Progesterone production by luteal cells isolated from cynomolgus monkeys: Effects of gonadotropin and prolactin during acute incubation and cell culture

Richard Stouffer, Judy L. Coensgen, Gary D. Hodgen

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Corpus luteum function in cynomolgus monkeys (Macaca fascicularis) during the menstrual cycle and immediately following parturition was evaluated through in vitro studies on progesterone production by dispersed luteal cells in the presence and absence of human chorionic gonadotropin (hCG) or human prolactin (hPRL). Luteal cells isolated between days 17-20 of the menstrual cycle secreted progesterone (P) during short-term incubation (21.6 ± 1.2 ngP/ml/5 × 104 cells/3 hr, X ± S.E., n = 7) and responded to the addition of 1-100 ng hCG with a significant (p <0.05) increase in P secretion. Cells removed the day of delivery secreted large, but variable (27.9-222 ng/ml, n = 4) amounts of P during short-term incubation. Moreover, hCG (100 ng/ml) stimulation of P production by cells at delivery (176 ± 19% of control) was less than that of cells from the cycle (336 ± 65%). The presence of hPRL (2.5-5000 ng/ml) failed to influence P secretion by luteal cells during short-term incubation in the presence or absence of hCG. P production by luteal cells obtained following delivery declined markedly during 8 days of culture in Ham's F10 medium: 10% fetal calf serum. Continual exposure to 100 ng/ml of hCG or hPRL failed to influence P secretion through Day 2 of culture. Thereafter hCG progressively enhanced (p <0.05) P secretion to 613% of control levels at Day 8 of culture. In contrast, hPRL significantly increased P secretion (163% of control levels, p <0.05) between Day 2-4 of culture, but the stimulatory effect diminished thereafter. The data indicate that dispersed luteal cells from the cynomolgus monkey provide a suitable model for in vitro studies on the primate corpus luteum during the menstrual cycle, pregnancy, and the puerperium, including further investigation of the possible roles of gonadotropin and PRL in the regulation of luteal function in primates.

Original languageEnglish (US)
Pages (from-to)523-532
Number of pages10
JournalSteroids
Volume35
Issue number5
DOIs
StatePublished - 1980
Externally publishedYes

Fingerprint

Luteal Cells
Macaca fascicularis
Chorionic Gonadotropin
Gonadotropins
Cell culture
Prolactin
Progesterone
Cell Culture Techniques
Corpus Luteum
Menstrual Cycle
Level control
Primates
Postpartum Period
Cell Cycle
Parturition
Pregnancy
Serum

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology
  • Molecular Biology

Cite this

Progesterone production by luteal cells isolated from cynomolgus monkeys : Effects of gonadotropin and prolactin during acute incubation and cell culture. / Stouffer, Richard; Coensgen, Judy L.; Hodgen, Gary D.

In: Steroids, Vol. 35, No. 5, 1980, p. 523-532.

Research output: Contribution to journalArticle

@article{488e0c4e3f954738a5a2c2aac18b1a35,
title = "Progesterone production by luteal cells isolated from cynomolgus monkeys: Effects of gonadotropin and prolactin during acute incubation and cell culture",
abstract = "Corpus luteum function in cynomolgus monkeys (Macaca fascicularis) during the menstrual cycle and immediately following parturition was evaluated through in vitro studies on progesterone production by dispersed luteal cells in the presence and absence of human chorionic gonadotropin (hCG) or human prolactin (hPRL). Luteal cells isolated between days 17-20 of the menstrual cycle secreted progesterone (P) during short-term incubation (21.6 ± 1.2 ngP/ml/5 × 104 cells/3 hr, X ± S.E., n = 7) and responded to the addition of 1-100 ng hCG with a significant (p <0.05) increase in P secretion. Cells removed the day of delivery secreted large, but variable (27.9-222 ng/ml, n = 4) amounts of P during short-term incubation. Moreover, hCG (100 ng/ml) stimulation of P production by cells at delivery (176 ± 19{\%} of control) was less than that of cells from the cycle (336 ± 65{\%}). The presence of hPRL (2.5-5000 ng/ml) failed to influence P secretion by luteal cells during short-term incubation in the presence or absence of hCG. P production by luteal cells obtained following delivery declined markedly during 8 days of culture in Ham's F10 medium: 10{\%} fetal calf serum. Continual exposure to 100 ng/ml of hCG or hPRL failed to influence P secretion through Day 2 of culture. Thereafter hCG progressively enhanced (p <0.05) P secretion to 613{\%} of control levels at Day 8 of culture. In contrast, hPRL significantly increased P secretion (163{\%} of control levels, p <0.05) between Day 2-4 of culture, but the stimulatory effect diminished thereafter. The data indicate that dispersed luteal cells from the cynomolgus monkey provide a suitable model for in vitro studies on the primate corpus luteum during the menstrual cycle, pregnancy, and the puerperium, including further investigation of the possible roles of gonadotropin and PRL in the regulation of luteal function in primates.",
author = "Richard Stouffer and Coensgen, {Judy L.} and Hodgen, {Gary D.}",
year = "1980",
doi = "10.1016/S0039-128X(80)80006-7",
language = "English (US)",
volume = "35",
pages = "523--532",
journal = "Steroids",
issn = "0039-128X",
publisher = "Elsevier Inc.",
number = "5",

}

TY - JOUR

T1 - Progesterone production by luteal cells isolated from cynomolgus monkeys

T2 - Effects of gonadotropin and prolactin during acute incubation and cell culture

AU - Stouffer, Richard

AU - Coensgen, Judy L.

AU - Hodgen, Gary D.

PY - 1980

Y1 - 1980

N2 - Corpus luteum function in cynomolgus monkeys (Macaca fascicularis) during the menstrual cycle and immediately following parturition was evaluated through in vitro studies on progesterone production by dispersed luteal cells in the presence and absence of human chorionic gonadotropin (hCG) or human prolactin (hPRL). Luteal cells isolated between days 17-20 of the menstrual cycle secreted progesterone (P) during short-term incubation (21.6 ± 1.2 ngP/ml/5 × 104 cells/3 hr, X ± S.E., n = 7) and responded to the addition of 1-100 ng hCG with a significant (p <0.05) increase in P secretion. Cells removed the day of delivery secreted large, but variable (27.9-222 ng/ml, n = 4) amounts of P during short-term incubation. Moreover, hCG (100 ng/ml) stimulation of P production by cells at delivery (176 ± 19% of control) was less than that of cells from the cycle (336 ± 65%). The presence of hPRL (2.5-5000 ng/ml) failed to influence P secretion by luteal cells during short-term incubation in the presence or absence of hCG. P production by luteal cells obtained following delivery declined markedly during 8 days of culture in Ham's F10 medium: 10% fetal calf serum. Continual exposure to 100 ng/ml of hCG or hPRL failed to influence P secretion through Day 2 of culture. Thereafter hCG progressively enhanced (p <0.05) P secretion to 613% of control levels at Day 8 of culture. In contrast, hPRL significantly increased P secretion (163% of control levels, p <0.05) between Day 2-4 of culture, but the stimulatory effect diminished thereafter. The data indicate that dispersed luteal cells from the cynomolgus monkey provide a suitable model for in vitro studies on the primate corpus luteum during the menstrual cycle, pregnancy, and the puerperium, including further investigation of the possible roles of gonadotropin and PRL in the regulation of luteal function in primates.

AB - Corpus luteum function in cynomolgus monkeys (Macaca fascicularis) during the menstrual cycle and immediately following parturition was evaluated through in vitro studies on progesterone production by dispersed luteal cells in the presence and absence of human chorionic gonadotropin (hCG) or human prolactin (hPRL). Luteal cells isolated between days 17-20 of the menstrual cycle secreted progesterone (P) during short-term incubation (21.6 ± 1.2 ngP/ml/5 × 104 cells/3 hr, X ± S.E., n = 7) and responded to the addition of 1-100 ng hCG with a significant (p <0.05) increase in P secretion. Cells removed the day of delivery secreted large, but variable (27.9-222 ng/ml, n = 4) amounts of P during short-term incubation. Moreover, hCG (100 ng/ml) stimulation of P production by cells at delivery (176 ± 19% of control) was less than that of cells from the cycle (336 ± 65%). The presence of hPRL (2.5-5000 ng/ml) failed to influence P secretion by luteal cells during short-term incubation in the presence or absence of hCG. P production by luteal cells obtained following delivery declined markedly during 8 days of culture in Ham's F10 medium: 10% fetal calf serum. Continual exposure to 100 ng/ml of hCG or hPRL failed to influence P secretion through Day 2 of culture. Thereafter hCG progressively enhanced (p <0.05) P secretion to 613% of control levels at Day 8 of culture. In contrast, hPRL significantly increased P secretion (163% of control levels, p <0.05) between Day 2-4 of culture, but the stimulatory effect diminished thereafter. The data indicate that dispersed luteal cells from the cynomolgus monkey provide a suitable model for in vitro studies on the primate corpus luteum during the menstrual cycle, pregnancy, and the puerperium, including further investigation of the possible roles of gonadotropin and PRL in the regulation of luteal function in primates.

UR - http://www.scopus.com/inward/record.url?scp=0019224076&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0019224076&partnerID=8YFLogxK

U2 - 10.1016/S0039-128X(80)80006-7

DO - 10.1016/S0039-128X(80)80006-7

M3 - Article

C2 - 6771895

AN - SCOPUS:0019224076

VL - 35

SP - 523

EP - 532

JO - Steroids

JF - Steroids

SN - 0039-128X

IS - 5

ER -