Proinflammatory phenotype of perivascular adipocytes: Influence of high-fat feeding

Tapan K. Chatterjee, Lynn L. Stoll, Gerene M. Denning, Allan Harrelson, Andra L. Blomkalns, Gila Idelman, Florence G. Rothenberg, Bonnie Neltner, Sara A. Romig-Martin, Eric W. Dickson, Steven Rudich, Neal L. Weintraub

    Research output: Contribution to journalArticle

    335 Scopus citations

    Abstract

    Adipose tissue depots originate from distinct precursor cells, are functionally diverse, and modulate disease processes in a depot-specific manner. However, the functional properties of perivascular adipocytes, and their influence on disease of the blood vessel wall, remain to be determined. We show that human coronary perivascular adipocytes exhibit a reduced state of adipocytic differentiation as compared with adipocytes derived from subcutaneous and visceral (perirenal) adipose depots. Secretion of antiinflammatory adiponectin is markedly reduced, whereas that of proinflammatory cytokines interleukin-6, interleukin-8, and monocyte chemoattractant protein-1, is markedly increased in perivascular adipocytes. These depot-specific differences in adipocyte function are demonstrable in both freshly isolated adipose tissues and in vitro-differentiated adipocytes. Murine aortic arch perivascular adipose tissues likewise express lower levels of adipocyte-associated genes as compared with subcutaneous and visceral adipose tissues. Moreover, 2 weeks of high-fat feeding caused further reductions in adipocyte-associated gene expression, while upregulating proinflammatory gene expression, in perivascular adipose tissues. These changes were observed in the absence of macrophage recruitment to the perivascular adipose depot. We conclude that perivascular adipocytes exhibit reduced differentiation and a heightened proinflammatory state, properties that are intrinsic to the adipocytes residing in this depot. Dysfunction of perivascular adipose tissue induced by fat feeding suggests that this unique adipose depot is capable of linking metabolic signals to inflammation in the blood vessel wall.

    Original languageEnglish (US)
    Pages (from-to)541-549
    Number of pages9
    JournalCirculation research
    Volume104
    Issue number4
    DOIs
    StatePublished - Feb 27 2009

    Keywords

    • Adipocytes
    • Adipokines
    • Adventitia
    • Cytokines
    • Perivascular adipose tissue

    ASJC Scopus subject areas

    • Physiology
    • Cardiology and Cardiovascular Medicine

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  • Cite this

    Chatterjee, T. K., Stoll, L. L., Denning, G. M., Harrelson, A., Blomkalns, A. L., Idelman, G., Rothenberg, F. G., Neltner, B., Romig-Martin, S. A., Dickson, E. W., Rudich, S., & Weintraub, N. L. (2009). Proinflammatory phenotype of perivascular adipocytes: Influence of high-fat feeding. Circulation research, 104(4), 541-549. https://doi.org/10.1161/CIRCRESAHA.108.182998