Progesterone production by luteal cells isolated from cynomolgus monkeys: Effects of gonadotropin and prolactin during acute incubation and cell culture

Richard L. Stouffer, Judy L. Coensgen, Gary D. Hodgen

    Research output: Contribution to journalArticle

    8 Scopus citations

    Abstract

    Corpus luteum function in cynomolgus monkeys (Macaca fascicularis) during the menstrual cycle and immediately following parturition was evaluated through in vitro studies on progesterone production by dispersed luteal cells in the presence and absence of human chorionic gonadotropin (hCG) or human prolactin (hPRL). Luteal cells isolated between days 17-20 of the menstrual cycle secreted progesterone (P) during short-term incubation (21.6 ± 1.2 ngP/ml/5 × 104 cells/3 hr, X ± S.E., n = 7) and responded to the addition of 1-100 ng hCG with a significant (p < 0.05) increase in P secretion. Cells removed the day of delivery secreted large, but variable (27.9-222 ng/ml, n = 4) amounts of P during short-term incubation. Moreover, hCG (100 ng/ml) stimulation of P production by cells at delivery (176 ± 19% of control) was less than that of cells from the cycle (336 ± 65%). The presence of hPRL (2.5-5000 ng/ml) failed to influence P secretion by luteal cells during short-term incubation in the presence or absence of hCG. P production by luteal cells obtained following delivery declined markedly during 8 days of culture in Ham's F10 medium: 10% fetal calf serum. Continual exposure to 100 ng/ml of hCG or hPRL failed to influence P secretion through Day 2 of culture. Thereafter hCG progressively enhanced (p < 0.05) P secretion to 613% of control levels at Day 8 of culture. In contrast, hPRL significantly increased P secretion (163% of control levels, p < 0.05) between Day 2-4 of culture, but the stimulatory effect diminished thereafter. The data indicate that dispersed luteal cells from the cynomolgus monkey provide a suitable model for in vitro studies on the primate corpus luteum during the menstrual cycle, pregnancy, and the puerperium, including further investigation of the possible roles of gonadotropin and PRL in the regulation of luteal function in primates.

    Original languageEnglish (US)
    Pages (from-to)523-532
    Number of pages10
    JournalSteroids
    Volume35
    Issue number5
    DOIs
    StatePublished - May 1980

    ASJC Scopus subject areas

    • Biochemistry
    • Molecular Biology
    • Endocrinology
    • Pharmacology
    • Clinical Biochemistry
    • Organic Chemistry

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