S., Stadelman, H. L., Wadsworth, T., Vilain, E., & Stormshak, F. (2008). Prolactin expression in the sheep brain. Neuroendocrinology, 87(4), 206-215. https://doi.org/10.1159/000114643

Prolactin expression in the sheep brain. / Roselli, Charles; Bocklandt, Sven; Stadelman, Henry L.; Wadsworth, Teri; Vilain, Eric; Stormshak, Fred.

In: Neuroendocrinology, Vol. 87, No. 4, 05.2008, p. 206-215.

Research output: Contribution to journalArticle

Roselli, C, Bocklandt, S, Stadelman, HL, Wadsworth, T, Vilain, E & Stormshak, F 2008, 'Prolactin expression in the sheep brain', Neuroendocrinology, vol. 87, no. 4, pp. 206-215. https://doi.org/10.1159/000114643
Roselli C, Bocklandt S, Stadelman HL, Wadsworth T, Vilain E, Stormshak F. Prolactin expression in the sheep brain. Neuroendocrinology. 2008 May;87(4):206-215. https://doi.org/10.1159/000114643
Roselli, Charles ; Bocklandt, Sven ; Stadelman, Henry L. ; Wadsworth, Teri ; Vilain, Eric ; Stormshak, Fred. / Prolactin expression in the sheep brain. In: Neuroendocrinology. 2008 ; Vol. 87, No. 4. pp. 206-215.
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