TY - JOUR
T1 - Progesterone production by luteal cells isolated from cynomolgus monkeys
T2 - Effects of gonadotropin and prolactin during acute incubation and cell culture
AU - Stouffer, Richard L.
AU - Coensgen, Judy L.
AU - Hodgen, Gary D.
N1 - Funding Information:
The expert assistance of Mrs. Drue Morgan-Birch of the University of Arizona Health Sciences Center, and Mr. Donald Barber and the animal care staff of the Pregnancy Research Branch, NIH is greatly appreciated. Purified hPRL was a gift from the National Pituitary Agency, sponsored by the NIAMDD. This work was supported in part by NIH Grant HL-07249.
PY - 1980/5
Y1 - 1980/5
N2 - Corpus luteum function in cynomolgus monkeys (Macaca fascicularis) during the menstrual cycle and immediately following parturition was evaluated through in vitro studies on progesterone production by dispersed luteal cells in the presence and absence of human chorionic gonadotropin (hCG) or human prolactin (hPRL). Luteal cells isolated between days 17-20 of the menstrual cycle secreted progesterone (P) during short-term incubation (21.6 ± 1.2 ngP/ml/5 × 104 cells/3 hr, X ± S.E., n = 7) and responded to the addition of 1-100 ng hCG with a significant (p < 0.05) increase in P secretion. Cells removed the day of delivery secreted large, but variable (27.9-222 ng/ml, n = 4) amounts of P during short-term incubation. Moreover, hCG (100 ng/ml) stimulation of P production by cells at delivery (176 ± 19% of control) was less than that of cells from the cycle (336 ± 65%). The presence of hPRL (2.5-5000 ng/ml) failed to influence P secretion by luteal cells during short-term incubation in the presence or absence of hCG. P production by luteal cells obtained following delivery declined markedly during 8 days of culture in Ham's F10 medium: 10% fetal calf serum. Continual exposure to 100 ng/ml of hCG or hPRL failed to influence P secretion through Day 2 of culture. Thereafter hCG progressively enhanced (p < 0.05) P secretion to 613% of control levels at Day 8 of culture. In contrast, hPRL significantly increased P secretion (163% of control levels, p < 0.05) between Day 2-4 of culture, but the stimulatory effect diminished thereafter. The data indicate that dispersed luteal cells from the cynomolgus monkey provide a suitable model for in vitro studies on the primate corpus luteum during the menstrual cycle, pregnancy, and the puerperium, including further investigation of the possible roles of gonadotropin and PRL in the regulation of luteal function in primates.
AB - Corpus luteum function in cynomolgus monkeys (Macaca fascicularis) during the menstrual cycle and immediately following parturition was evaluated through in vitro studies on progesterone production by dispersed luteal cells in the presence and absence of human chorionic gonadotropin (hCG) or human prolactin (hPRL). Luteal cells isolated between days 17-20 of the menstrual cycle secreted progesterone (P) during short-term incubation (21.6 ± 1.2 ngP/ml/5 × 104 cells/3 hr, X ± S.E., n = 7) and responded to the addition of 1-100 ng hCG with a significant (p < 0.05) increase in P secretion. Cells removed the day of delivery secreted large, but variable (27.9-222 ng/ml, n = 4) amounts of P during short-term incubation. Moreover, hCG (100 ng/ml) stimulation of P production by cells at delivery (176 ± 19% of control) was less than that of cells from the cycle (336 ± 65%). The presence of hPRL (2.5-5000 ng/ml) failed to influence P secretion by luteal cells during short-term incubation in the presence or absence of hCG. P production by luteal cells obtained following delivery declined markedly during 8 days of culture in Ham's F10 medium: 10% fetal calf serum. Continual exposure to 100 ng/ml of hCG or hPRL failed to influence P secretion through Day 2 of culture. Thereafter hCG progressively enhanced (p < 0.05) P secretion to 613% of control levels at Day 8 of culture. In contrast, hPRL significantly increased P secretion (163% of control levels, p < 0.05) between Day 2-4 of culture, but the stimulatory effect diminished thereafter. The data indicate that dispersed luteal cells from the cynomolgus monkey provide a suitable model for in vitro studies on the primate corpus luteum during the menstrual cycle, pregnancy, and the puerperium, including further investigation of the possible roles of gonadotropin and PRL in the regulation of luteal function in primates.
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U2 - 10.1016/S0039-128X(80)80006-7
DO - 10.1016/S0039-128X(80)80006-7
M3 - Article
C2 - 6771895
AN - SCOPUS:0019224076
SN - 0039-128X
VL - 35
SP - 523
EP - 532
JO - Steroids
JF - Steroids
IS - 5
ER -